1. Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells.
- Author
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Carpena NT, Chang SY, Abueva CDG, Jung JY, and Lee MY
- Subjects
- Animals, Biomarkers metabolism, Cell Aggregation drug effects, Cell Line, Coculture Techniques, Culture Media, Conditioned pharmacology, Embryoid Bodies cytology, Embryoid Bodies drug effects, Embryonic Stem Cells drug effects, Embryonic Stem Cells metabolism, Epithelium metabolism, Gene Expression Regulation drug effects, Mice, Myosin VIIa metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, SOXB1 Transcription Factors metabolism, Cell Differentiation drug effects, Cell Differentiation genetics, Embryonic Stem Cells cytology, Hair Cells, Auditory cytology
- Abstract
Several studies have shown how different cell lines can influence the differentiation of stem cells through co-culture systems. The House Ear Institute-Organ of Corti 1 (HEI-OC1) is considered an important cell line for in vitro auditory research. However, it is unknown if HEI-OC1 cells can promote the differentiation of embryonic stem cells (ESCs). In this study, we investigated whether co-culture of ESCs with HEI-OC1 cells promotes differentiation. To this end, we developed a co-culture system of mouse ESCs with HEI-OC1 cells. Dissociated or embryonic bodies (EBs) of ESCs were introduced to a conditioned and inactivated confluent layer of HEI-OC1 cells for 14 days. The dissociated ESCs coalesced into an EB-like form that was smaller than the co-cultured EBs. Contact co-culture generated cells expressing several otic progenitor markers as well as hair cell specific markers. ESCs and EBs were also cultured in non-contact setup but using conditioned medium from HEI-OC1 cells, indicating that soluble factors alone could have a similar effect. The ESCs did not form into aggregates but were still Myo7a-positive, while the EBs degenerated. However, in the fully differentiated EBs, evidence to prove mature differentiation of inner ear hair cell was still rudimentary. Nevertheless, these results suggest that cellular interactions between ESCs and HEI-OC1 cells may both stimulate ESC differentiation.
- Published
- 2021
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