15 results on '"Javier Muñoz"'
Search Results
2. P2.21: Protection of Normal Metabolic Function of Healthy Pig Livers Following Prolonged Normothermic Ex-situ Perfusion
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Jordi Vengohechea Llorens, Javier Muñoz, Xiran He, Mingju Liang, Josep Maria Sanahuja, Marina Vendrell, Felipe León, Joaquim Albiol, Li Lin, Xiaoyu Tan, Feng Huo, Amelia Judith Hessheimer, and Constantino Fondevila
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Transplantation - Published
- 2022
3. Torque Teno Virus Is Associated With the State of Immune Suppression Early After Liver Transplantation
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Marta Martínez-Picola, Pablo Ruiz, Giorgos Koutsoudakis, Lydia Sastre, Miquel Navasa, Miguel Henrique de Almeida Santana, Javier Muñoz, Sofía Pérez-del-Pulgar, Gonzalo Crespo, and Jordi Colmenero
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Graft Rejection ,Male ,Torque teno virus ,Biopsy ,medicine.medical_treatment ,Cytomegalovirus ,030230 surgery ,Liver transplantation ,law.invention ,0302 clinical medicine ,law ,Postoperative Period ,Prospective Studies ,Polymerase chain reaction ,Immunosuppression ,DNA virus ,Middle Aged ,Viral Load ,Allografts ,Real-time polymerase chain reaction ,Liver ,Cytomegalovirus Infections ,Female ,030211 gastroenterology & hepatology ,Viral load ,Adult ,Viremia ,Opportunistic Infections ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Immune Tolerance ,medicine ,Humans ,Aged ,Retrospective Studies ,Immunosuppression Therapy ,Transplantation ,Host Microbial Interactions ,Hepatology ,business.industry ,medicine.disease ,Liver Transplantation ,DNA, Viral ,Immunology ,Surgery ,business ,Biomarkers ,Follow-Up Studies - Abstract
The development of noninvasive biomarkers that reflect the state of immunosuppression (IS) remains an unmet need in liver transplantation (LT). Torque Teno virus (TTV) is a highly prevalent, nonpathogenic DNA virus whose plasma levels may be associated with the immune status of the host. The aim of this study was to assess the role of TTV as a biomarker of IS in LT recipients. TTV DNA in plasma was quantified by real-time polymerase chain reaction at different time points during the first year after transplant in a prospectively followed cohort of 63 de novo LT recipients, and any correlation between TTV DNA and biopsy-proven acute cellular rejection (ACR) and opportunistic infections was then evaluated. In addition, TTV DNA was studied in 10 longterm LT recipients in monotherapy with tacrolimus, 10 tolerant recipients, and 10 healthy controls. TTV was detected in the plasma of all patients. Among the 63 LT recipients, 20 episodes of ACR were diagnosed, and there were 28 opportunistic infections, 26 of them being cytomegalovirus (CMV) infections. TTV viremia was significantly lower during ACR (4.41 versus 5.95 log10 copies/mL; P = 0.002) and significantly higher during CMV infections (5.79 versus 6.59 log10 copies/mL; P = 0.009). The area under the receiver operating characteristic curve of TTV viral load for the diagnosis of moderate ACR was 0.869, with a sensitivity and negative predictive value of 100%, respectively, for a cutoff point of 4.75 log10 copies/mL. There were no statistically significant differences in TTV DNA in either longterm or tolerant patients and healthy controls. In conclusion, plasma TTV DNA levels are associated with immune-related events after LT and could constitute a potential biomarker of the state of IS during the first months after transplant.
