1. The metabolism of erythropoietin in the normal and uraemic rabbit.
- Author
-
Brown JH, Lappin TR, Elder GE, Bridges JM, and McGeown MG
- Subjects
- Animals, Disease Models, Animal, Female, Half-Life, In Vitro Techniques, Male, Nephrectomy, Rabbits, Uremia etiology, Erythropoietin blood, Uremia blood
- Abstract
The metabolism of recombinant human erythropoietin (rHuEpo) labelled with 125I has been investigated in five normal and nine 5/6-nephrectomised rabbits. The plasma erythropoietin half-life was significantly prolonged at 5.1 +/- 1.2 h (mean +/- SD) in the 5/6-nephrectomised rabbits, compared to 3.0 +/- 0.4 h in sham-operated controls (P less than 0.001). The disappearance of 125I-labelled rHuEpo is biphasic. Examination of serum by fast protein liquid chromatography (FPLC) following administration of 125I-labelled rHuEpo by FPLC showed a single peak of radioactivity in all rabbits except two of the nephrectomised group. In serum from both of these animals a second labelled peak was found, corresponding to material of MW 200,000-250,000 D. We conclude that the 5/6-nephrectomised rabbit provides a stable model for the study of hormonal metabolism in chronic renal failure.
- Published
- 1990
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