1. Pneumococcal genome sequencing tracks a vaccine escape variant formed through a multi-fragment recombination event.
- Author
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Golubchik T, Brueggemann AB, Street T, Gertz RE Jr, Spencer CC, Ho T, Giannoulatou E, Link-Gelles R, Harding RM, Beall B, Peto TE, Moore MR, Donnelly P, Crook DW, and Bowden R
- Subjects
- Base Sequence, Computational Biology, DNA Primers genetics, Metagenomics, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, Selection, Genetic, Sequence Analysis, DNA, Species Specificity, United States epidemiology, Genome, Bacterial genetics, Pneumococcal Vaccines genetics, Pneumonia epidemiology, Pneumonia microbiology, Recombination, Genetic genetics, Streptococcus pneumoniae genetics
- Abstract
Streptococcus pneumoniae ('pneumococcus') causes an estimated 14.5 million cases of serious disease and 826,000 deaths annually in children under 5 years of age(1). The highly effective introduction of the PCV7 pneumococcal vaccine in 2000 in the United States(2,3) provided an unprecedented opportunity to investigate the response of an important pathogen to widespread, vaccine-induced selective pressure. Here, we use array-based sequencing of 62 isolates from a US national monitoring program to study five independent instances of vaccine escape recombination(4), showing the simultaneous transfer of multiple and often large (up to at least 44 kb) DNA fragments. We show that one such new strain quickly became established, spreading from east to west across the United States. These observations clarify the roles of recombination and selection in the population genomics of pneumococcus and provide proof of principle of the considerable value of combining genomic and epidemiological information in the surveillance and enhanced understanding of infectious diseases.
- Published
- 2012
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