1. Loss of Stemness, EMT, and Supernumerary Tooth Formation in Cebpb −/− Runx2 +/− Murine Incisors
- Author
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Katsu Takahashi, Masaki Nagata, Toshihisa Komori, Honoka Kiso, Masato Asai, Akira Shimizu, Yoshihito Tokita, Sayaka Mishima, Mary MacDougall, Hiroko Tsukamoto, Kazuhisa Bessho, Manabu Sugai, Boyen Huang, Machiko Iida, Yumiko Togo, Kazuyuki Saito, Masakazu Asahara, and Hidemitsu Harada
- Subjects
0301 basic medicine ,Multidisciplinary ,Enamel paint ,Biglycan ,lcsh:R ,Cervical loop ,lcsh:Medicine ,Biology ,Cell biology ,03 medical and health sciences ,stomatognathic diseases ,030104 developmental biology ,medicine.anatomical_structure ,SOX2 ,stomatognathic system ,visual_art ,embryonic structures ,visual_art.visual_art_medium ,CEBPB ,Dentin ,medicine ,lcsh:Q ,Stem cell ,Ameloblast ,lcsh:Science - Abstract
Adult Cebpb KO mice incisors present amelogenin-positive epithelium pearls, enamel and dentin allopathic hyperplasia, fewer Sox2-positive cells in labial cervical loop epitheliums, and reduced Sox2 expression in enamel epithelial stem cells. Thus, Cebpb acts upstream of Sox2 to regulate stemness. In this study, Cebpb KO mice demonstrated cementum-like hard tissue in dental pulp, loss of polarity by ameloblasts, enamel matrix in ameloblastic layer, and increased expression of epithelial-mesenchymal transition (EMT) markers in a Cebpb knockdown mouse enamel epithelial stem cell line. Runx2 knockdown in the cell line presented a similar expression pattern. Therefore, the EMT enabled disengaged odontogenic epithelial stem cells to develop supernumerary teeth. Cebpb and Runx2 knockdown in the cell line revealed higher Biglycan and Decorin expression, and Decorin-positive staining in the periapical region, indicating their involvement in supernumerary tooth formation. Cebpb and Runx2 acted synergistically and played an important role in the formation of supernumerary teeth in adult incisors.
- Published
- 2018