1. The Prevalence of Legionella Species as a Co-pathogen in HIV-Associated Community-Acquired Pneumonia
- Author
-
Lázaro Vélez, Zulma Vanessa Rueda, Breanne M. Head, Tamara Burdz, Yoav Keynan, Adriana Trajtman, and Kathryn Bernard
- Subjects
Pathology ,medicine.medical_specialty ,biology ,business.industry ,Legionella ,Human immunodeficiency virus (HIV) ,Poster Abstract ,biology.organism_classification ,medicine.disease_cause ,Pathogenicity ,medicine.disease ,bacterial infections and mycoses ,Microbiology ,respiratory tract diseases ,Therapeutic immunosuppression ,Abstracts ,Infectious Diseases ,Oncology ,Community-acquired pneumonia ,medicine ,Coinfection ,Legionella species ,business ,Pathogen - Abstract
Background Due to the lack of clinical suspicion, poor diagnostic performance, increased patient immunosuppression, and the increased chance of co-infection, HIV-associated Legionella community-acquired pneumonia (CAP) is currently under-reported. Thus, this study aimed to determine the frequency of Legionella in CAP-infected HIV patients. Methods Following initial diagnosis, DNA extracted from bronchoalveolar lavage (BAL) from CAP-infected HIV patients hospitalized at Hospital San Vicente Fundación in Medellin, Colombia were assayed for the presence of Legionella species (PAN Legionella, L. Anisa, L. bozemanii, L. micdadei, L. pneumophila and L. pneumophila serogroup 1) using singleplex real-time PCR (qPCR). Results were validated with agarose gel electrophoresis and reconfirmed using pre-amplification qPCR. Results Of the 59 HIV-infected individuals in the study, majority were non-smokers (64.4%), male (77.9%), and highly immunosuppressed (CD4 cell count
- Published
- 2017