1. The Effects of (-)-Epigallocatechin-3-Galate on Wound Closure and Infections in Mice
- Author
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S.H. Hyon, George F. Babcock, Andrew R. Osterburg, Osterburg Ar, Chad T. Robinson, and Ryo Yamaguchi
- Subjects
Materials science ,Contraction (grammar) ,In vitro toxicology ,food and beverages ,Pharmacology ,Antimicrobial ,complex mixtures ,In vitro ,medicine.anatomical_structure ,Dermis ,In vivo ,Immunology ,medicine ,sense organs ,Wound healing ,Myofibroblast - Abstract
In this study, we investigated the effects of (-)-epigallocatechin-3-gallate (EGCG) on wound healing both in vitro and in vivo with and without infection. EGCG has antimicrobial properties and could be useful as a topical agent to prevent and/or treat wound infections. Normal fibroblasts were isolated from the dermis of C57BL/6 mice and cultured with 0, 0.001 to 0.400 mg/ml of EGCG. In vitro assays demonstrated that migration, proliferation, and apop- tosis were inhibited at EGCG concentrations of 0.100-0.400 mg/ml. Expression of α-smooth muscle actin (α-SMA) was also reduced. In vivo experiments measured closure/contraction of full-thickness dorsal wounds that were treated with 0, 0.3, 3.0, and 30.0 mg/ml of EGCG every 24 hours. Macrophages (F4/80), neutrophils (Ly-6G) and myofibroblasts (α-SMA) were assessed at 48 and 168 hours. By 168 hours there was a significant reduction in presence with the 30 mg/ml dose vs. 0.3 and 3.0 mg/ml (P
- Published
- 2015
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