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Your search keyword '"Wu Yin-Huan"' showing total 2 results
Start Over Author "Wu Yin-Huan" Publisher nature publishing group
2 results on '"Wu Yin-Huan"'

1. Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum.

Author

Liang QY, Wu YH, Wang K, Bai ZY, Liu QL, Pan YZ, Zhang L, and Jiang BB

Subjects
CYS2-HIS2 Zinc Fingers, Catalase genetics, Catalase metabolism, Chrysanthemum drug effects, Chrysanthemum metabolism, Hydrogen Peroxide metabolism, Malondialdehyde metabolism, Peroxidase genetics, Peroxidase metabolism, Plant Leaves drug effects, Plant Leaves genetics, Plant Leaves metabolism, Plant Proteins metabolism, Plant Roots drug effects, Plant Roots genetics, Plant Roots metabolism, Plant Stems drug effects, Plant Stems genetics, Plant Stems metabolism, Plants, Genetically Modified, Protein Domains, Seedlings drug effects, Seedlings genetics, Seedlings metabolism, Sodium Chloride pharmacology, Superoxide Dismutase genetics, Superoxide Dismutase metabolism, Superoxides metabolism, Transcription Factors metabolism, Chrysanthemum genetics, Gene Expression Regulation, Plant, Plant Proteins genetics, Salt Stress genetics, Salt Tolerance genetics, Transcription Factors genetics
Abstract

WRKY transcription factors play important roles in plant growth development, resistance and substance metabolism regulation. However, the exact function of the response to salt stress in plants with specific WRKY transcription factors remains unclear. In this research, we isolated a new WRKY transcription factor DgWRKY5 from chrysanthemum. DgWRKY5 contains two WRKY domains of WKKYGQK and two C 2 H 2 zinc fingers. The expression of DgWRKY5 in chrysanthemum was up-regulated under various treatments. Meanwhile, we observed higher expression levels in the leaves contrasted with other tissues. Under salt stress, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzymes in transgenic chrysanthemum were significantly higher than those in WT, whereas the accumulation of H 2 O 2 , O 2 - and malondialdehyde (MDA) was reduced in transgenic chrysanthemum. Several parameters including root length, root length, fresh weight, chlorophyll content and leaf gas exchange parameters in transgenic chrysanthemum were much better compared with WT under salt stress. Moreover, the expression of stress-related genes DgAPX, DgCAT, DgNCED3A, DgNCED3B, DgCuZnSOD, DgP5CS, DgCSD1 and DgCSD2 was up-regulated in DgWRKY5 transgenic chrysanthemum compared with that in WT. These results suggested that DgWRKY5 could function as a positive regulator of salt stress in chrysanthemum.

Published
2017
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2. Whole-transcriptome sequence analysis of differentially expressed genes in Phormium tenax under drought stress.

Author

Bai ZY, Wang T, Wu YH, Wang K, Liang QY, Pan YZ, Jiang BB, Zhang L, Liu GL, Jia Y, and Liu QL

Subjects
Plant Proteins genetics, Plant Proteins metabolism, Asphodelaceae physiology, Droughts, Stress, Physiological, Transcriptome, Asphodelaceae genetics
Abstract

Phormium tenax is a kind of drought resistant garden plant with its rich and colorful leaves. To clarify the molecular mechanism of drought resistance in Phormium tenax, transcriptome was sequenced by the Illumina sequencing technology under normal and drought stress, respectively. A large number of contigs, transcripts and unigenes were obtained. Among them, only 30,814 unigenes were annotated by comparing with the protein databases. A total of 4,380 genes were differentially expressed, 2,698 of which were finally annotated under drought stress. Differentially expression analysis was also performed upon drought treatment. In KEGG pathway, the mechanism of drought resistance in Phormium tenax was explained from three aspects of metabolism and signaling of hormones, osmotic adjustment and reactive oxygen species metabolism. These results are helpful to understand the drought tolerance mechanism of Phormium tenax and will provide a precious genetic resource for drought-resistant vegetation breeding and research., Competing Interests: The authors declare no competing financial interests.

Published
2017
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