1. Immunophenotype of Vestibular Schwannomas
- Author
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Yu Mei, Ian F. Dunn, Sally Al Abdulmohsen, Saksham Gupta, Wenya Linda Bi, Alexandra M Giantini Larsen, Malak Abedalthagafi, Prashin Unadkat, and Shakti H. Ramkissoon
- Subjects
Pathology ,medicine.medical_specialty ,T cell ,Population ,Variable Expression ,03 medical and health sciences ,0302 clinical medicine ,Immunophenotyping ,Immune system ,Biomarkers, Tumor ,Tumor Microenvironment ,otorhinolaryngologic diseases ,medicine ,Humans ,030223 otorhinolaryngology ,education ,education.field_of_study ,Cluster of differentiation ,business.industry ,CD68 ,Macrophages ,Neuroma, Acoustic ,Sensory Systems ,Tumor Burden ,medicine.anatomical_structure ,Otorhinolaryngology ,Neurology (clinical) ,Neoplasm Recurrence, Local ,business ,030217 neurology & neurosurgery ,CD8 - Abstract
BACKGROUND Vestibular schwannomas exhibit a uniquely variable natural history of growth, stability, or even spontaneous regression. We hypothesized that a transitory population of immune cells, or immunomodulation of tumors cells, may influence the growth pattern of schwannomas. We therefore sought to characterize the impact of the immune microenvironment on schwannoma behavior. METHODS Forty-eight vestibular schwannomas with preoperative magnetic resonance imaging and 11 with serial imaging were evaluated for presence of immune infiltrates (including the pan-leukocyte marker Cluster of Differentiation (CD)45, CD4 and CD8 T-cell, and CD68 and CD163 macrophages) as well as expression of immunomodulatory regulators (Programmed Death Ligand 1 (PD-L1), Programmed Death Ligand 2 (PD-L2), LAG-3, TIM-3, V-domain Ig Suppressor of T cell Activation). Maximal diameter, volume, and recurrence were annotated. RESULTS Vestibular schwannomas were characterized by diverse signatures of tumor infiltrating leukocytes and immunomodulatory markers. The median tumor volume was 4.7 cm (Interquartile Range (IQR) 1.0-13.0) and maximum diameter was 2.3 cm (IQR 1.5-3.2). Among tumors with serial imaging, the median volumetric growth was 0.04 cm/mo (IQR 0.01-0.18). Tumor volume and maximum diameter demonstrated strong concordance (R = 0.90; p
- Published
- 2020