1. Inducible control of gene expression with destabilized Cre.
- Author
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Sando R 3rd, Baumgaertel K, Pieraut S, Torabi-Rander N, Wandless TJ, Mayford M, and Maximov A
- Subjects
- Animals, Humans, Mice, Recombination, Genetic drug effects, Trimethoprim pharmacology, Gene Expression Regulation drug effects, Integrases metabolism
- Abstract
Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research.
- Published
- 2013
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