1. New Compound Sets Identified from High Throughput Phenotypic Screening Against Three Kinetoplastid Parasites: An Open Resource
- Author
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Imanol Peña, Gonzalo Colmenarejo, Albane Kessler, Irene Roquero, Ignacio Cotillo, Ana I. Bardera, David W. Gray, Vinod Kumar, Emilio Alvarez, Alexander Sherstnev, Julio Alonso-Padilla, Ana Rodriguez, Miguel Navarro, Juan Cantizani, Vanessa Barroso, Francisco de Dios-Anton, James R. Brown, M. Pilar Manzano, Jose M. Fiandor, Julio Martin, and David H. Drewry
- Subjects
Phenotypic screening ,High-throughput screening ,Antiprotozoal Agents ,Drug Evaluation, Preclinical ,Computational biology ,Biology ,Trypanosoma brucei ,Bioinformatics ,Genome ,Article ,Cell Line ,Small Molecule Libraries ,Mice ,Parasitic Sensitivity Tests ,High-Throughput Screening Assays ,parasitic diseases ,Animals ,Humans ,Kinetoplastida ,Trypanosoma cruzi ,Phylogeny ,Multidisciplinary ,biology.organism_classification ,Biological target ,Genome, Protozoan - Abstract
Using whole-cell phenotypic assays, the GlaxoSmithKline high-throughput screening (HTS) diversity set of 1.8 million compounds was screened against the three kinetoplastids most relevant to human disease, i.e. Leishmania donovani, Trypanosoma cruzi and Trypanosoma brucei. Secondary confirmatory and orthogonal intracellular anti-parasiticidal assays were conducted, and the potential for non-specific cytotoxicity determined. Hit compounds were chemically clustered and triaged for desirable physicochemical properties. The hypothetical biological target space covered by these diversity sets was investigated through bioinformatics methodologies. Consequently, three anti-kinetoplastid chemical boxes of ~200 compounds each were assembled. Functional analyses of these compounds suggest a wide array of potential modes of action against kinetoplastid kinases, proteases and cytochromes as well as potential host–pathogen targets. This is the first published parallel high throughput screening of a pharma compound collection against kinetoplastids. The compound sets are provided as an open resource for future lead discovery programs, and to address important research questions., The support and funding of Tres Cantos Open Lab Foundation is gratefully acknowledged
- Published
- 2015