10 results on '"Siemens, Jan"'
Search Results
2. Mutations in cadherin 23 affect tip links in zebrafish sensory hair cells
- Author
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Sollner, Christian, Rauch, Gerd-Jorg, Siemens, Jan, Geisler, Robert, Schuster, Stephan C., Tubingen 2000 Screen Consortium, the, Muller, Ulrich, and Nicolson, Teresa
- Subjects
Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Author(s): Christian Söllner [1]; Gerd-Jörg Rauch [2]; Jan Siemens [4]; Robert Geisler [2]; Stephan C. Schuster [3]; the Tübingen 2000 Screen Consortium [6]; Ulrich Müller [4]; Teresa Nicolson (corresponding author) [...]
- Published
- 2004
- Full Text
- View/download PDF
3. Cadherin 23 is a component of the tip link in hair-cell stereocilia
- Author
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Siemens, Jan, Lillo, Concepcion, Dumont, Rachel A., Reynolds, Anna, Williams, David S., Gillespie, Peter G., and Muller, Ulrich
- Subjects
Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Author(s): Jan Siemens [1]; Concepcion Lillo [2]; Rachel A. Dumont [3]; Anna Reynolds [1]; David S. Williams [2]; Peter G. Gillespie [3]; Ulrich Müller (corresponding author) [1] Mechanoelectrical transduction, the [...]
- Published
- 2004
- Full Text
- View/download PDF
4. Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1
- Author
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Rathjen, Thomas, Yan, Xin, Kononenko, Natalia L., Ku, Min-Chi, Song, Kun, Ferrarese, Leiron, Tarallo, Valentina, Puchkov, Dmytro, Kochlamazashvili, Gaga, Brachs, Sebastian, Varela, Luis, Szigeti-Buck, Klara, Yi, Chun-Xia, Schriever, Sonja C., Tattikota, Sudhir Gopal, Carlo, Anne Sophie, Moroni, Mirko, Siemens, Jan, Heuser, Arnd, van der Weyden, Louise, Birkenfeld, Andreas L., Niendorf, Thoralf, Poulet, James F. A., Horvath, Tamas L., Tschop, Matthias H., Heinig, Matthias, Trajkovski, Mirko, Haucke, Volker, Poy, Matthew N., Rathjen, Thomas, Yan, Xin, Kononenko, Natalia L., Ku, Min-Chi, Song, Kun, Ferrarese, Leiron, Tarallo, Valentina, Puchkov, Dmytro, Kochlamazashvili, Gaga, Brachs, Sebastian, Varela, Luis, Szigeti-Buck, Klara, Yi, Chun-Xia, Schriever, Sonja C., Tattikota, Sudhir Gopal, Carlo, Anne Sophie, Moroni, Mirko, Siemens, Jan, Heuser, Arnd, van der Weyden, Louise, Birkenfeld, Andreas L., Niendorf, Thoralf, Poulet, James F. A., Horvath, Tamas L., Tschop, Matthias H., Heinig, Matthias, Trajkovski, Mirko, Haucke, Volker, and Poy, Matthew N.
- Abstract
Susceptibility to obesity is linked to genes regulating neurotransmission, pancreatic beta-cell function and energy homeostasis. Genome-wide association studies have identified associations between body mass index and two loci near cell adhesion molecule 1 (CADM1) and cell adhesion molecule 2 (CADM2), which encode membrane proteins that mediate synaptic assembly. We found that these respective risk variants associate with increased CADM1 and CADM2 expression in the hypothalamus of human subjects. Expression of both genes was elevated in obese mice, and induction of Cadm1 in excitatory neurons facilitated weight gain while exacerbating energy expenditure. Loss of Cadm1 protected mice from obesity, and tract-tracing analysis revealed Cadm1-positive innervation of POMC neurons via afferent projections originating from beyond the arcuate nucleus. Reducing Cadm1 expression in the hypothalamus and hippocampus promoted a negative energy balance and weight loss. These data identify essential roles for Cadm1-mediated neuronal input in weight regulation and provide insight into the central pathways contributing to human obesity.
- Published
- 2017
5. The menthol receptor TRPM8 is the principal detector of environmental cold
- Author
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Bautista, Diana M., Siemens, Jan, Glazer, Joshua M., Tsuruda, Pamela R., Basbaum, Allan I., Stucky, Cheryl L., Jordt, Sven-Eric, and Julius, David
- Subjects
Ion channels -- Research -- Physiological aspects -- Usage ,Sensory receptors -- Research -- Physiological aspects -- Usage ,Environmental issues ,Science and technology ,Zoology and wildlife conservation ,Physiological aspects ,Usage ,Research - Abstract
Author(s): Diana M. Bautista [1, 2, 7]; Jan Siemens [1, 2, 7]; Joshua M. Glazer [5, 7]; Pamela R. Tsuruda [1, 2]; Allan I. Basbaum [3, 4]; Cheryl L. Stucky [...]
