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1. Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression

Author

Juan A. Gerez, Natalia C. Prymaczok, Harindranath Kadavath, Dhiman Ghosh, Matthias Bütikofer, Yanick Fleischmann, Peter Güntert, and Roland Riek

Subjects
Biology (General), QH301-705.5
Abstract

An improved method for in-cell NMR is developed using a reporter to evaluate protein delivery and delivery parameters to different cell lines and applied to IDPs and folded proteins including structure determination of GB1 at 1.1 Å resolution.

Published
2022
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