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Your search keyword '"Luigi Ciuffreda"' showing total 2 results
Start Over Author "Luigi Ciuffreda" Publisher nature publishing group
2 results on '"Luigi Ciuffreda"'

1. Combined analysis of miR-200 family and its significance for breast cancer

Author

Maria Morritti, Valentina Melocchi, Andrea Fontana, Marina Castelvetere, Paolo Graziano, Sara Ravaioli, Raffaela Barbano, Vanna Maria Valori, Michelina Rendina, Massimiliano Copetti, Elisa Dama, Barbara Pasculli, Sara Bravaccini, Vito Michele Fazio, Paola Parrella, Luigi Ciuffreda, Evaristo Maiello, Manel Esteller, and Fabrizio Bianchi

Subjects
0301 basic medicine, Oncology, Molecular biology, Cancer specific survival, Cohort Studies, 0302 clinical medicine, Breast cancer, Breast, skin and connective tissue diseases, Triple negative, Cancer, Multidisciplinary, Biochemical markers, Middle Aged, Up-Regulation, Gene Expression Regulation, Neoplastic, 030220 oncology & carcinogenesis, Multigene Family, Cohort, Marcadors bioquímics, Medicine, Female, Normal breast, medicine.medical_specialty, Epithelial-Mesenchymal Transition, Science, Breast Neoplasms, Risk Assessment, Article, Disease-Free Survival, Càncer de mama, 03 medical and health sciences, Downregulation and upregulation, Predictive Value of Tests, Internal medicine, Cell Line, Tumor, microRNA, medicine, Biomarkers, Tumor, Humans, Neoplasm Staging, business.industry, Luminal b, medicine.disease, MicroRNAs, 030104 developmental biology, Neoplasm Recurrence, Local, business
Abstract

Fundació Carreras This work was supported by Italian Ministry of Health (MoH) TRANSCAN Joint Transnational Call (JTC) 2013 co-funded by the European Regional Development Fund, "A way of making Europe" RRC-2014-2354565, Italian Ministry of Health (MoH) "Ricerca Corrente 2019" and "5×1000″ voluntary contributions" and partially supported by Associazione Italiana Ricerca sul Cancro (AIRC) Project Code: 16747. While the molecular functions of miR-200 family have been deeply investigated, a role for these miRNAs as breast cancer biomarkers remains largely unexplored. In the attempt to clarify this, we profiled the miR-200 family members expression in a large cohort of breast cancer cases with a long follow-up (H-CSS cohort) and in TCGA-BRCA cohort. Overall, miR-200 family was found upregulated in breast tumors with respect to normal breast tissues while downregulated in more aggressive breast cancer molecular subtypes (i.e. Luminal B, HER2 and triple negative), consistently with their function as repressors of the epithelial-to-mesenchymal transition (EMT). In particular miR-141-3p was found differentially expressed in breast cancer molecular subtypes in both H-CSS and TCGA-BRCA cohorts, and the combined analysis of all miR-200 family members demonstrated a slight predictive accuracy on H-CSS cancer specific survival at 12 years (survival c-statistic: 0.646; 95%CI 0.538-0.754).

Published
2021

2. Stepwise analysis of MIR9 loci identifies miR-9-5p to be involved in Oestrogen regulated pathways in breast cancer patients.

Author

Barbano R, Pasculli B, Rendina M, Fontana A, Fusilli C, Copetti M, Castellana S, Valori VM, Morritti M, Graziano P, Luigi C, Coco M, Picardo F, Mazza T, Evron E, Murgo R, Maiello E, Esteller M, Fazio VM, and Parrella P

Subjects
Aged, Biomarkers, Tumor genetics, Female, Gene Expression Profiling methods, Gene Expression Regulation, Neoplastic genetics, Humans, Middle Aged, Promoter Regions, Genetic genetics, Prospective Studies, Breast Neoplasms genetics, Estrogens genetics, Genetic Loci genetics, MicroRNAs genetics, Signal Transduction genetics
Abstract

miR-9 was initially identified as an epigenetically regulated miRNA in tumours, but inconsistent findings have been reported so far. We analysed the expression of miR-9-5p, miR-9-3p, pri-miRs and MIR9 promoters methylation status in 131 breast cancer cases and 12 normal breast tissues (NBTs). The expression of both mature miRs was increased in tumours as compared to NBTs (P < 0.001) and negatively correlated with ER protein expression (P = 0.005 and P = 0.003, for miR-9-3p and miR-9-5p respectively). In addition, miR-9-5p showed a significant negative correlation with PgR (P = 0.002). Consistently, miR-9-5p and miR-9 3p were differentially expressed in the breast cancer subgroups identified by ER and PgR expression and HER2 amplification. No significant correlation between promoter methylation and pri-miRNAs expressions was found either in tumours or in NBTs. In the Luminal breast cancer subtype the expression of miR-9-5p was associated with a worse prognosis in both univariable and multivariable analyses. Ingenuity Pathway Analysis exploring the putative interactions among miR-9-5p/miR-9-3p, ER and PgR upstream and downstream regulators suggested a regulatory loop by which miR-9-5p but not miR-9-3p is induced by steroid hormone receptor and acts within hormone-receptor regulated pathways.

Published
2017
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