1. A Printed Multicomponent Paper Sensor for Bacterial Detection
- Author
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Carlos D. M. Filipe, Christine L. Brown, Balamurali Kannan, M. Monsur Ali, John D. Brennan, Yingfu Li, and Sana Jahanshahi-Anbuhi
- Subjects
Paper ,Materials science ,Lysis ,lcsh:Medicine ,02 engineering and technology ,Biosensing Techniques ,010402 general chemistry ,01 natural sciences ,Signal ,Article ,Fluorescence ,chemistry.chemical_compound ,Limit of Detection ,lcsh:Science ,Glucans ,Detection limit ,Multidisciplinary ,Chromatography ,Inkwell ,Bacteria ,business.industry ,lcsh:R ,Trehalose ,Pullulan ,DNA, Catalytic ,021001 nanoscience & nanotechnology ,Enzymes, Immobilized ,0104 chemical sciences ,Biotechnology ,chemistry ,Molecular Probes ,lcsh:Q ,Muramidase ,Lysozyme ,0210 nano-technology ,business - Abstract
We present a simple all-in-one paper-based sensor for E. coli detection using a composite ink made of a fluorogenic DNAzyme probe for bacterial recognition and signal generation, lysozyme that lyses whole bacterial cells, and pullulan/trehalose sugars that stabilize printed bioactive molecules. The paper sensor is capable of producing a fluorescence signal as a readout within 5 minutes upon contacting E. coli, can achieve a limit of detection of 100 cells/mL, in a variety of sample matrixes, without sample enrichment, and remains stable for at least 6 months when stored at ambient temperature. Therefore, this simple paper sensor provides rapid bacterial testing on site, and can be shipped and stored under ambient conditions to benefit users living in resource-limited regions.
- Published
- 2017