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- 2019
4. Heparin but not tissue plasminogen activator improves outcomes in donation after circulatory death liver transplantation in a porcine model
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Marina Vendrell, Constantino Fondevila, Luís Flores Sigüenza, Angel Recio Ruiz, Juan Carlos García-Valdecasas, Pilar Taura, Miquel Navasa, Alba Díaz, Jorge Rodríguez Lanzilotta, Amelia J. Hessheimer, Javier Muñoz, José Fuster, and Eduardo Delgado Oliver
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Male ,medicine.medical_specialty ,Time Factors ,medicine.medical_treatment ,Sus scrofa ,Anti-Inflammatory Agents ,Urology ,Bile Duct Diseases ,030230 surgery ,Liver transplantation ,Tissue plasminogen activator ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Fibrinolytic Agents ,medicine ,Animals ,Hepatectomy ,Blood Coagulation ,Transplantation ,Hepatology ,Heparin ,business.industry ,Anticoagulants ,Thrombosis ,Cytoprotection ,Circulatory death ,Liver Transplantation ,Perfusion ,Reperfusion Injury ,Tissue Plasminogen Activator ,Models, Animal ,030211 gastroenterology & hepatology ,Surgery ,Complication ,business ,medicine.drug - Abstract
Ischemic-type biliary lesions (ITBLs) arise most frequently after donation after circulatory death (DCD) liver transplantation and result in high morbidity and graft loss. Many DCD grafts are discarded out of fear for this complication. In theory, microvascular thrombi deposited during donor warm ischemia might be implicated in ITBL pathogenesis. Herein, we aim to evaluate the effects of the administration of either heparin or the fibrinolytic drug tissue plasminogen activator (TPA) as means to improve DCD liver graft quality and potentially avoid ITBL. Donor pigs were subjected to 1 hour of cardiac arrest (CA) and divided among 3 groups: no pre-arrest heparinization nor TPA during postmortem regional perfusion; no pre-arrest heparinization but TPA given during regional perfusion; and pre-arrest heparinization but no TPA during regional perfusion. In liver tissue sampled 1 hour after CA, fibrin deposition was not detected, even when heparin was not given prior to arrest. Although it was not useful to prevent microvascular clot formation, pre-arrest heparin did offer cytoprotective effects during CA and beyond, reflected in improved flows during regional perfusion and better biochemical, functional, and histological parameters during posttransplantation follow-up. In conclusion, this study demonstrates the lack of impact of TPA use in porcine DCD liver transplantation and adds to the controversy over whether the use of TPA in human DCD liver transplantation really offers any protective effect. On the other hand, when it is administered prior to CA, heparin does offer anti-inflammatory and other cytoprotective effects that help improve DCD liver graft quality. Liver Transplantation 24 665-676 2018 AASLD.
- Published
- 2018
5. OBSERVATIONS ON 24-HOUR EX SITU NORMOTHERMIC LIVER PERFUSION
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Li Lin, Joaquim Albiol, Isabel Mora, Javier Muñoz, Constantino Fondevila, Marina Vendrell, Amelia J. Hessheimer, Tan Xiaoyu, Liang Mingju, Felipe León, Huo Feng, He Xiran, and Josep Martí Sanahuja
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Transplantation ,Liver perfusion ,business.industry ,Medicine ,business ,Nuclear medicine - Published
- 2020
6. [Untitled]
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Javier Muñoz Bono, Emilio Curiel Balsera, Ricardo Fernandez, and Gabino Jimenez Perez
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medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Cardiology ,EuroSCORE ,Critical Care and Intensive Care Medicine ,business ,Cardiac surgery - Published
- 2012
7. Returning to Dialysis after Kidney Transplant Failure. Does the Dialysis Treatment Modality Influence the Survival Prognosis?
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Ana Delgado Ureña, Julie Wu, Juan Manuel Cazorla López, Javier Muñoz, Auxiliadora Mazuecos Blanca, Antonio Moreno Salazar, Carmen Minguez Mañanes, Florentino Villanego Fernández, Teresa Garcia Alvarez, and Cristhian Orellana Chávez
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Transplantation ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,030232 urology & nephrology ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,Kidney transplant failure ,Treatment modality ,Survival prognosis ,medicine ,business ,Dialysis - Published
- 2018
8. Liver Regeneration Rate Analysis in Living Donor Liver Transplantation
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Santiago Sánchez, Miquel Navasa, Constantino Fondevila, Amelia J. Hessheimer, Eduardo Delgado, David Calatayud, Juan Carlos García-Valdecasas, Víctor Molina, Lilia Martínez de la Maza, Javier Muñoz, and Josep Fuster
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Rate analysis ,Transplantation ,medicine.medical_specialty ,Chemistry ,Internal medicine ,medicine ,Living donor liver transplantation ,Gastroenterology ,Liver regeneration - Published
- 2018
9. Portal hyperperfusion: Mechanism of injury and stimulus for regeneration in porcine small-for-size transplantation
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David Calatayud, Antoni Rimola, Pilar Taura, Juan Carlos García-Valdecasas, Javier Muñoz, Amelia J. Hessheimer, José Fuster, Constantino Fondevila, Nicolás de Riva, and Olga Sánchez
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musculoskeletal diseases ,Transplantation ,medicine.medical_specialty ,Pathology ,Hepatology ,Endothelium ,business.industry ,medicine.medical_treatment ,Urology ,Hemodynamics ,Liver transplantation ,Pathogenesis ,Endothelial stem cell ,fluids and secretions ,medicine.anatomical_structure ,medicine ,Surgery ,Hepatectomy ,business ,Survival rate - Abstract
Understanding the pathogenesis of small-for-size (SFS) syndrome is critical to expanding the applicability of partial liver transplantation. We aimed to characterize its acute presentation and association with alterations in hepatic hemodynamics, microstructure, and regeneration in a porcine model. Eighteen SFS liver transplants were performed. Donors underwent 70% hepatectomy. Partial grafts were implanted into larger recipients. Whole liver transplants were also performed (n = 6). Recipients were followed until death or for 5 days. Hemodynamics were measured, and tissue was sampled intraoperatively and at the study end. Serum was sampled regularly during follow-up. Seventeen SFS transplants and 6 whole liver transplants were included. SFS grafts represented 23.2% (19.3%-25.3%) of the recipients' standard liver volume. The survival rate was 29% and 100% in the SFS and whole liver groups, respectively. The portal venous flow, pressure gradient, and resistance were significantly higher in recipients of SFS grafts versus whole livers after portal and arterial reperfusion. Arterial flow as a percentage of the total liver blood flow was significantly lower after reperfusion in SFS grafts and remained so when measured again after 5 days. Markers of endothelial cell injury increased soon after reperfusion, and those of hepatocellular injury increased later; both predicted the appearance of either graft failure or histological recovery. Proliferative activity peaked earlier and higher among nonsurvivors in the SFS group. Surviving grafts demonstrated a slower but maintained rise in regenerative activity, although metabolic activity failed to improve. In SFS transplantation in the acute setting, portal hyperperfusion is a stimulus for regeneration but may simultaneously cause irreparable endothelial injury. This porcine model not only helps to elucidate the inciting factors in SFS pathogenesis but also offers a clinically relevant means to study its prevention.
