1. Chitin degradation by Synechococcus WH7803.
- Author
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Capovilla G, Castro KG, Collani S, Kearney SM, Kehoe DM, and Chisholm SW
- Subjects
- Chitin metabolism, Synechococcus genetics, Synechococcus metabolism, Chitinases genetics, Chitinases metabolism
- Abstract
Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type., (© 2023. The Author(s).)
- Published
- 2023
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