1. Vitamin D protects against immobilization-induced muscle atrophy via neural crest-derived cells in mice
- Author
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Satoshi Nakamura, Masaya Nakamura, Morio Matsumoto, Tami Kobayashi, Kana Miyamoto, Yuiko Sato, Hiroyuki Okada, Yosuke Kaneko, Eri Ito, Takeshi Miyamoto, Akihito Oya, Tomoya Soma, and Arihiko Kanaji
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Sarcopenia ,lcsh:Medicine ,030209 endocrinology & metabolism ,Calcitriol receptor ,vitamin D deficiency ,Article ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Internal medicine ,medicine ,Vitamin D and neurology ,Animals ,Vitamin D ,Receptor ,Muscle, Skeletal ,lcsh:Science ,Mice, Knockout ,Multidisciplinary ,business.industry ,lcsh:R ,Neural crest ,Endocrine system and metabolic diseases ,medicine.disease ,Vitamin D Deficiency ,Muscle atrophy ,Experimental models of disease ,Mice, Inbred C57BL ,Muscular Atrophy ,030104 developmental biology ,Endocrinology ,Neural Crest ,Receptors, Calcitriol ,Tumor necrosis factor alpha ,Female ,lcsh:Q ,medicine.symptom ,business - Abstract
Vitamin D deficiency is a recognized risk factor for sarcopenia development, but mechanisms underlying this outcome are unclear. Here, we show that low vitamin D status worsens immobilization-induced muscle atrophy in mice. Mice globally lacking vitamin D receptor (VDR) exhibited more severe muscle atrophy following limb immobilization than controls. Moreover, immobilization-induced muscle atrophy was worse in neural crest-specific than in skeletal muscle-specific VDR-deficient mice. Tnfα expression was significantly higher in immobilized muscle of VDR-deficient relative to control mice, and was significantly elevated in neural crest-specific but not muscle-specific VDR-deficient mice. Furthermore, muscle atrophy induced by limb immobilization in low vitamin D mice was significantly inhibited in Tnfα-deficient mice. We conclude that vitamin D antagonizes immobilization-induced muscle atrophy via VDR expressed in neural crest-derived cells.
- Published
- 2020