- Published
- 2007
- Full Text
- View/download PDF
6. Astrocytes mediate the effect of oxytocin in the central amygdala on neuronal activity and affective states in rodents.
- Author
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Wahis J, Baudon A, Althammer F, Kerspern D, Goyon S, Hagiwara D, Lefevre A, Barteczko L, Boury-Jamot B, Bellanger B, Abatis M, Da Silva Gouveia M, Benusiglio D, Eliava M, Rozov A, Weinsanto I, Knobloch-Bollmann HS, Kirchner MK, Roy RK, Wang H, Pertin M, Inquimbert P, Pitzer C, Siemens J, Goumon Y, Boutrel B, Lamy CM, Decosterd I, Chatton JY, Rouach N, Young WS, Stern JE, Poisbeau P, Stoop R, Darbon P, Grinevich V, and Charlet A
- Subjects
- Animals, Astrocytes drug effects, Behavior, Animal drug effects, Behavior, Animal physiology, Central Amygdaloid Nucleus drug effects, Female, Male, Mice, Mice, Inbred C57BL, Oxytocin pharmacology, Rats, Rats, Wistar, Receptors, Oxytocin metabolism, Astrocytes metabolism, Central Amygdaloid Nucleus metabolism, Emotions physiology, Neurons metabolism, Oxytocin metabolism
- Abstract
Oxytocin (OT) orchestrates social and emotional behaviors through modulation of neural circuits. In the central amygdala, the release of OT modulates inhibitory circuits and, thereby, suppresses fear responses and decreases anxiety levels. Using astrocyte-specific gain and loss of function and pharmacological approaches, we demonstrate that a morphologically distinct subpopulation of astrocytes expresses OT receptors and mediates anxiolytic and positive reinforcement effects of OT in the central amygdala of mice and rats. The involvement of astrocytes in OT signaling challenges the long-held dogma that OT acts exclusively on neurons and highlights astrocytes as essential components for modulation of emotional states under normal and chronic pain conditions.
- Published
- 2021
- Full Text
- View/download PDF
7. Smectite clay minerals reduce the acute toxicity of quaternary alkylammonium compounds towards potentially pathogenic bacterial taxa present in manure and soil.
- Author
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Heyde BJ, Glaeser SP, Bisping L, Kirchberg K, Ellinghaus R, Siemens J, and Mulder I
- Subjects
- Adsorption, Bacteria, Clay, Manure microbiology, Minerals, Silicates chemistry, Soil chemistry, Soil Microbiology, Surface-Active Agents, Quaternary Ammonium Compounds toxicity, Silicates pharmacology
- Abstract
Quaternary alkylammonium compounds (QAACs) are a group of cationic surfactants which are disinfectants with numerous industrial and agricultural applications and frequently released into the environment. One recent hypothesis is that bacteria present in soil will be protected from acute toxic effects of QAACs in the presence of expandable layer silicates due to interlayer sorption. We therefore studied bacterial growth kinetics with high temporal resolution and determined minimal inhibitory concentrations (MICs) of two QAACs, benzyldimethyldodecylammonium chloride (BAC-C12) and didecyldimethylammonium chlorid (DADMAC-C10), for eight strains of different bacterial taxa (Escherichia coli, Acinetobacter, Enterococcus faecium, Enterococcus faecalis, and Pseudomonas fluorescens) in relation to QAAC sorption to smectite and kaolinite. The MICs of BAC-C12 and DADMAC-C10 were in the absence of smectite and kaolinite in the order of 10 to 30 µg mL
-1 and 1.0 to 3.5 µg mL-1 for all strains except the more sensitive Acinetobacter strain. For all tested strains and both tested QAACs, the presence of smectite increased apparent MIC values while kaolinite had no effect on MICs. Sorption curves without bacteria showed that smectite sorbed larger amounts of QAACs than kaolinite. Correcting nominal QAAC concentrations employed in toxicity tests for QAAC sorption using the sorption curves explained well the observed shifts in apparent MICs. Transmission electron microscopy (TEM) demonstrated that the interlayer space of smectite expanded from 13.7 ± 1 Å to 19.9 ± 1.5 Å after addition of BAC-C12. This study provides first evidence that low charge 2:1 expandable layer silicates can play an important role for buffering QAAC toxicity in soils.- Published
