1. Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris.
- Author
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Krainer FW, Gmeiner C, Neutsch L, Windwarder M, Pletzenauer R, Herwig C, Altmann F, Glieder A, and Spadiut O
- Subjects
- Batch Cell Culture Techniques, Bioreactors, Cell Division genetics, Chromatography, Liquid, Enzyme Activation, Gene Order, Gene Targeting, Glycoproteins chemistry, Mannose-Binding Lectins metabolism, Mannosyltransferases chemistry, Mannosyltransferases isolation & purification, Mannosyltransferases metabolism, Mass Spectrometry, Phenotype, Pichia growth & development, Polysaccharides chemistry, Polysaccharides metabolism, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Stress, Physiological, Fungal Proteins genetics, Fungal Proteins metabolism, Gene Knockout Techniques, Glycoproteins metabolism, Mannosyltransferases genetics, Pichia genetics, Pichia metabolism
- Abstract
The yeast Pichia pastoris is a common host for the recombinant production of biopharmaceuticals, capable of performing posttranslational modifications like glycosylation of secreted proteins. However, the activity of the OCH1 encoded α-1,6-mannosyltransferase triggers hypermannosylation of secreted proteins at great heterogeneity, considerably hampering downstream processing and reproducibility. Horseradish peroxidases are versatile enzymes with applications in diagnostics, bioremediation and cancer treatment. Despite the importance of these enzymes, they are still isolated from plant at low yields with different biochemical properties. Here we show the production of homogeneous glycoprotein species of recombinant horseradish peroxidase by using a P. pastoris platform strain in which OCH1 was deleted. This och1 knockout strain showed a growth impaired phenotype and considerable rearrangements of cell wall components, but nevertheless secreted more homogeneously glycosylated protein carrying mainly Man8 instead of Man10 N-glycans as a dominant core glycan structure at a volumetric productivity of 70% of the wildtype strain.
- Published
- 2013
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