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Start Over Author "Dong Soo Kyung" Publisher nature publishing group
2 results on '"Dong Soo Kyung"'

1. Single-cell transcriptomic analysis reveals dynamic activation of cellular signaling pathways regulating beige adipogenesis

Author

Dong Soo Kyung, Eunmin Lee, Sehyun Chae, Yeonho Son, Ye-Jin Moon, Daehee Hwang, Jong Kyoung Kim, Yun-Hee Lee, and Je Kyung Seong

Subjects
Medicine, Biochemistry, QD415-436
Abstract

Abstract PDGFRA+ cells have been identified as adipocyte stem cells (ASCs) that differentiate into beige adipocytes in white adipose tissue (WAT) following thermogenic stimuli. To elucidate the molecular heterogeneity of ASCs, we conducted single-cell transcriptomic profiling of PDGFRA+ cells isolated from the inguinal WAT (iWAT) of mice treated with the beta3 adrenergic receptor agonist CL316243. Single-cell RNA-seq revealed nine major clusters, which were categorized into four groups: resting, proliferating, differentiating, and adipogenic factor-expressing cells (AFECs). Trajectory analysis revealed sequential activation of molecular pathways, including the Hedgehog and Notch signaling pathways, during beige adipogenesis. AFECs expressed Dpp4 and did not differentiate into adipocytes in culture or after transplantation. Furthermore, genetic lineage tracing studies indicated that DPP4+ cells did not differentiate into adipocytes in iWAT during CL316243-induced beige adipogenesis. However, high-fat diet feeding led to the recruitment of adipocytes from DPP4+ cells in iWAT. Overall, this study improved our understanding of the dynamic molecular basis of beige adipogenesis and the potential contribution of DPP4+ adipocyte lineages to the pathological expansion of WAT during diet-induced obesity.

Published
2024
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2. Global transcriptome analysis identifies weight regain-induced activation of adaptive immune responses in white adipose tissue of mice

Author

Choi Jh, Sung Hr, Yeonju Kim, Yong Ho Lee, Kim Kd, Je Kyung Seong, Kim Iy, Dong Soo Kyung, and Cho Sy

Subjects
0301 basic medicine, Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Adipose Tissue, White, Medicine (miscellaneous), Adipose tissue, White adipose tissue, Biology, Weight Gain, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, Mice, Immune system, Weight loss, Internal medicine, Adipocyte, medicine, Animals, Gonads, Nutrition and Dietetics, Gene Expression Profiling, Weight Fluctuation, Mice, Inbred C57BL, 030104 developmental biology, Endocrinology, chemistry, Liver, Original Article, medicine.symptom, Weight gain
Abstract

Objective: Studies have indicated that weight regain following weight loss predisposes obese individuals to metabolic disorders; however, the molecular mechanism of this potential adverse effect of weight regain is not fully understood. Here we investigated global transcriptome changes and the immune response in mouse white adipose tissue caused by weight regain. Design: We established a diet switch protocol to compare the effects of weight regain with those of weight gain without precedent weight loss, weight loss maintenance and chow diet. We conducted a time course analysis of global transcriptome changes in gonadal white adipose tissue (gWAT) during the weight fluctuation. Co-expression network analysis was used to identify functional modules associated with the weigh regain phenotype. Immune cell populations in gWAT were characterized by flow-cytometric immunophenotyping. Metabolic phenotypes were monitored by histological analysis of adipose tissue and liver, and blood-chemistry and body weight/composition analyses. Results: In total, 952 genes were differentially expressed in the gWAT in the weight regain vs the weight gain group. Upregulated genes were associated with immune response and leukocyte activation. Co-expression network analysis showed that genes involved in major histocompatibility complex I and II-mediated antigen presentation and T-cell activation function were upregulated. Consistent with the transcriptome analysis results, flow cytometry demonstrated significant increases in subsets of T cells and proinflammatory M1 macrophages in the gWAT in the weight regain as compared to the weight gain group. In addition, upregulation of adaptive immune responses was associated with high incidence of adipocyte death and upregulation of high mobility group box 1, a well-known component of damage-associated molecular patterns. Conclusions: Our global transcriptome analysis identified weight regain-induced activation of adaptive immune responses in mouse white adipose tissue. Results suggest that activation of adipocyte death-associated adaptive immunity in adipose tissue may contribute to unfavorable metabolic effects of weight regain following weight loss.

Published
2018

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