1. [Primary structure of fragment 7 of the influenza virus A/USSR/90/77(H1N1) RNA].
- Author
-
Samokhvalov EI, Karginov VA, Chizhikov VE, Blinov VM, and Iuferov VP
- Subjects
- Amino Acid Sequence, Base Sequence, Influenza A virus genetics, Nucleic Acid Conformation, RNA, Viral genetics, Repetitive Sequences, Nucleic Acid, Viral Proteins analysis, Viral Proteins biosynthesis, Influenza A Virus, H1N1 Subtype, Influenza A virus analysis, RNA, Viral analysis
- Abstract
The double-stranded DNA copy of the matrix protein (M) gene of the influenza virus A/USSR/90/77 (H1N1) has been inserted in PstI site of plasmid pBR322 and cloned in E. coli. The full-length DNA copy of the M gene has been sequenced using Maxam-Gilbert method. Analysis of nucleotide sequence of segment 7 RNA of influenza virus A/USSR/90/77 and nucleotide substitutions, as compared with known primary structures of segment 7 RNA for other strains, is presented. A hypothetical model of secondary structure of segment 7 RNA of influenza virus and repeating sequences at nucleotide and amino acid levels, revealed in the central region of M1 protein, are discussed.
- Published
- 1985