- Published
- 2010
10. Effect of propranolol on the factors promoting bacterial translocation in cirrhotic rats with ascites
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Martín Santos, Javier Muñoz, Agustin Albillos, Isabel Freile, María Pérez-Páramo, Francisca Portero, and José Ortiz-Berrocal
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medicine.medical_specialty ,Cirrhosis ,Hepatology ,Bacterial Peritonitis ,Portal venous pressure ,Chromosomal translocation ,Biology ,medicine.disease ,Intestinal absorption ,Excretion ,medicine.anatomical_structure ,Endocrinology ,Internal medicine ,Ascites ,medicine ,Mesenteric lymph nodes ,medicine.symptom - Abstract
Bacterial translocation appears to be an important mechanism in the pathogenesis of spontaneous infections in cirrhosis. Cirrhotic patients are commonly treated with beta-adrenoceptor blockers, but the impact of this treatment in the factors promoting bacterial translocation has not been investigated. This study was aimed at investigating in cirrhotic rats with ascites the effect of propranolol on intestinal bacterial load, transit, and permeability of the bowel and on the rate of bacterial translocation. Bacterial translocation to mesenteric lymph nodes and intestinal bacterial overgrowth, permeability (urinary excretion of (99m)Tc-diethylenetriaminepentaacetic acid [(99m)Tc-DTPA]), and transit (geometric center ratio of (51)Cr) were assessed in 29 rats with carbon tetrachloride (CCl(4)) cirrhosis and 20 controls. These variables were then measured in 12 placebo- and in 13 propranolol-treated ascitic cirrhotic rats. Bacterial translocation was present in 48% of the cirrhotic rats and in none of the controls. Cirrhotic rats with intestinal bacterial overgrowth had a significantly higher rate of translocation and slower intestinal transit than those without it. Among the 15 rats with overgrowth and a (99m)Tc-DTPA excretion greater than 10%, 15 had translocation and 2 had bacterial peritonitis. Only 1 of the 14 rats with either intestinal overgrowth or a (99m)Tc-DTPA excretion greater than 10% presented translocation. Compared with the placebo group, propranolol-treated animals had significantly lower portal pressure, faster intestinal transit, and lower rates of bacterial overgrowth and translocation. In ascitic cirrhotic rats, bacterial translocation results from intestinal overgrowth and severe damage to gut permeability. In this setting, intestinal overgrowth is associated with intestinal hypomotility. Propranolol accelerates the intestinal transit, decreasing the rates of bacterial overgrowth and translocation.
