Search

Your search keyword '"Dang, H.-H."' showing total 2 results
Start Over Author "Dang, H.-H." Publisher nature publishing group
2 results on '"Dang, H.-H."'

1. Scientific Reports / Unbiased quantitative proteomics reveals a crucial role of the allergen context for the activation of human dendritic cells

Author

Strasser, L., Dang, H.-H., Schwarz, H., Asam, C., Ferreira, F., Horejs-Hoeck, and Huber, C. G.

Abstract

Worldwide, more than 1 billion people suffer from allergic diseases. However, until now it is not fully understood how certain proteins can induce allergic immune responses, while others cannot. Studies suggest that allergenicity is a process not only determined by properties of the allergen itself but also by costimulatory factors, that are not classically associated with allergic reactions. To investigate the allergenicity of the major birch pollen allergen Bet v 1 and the impact of adjuvants associated with pollen, e.g. lipopolysaccharide (LPS), we performed quantitative proteome analysis to study the activation of monocyte-derived dendritic cells (moDCs). Thus, we treated cells with birch pollen extract (BPE), recombinant Bet v 1, and LPS followed by proteomic profiling via high-performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) using isobaric labelling. Enrichment and pathway analysis revealed the influence of regulated proteins especially in cytokine signalling and dendritic cell activation. We found highly regulated, but differentially expressed proteins after treatment with BPE and LPS, whereas the cellular response to Bet v 1 was limited. Our findings lead to the conclusion that Bet v 1 needs a specific "allergen context" involving cofactors apart from LPS to induce an immune response in human moDCs. P25696 (VLID)2382950

Published
2017

2. Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells.

Author

Strasser L, Dang HH, Schwarz H, Asam C, Ferreira F, Horejs-Hoeck J, and Huber CG

Subjects
Analysis of Variance, Biomarkers, Computational Biology methods, Cytokines metabolism, Cytotoxicity, Immunologic, Gene Ontology, Humans, Hypersensitivity immunology, Hypersensitivity metabolism, Immunophenotyping, Lipopolysaccharides immunology, Molecular Sequence Annotation, NF-kappa B metabolism, Allergens immunology, Dendritic Cells immunology, Dendritic Cells metabolism, Proteome, Proteomics methods
Abstract

Worldwide, more than 1 billion people suffer from allergic diseases. However, until now it is not fully understood how certain proteins can induce allergic immune responses, while others cannot. Studies suggest that allergenicity is a process not only determined by properties of the allergen itself but also by costimulatory factors, that are not classically associated with allergic reactions. To investigate the allergenicity of the major birch pollen allergen Bet v 1 and the impact of adjuvants associated with pollen, e.g. lipopolysaccharide (LPS), we performed quantitative proteome analysis to study the activation of monocyte-derived dendritic cells (moDCs). Thus, we treated cells with birch pollen extract (BPE), recombinant Bet v 1, and LPS followed by proteomic profiling via high-performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) using isobaric labelling. Enrichment and pathway analysis revealed the influence of regulated proteins especially in cytokine signalling and dendritic cell activation. We found highly regulated, but differentially expressed proteins after treatment with BPE and LPS, whereas the cellular response to Bet v 1 was limited. Our findings lead to the conclusion that Bet v 1 needs a specific "allergen context" involving cofactors apart from LPS to induce an immune response in human moDCs.

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results