- 2020
- Full Text
- View/download PDF
8. Regulation of body weight and energy homeostasis by neuronal cell adhesion molecule 1.
- Author
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Rathjen T, Yan X, Kononenko NL, Ku MC, Song K, Ferrarese L, Tarallo V, Puchkov D, Kochlamazashvili G, Brachs S, Varela L, Szigeti-Buck K, Yi CX, Schriever SC, Tattikota SG, Carlo AS, Moroni M, Siemens J, Heuser A, van der Weyden L, Birkenfeld AL, Niendorf T, Poulet JFA, Horvath TL, Tschöp MH, Heinig M, Trajkovski M, Haucke V, and Poy MN
- Subjects
- Animals, Cell Adhesion Molecule-1, Energy Metabolism physiology, Genome-Wide Association Study, Homeostasis physiology, Membrane Proteins metabolism, Mice, Transgenic, Neurons metabolism, Obesity genetics, Pro-Opiomelanocortin metabolism, Arcuate Nucleus of Hypothalamus metabolism, Body Weight physiology, Cell Adhesion Molecules genetics, Cell Adhesion Molecules, Neuronal genetics, Homeostasis genetics, Immunoglobulins genetics, Obesity metabolism
- Abstract
Susceptibility to obesity is linked to genes regulating neurotransmission, pancreatic beta-cell function and energy homeostasis. Genome-wide association studies have identified associations between body mass index and two loci near cell adhesion molecule 1 (CADM1) and cell adhesion molecule 2 (CADM2), which encode membrane proteins that mediate synaptic assembly. We found that these respective risk variants associate with increased CADM1 and CADM2 expression in the hypothalamus of human subjects. Expression of both genes was elevated in obese mice, and induction of Cadm1 in excitatory neurons facilitated weight gain while exacerbating energy expenditure. Loss of Cadm1 protected mice from obesity, and tract-tracing analysis revealed Cadm1-positive innervation of POMC neurons via afferent projections originating from beyond the arcuate nucleus. Reducing Cadm1 expression in the hypothalamus and hippocampus promoted a negative energy balance and weight loss. These data identify essential roles for Cadm1-mediated neuronal input in weight regulation and provide insight into the central pathways contributing to human obesity.
- Published
- 2017
- Full Text
- View/download PDF
9. PIEZO2 is required for mechanotransduction in human stem cell-derived touch receptors.
- Author
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Schrenk-Siemens K, Wende H, Prato V, Song K, Rostock C, Loewer A, Utikal J, Lewin GR, Lechner SG, and Siemens J
- Subjects
- Animals, Basic Helix-Loop-Helix Transcription Factors physiology, Cell Differentiation physiology, Female, Humans, Ion Channels genetics, Mice, Mice, Inbred C57BL, Mice, Knockout, Nerve Tissue Proteins physiology, Neural Crest cytology, Induced Pluripotent Stem Cells physiology, Ion Channels physiology, Mechanoreceptors physiology, Mechanotransduction, Cellular physiology, Sensory Receptor Cells physiology, Touch physiology
- Abstract
Human sensory neurons are inaccessible for functional examination, and thus little is known about the mechanisms mediating touch sensation in humans. Here we demonstrate that the mechanosensitivity of human embryonic stem (hES) cell-derived touch receptors depends on PIEZO2. To recapitulate sensory neuron development in vitro, we established a multistep differentiation protocol and generated sensory neurons via the intermediate production of neural crest cells derived from hES cells or human induced pluripotent stem (hiPS) cells. The generated neurons express a distinct set of touch receptor-specific genes and convert mechanical stimuli into electrical signals, their most salient characteristic in vivo. Strikingly, mechanosensitivity is lost after CRISPR/Cas9-mediated PIEZO2 gene deletion. Our work establishes a model system that resembles human touch receptors, which may facilitate mechanistic analysis of other sensory subtypes and provide insight into developmental programs underlying sensory neuron diversity.
- Published
- 2015
- Full Text
- View/download PDF
10. A somatosensory circuit for cooling perception in mice.
- Author
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Milenkovic N, Zhao WJ, Walcher J, Albert T, Siemens J, Lewin GR, and Poulet JF
- Subjects
- Animals, Cold Temperature, Male, Mice, Inbred C57BL, Mice, Knockout, Physical Stimulation methods, Skin innervation, Skin Physiological Phenomena, TRPM Cation Channels metabolism, Touch physiology, Neurons physiology, Perception physiology, Thermosensing physiology
- Abstract
The temperature of an object provides important somatosensory information for animals performing tactile tasks. Humans can perceive skin cooling of less than one degree, but the sensory afferents and central circuits that they engage to enable the perception of surface temperature are poorly understood. To address these questions, we examined the perception of glabrous skin cooling in mice. We found that mice were also capable of perceiving small amplitude skin cooling and that primary somatosensory (S1) cortical neurons were required for cooling perception. Moreover, the absence of the menthol-gated transient receptor potential melastatin 8 ion channel in sensory afferent fibers eliminated the ability to perceive cold and the corresponding activation of S1 neurons. Our results identify parts of a neural circuit underlying cold perception in mice and provide a new model system for the analysis of thermal processing and perception and multimodal integration.
- Published
- 2014
- Full Text
- View/download PDF
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