- Published
- 2000
11. Selective impairment of endothelium-mediated vasodilation in liver transplant recipients with cyclosporin A -induced hypertension
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María-Teresa Torres, María Pérez-Páramo, I. Rossi, C. Barrios, Guillermo Cacho, Melchor Alvarez-Mon, Javier Muñoz, Valentín Cuervas-Mons, Juán Gómez-Arnau, José Luis Calleja, Escartin P, Agustín Albillos, and Rosa Daza
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medicine.medical_specialty ,Hepatology ,business.industry ,Vasodilation ,Transplantation ,surgical procedures, operative ,medicine.anatomical_structure ,Endocrinology ,Forearm ,medicine.artery ,Cyclosporin a ,Internal medicine ,Cardiology ,medicine ,Methacholine ,Sodium nitroprusside ,Brachial artery ,business ,Phenylephrine ,medicine.drug - Abstract
Arterial hypertension is commonly observed in orthotopic liver transplantation (OLT) recipients receiving cyclosporin A (CsA), but the precise pathogenetic mechanisms remain partially unknown. The aim of this study was to investigate endothelium-dependent and -independent dilation and adrenergic constriction of resistance vessels of OLT recipients treated with CsA. Vascular reactivity was examined in 22 OLT patients, 10 with and 12 without arterial hypertension, and in 10 control subjects by assessing the forearm blood flow response to the brachial artery infusion of increasing concentrations of methacholine chloride, sodium nitroprusside, and phenylephrine. In 10 OLT patients, the response to methacholine was also examined after acetylsalicylate. The ratio of serum nitrite and nitrate to serum creatinine was lower (P
- Published
- 1998
12. Right ventricular systolic time intervals determined by means of a pulmonary artery catheter
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Ricardo Valero, Braulio De La Calle, Javier Muñoz, Maria T. Alberca, and J. E. Guerrero
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Male ,medicine.medical_specialty ,Time Factors ,animal structures ,Systole ,Critical Illness ,medicine.medical_treatment ,Thermodilution ,Critical Care and Intensive Care Medicine ,Internal medicine ,medicine.artery ,medicine ,Humans ,Prospective Studies ,cardiovascular diseases ,Prospective cohort study ,Aged ,Monitoring, Physiologic ,Ejection fraction ,medicine.diagnostic_test ,business.industry ,Hemodynamics ,Pulmonary artery catheter ,Reproducibility of Results ,Stroke Volume ,Middle Aged ,Intensive Care Units ,Preload ,Blood pressure ,medicine.anatomical_structure ,Ventricle ,Catheterization, Swan-Ganz ,Pulmonary artery ,Linear Models ,Ventricular Function, Right ,cardiovascular system ,Cardiology ,Female ,business ,Electrocardiography - Abstract
OBJECTIVES To evaluate the right ventricular systolic time interval as an index of right ventricular function and also to ascertain whether the right ventricular ejection fraction may be determined by means of a conventional pulmonary artery catheter. DESIGN Prospective study. SETTING Intensive care unit. PATIENTS Eight, consecutive critically ill adult patients. METHODS Simultaneous blind measurements, performed by two investigators, of the right ventricular systolic time interval and right ventricular ejection fraction, determined by means of a pulmonary artery catheter. Two studies, separated by an interval of 24 hrs, per patient. Linear regression analysis. Multiple regression test. RESULTS Of the 16 studies performed, two determinations of right ventricular systolic time intervals were technically inadequate. In the remaining 14 valid studies, we found one close linear correlation between the right ventricular ejection fraction and the preejection period/ejection time quotient measured using the simultaneous display of the electrocardiogram (EKG) and pulmonary arterial pressure curve (r2 = .90, p < .001, right ventricular ejection fraction = 68.96-60.59 x [right ventricular preejection period/right ventricular ejection time]). The method proved to be simple, very accurate, with little interobserver variation (8.09 +/- 10.6% interobserver variation for right ventricular preejection period/right ventricular ejection time) and provided adequate information regarding situations in which the performance of the right ventricle is modified in a given patient. The right ventricular preejection period/right ventricular ejection time quotient was the only variable that displayed a significant relationship with the right ventricular ejection fraction in the multivariate analysis (p < .001). CONCLUSIONS Right ventricular systolic time intervals, measured using the simultaneous display of the pulmonary artery catheter curve and EKG, provide adequate information regarding right ventricle performance in critically ill patients. The close linear correlation between the right ventricular preejection period/right ventricular ejection time quotient and the right ventricular ejection fraction enables the investigator to estimate, with a high degree of accuracy, the right ventricular ejection fraction and the values derived from the preload of the right ventricle, without the need for a modified pulmonary artery catheter.
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- 1992
13. 614
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Olivencia, Lorena, primary, Fernández, Maria Dolores, additional, Macias, Ines, additional, bono, Javier Muñoz, additional, Alvarez, Miguel, additional, Gutierrez, Raquel, additional, Trujillo, Elena, additional, and Rivera, Ricardo, additional
- Published
- 2013
- Full Text
- View/download PDF
14. [Untitled]
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Javier Muñoz Bono, Elena Trujillo, Miguel A. Alvarez, María Dolores Lanzarote Fernández, Lorena Olivencia, Ines Macias, Ricardo Rivera, and Raquel Gutierrez
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medicine.medical_specialty ,Valve replacement ,business.industry ,medicine.medical_treatment ,medicine ,Critical Care and Intensive Care Medicine ,business ,Prosthesis ,Surgery - Published
- 2013
15. 843
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Perez, Gabino Jimenez, primary, Balsera, Emilio Curiel, additional, Bono, Javier Muñoz, additional, and Fernandez, Ricardo Rivera, additional
- Published
- 2012
- Full Text
- View/download PDF
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