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1. A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells

Author

Simon G. P. Funnell, Babak Afrough, John James Baczenas, Neil Berry, Kevin R. Bewley, Rebecca Bradford, Clint Florence, Yann Le Duff, Mark Lewis, Ryan V. Moriarty, Shelby L. O. Connor, Karen L. Osman, Steven Pullan, Sujatha Rashid, Kevin S. Richards, Kimberly J. Stemple, and Ivana Knezevic

Subjects
Immunologic diseases. Allergy, RC581-607, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.

Published
2021
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2. A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells

Author

Karen L. Osman, Ivana Knezevic, Sujatha Rashid, Kimberly J. Stemple, John J. Baczenas, Neil Berry, Steven T. Pullan, Babak Afrough, Mark G. Lewis, Clint Florence, Simon G. P. Funnell, Rebecca Bradford, Yann Le Duff, Kevin S. Richards, Kevin R. Bewley, Ryan V. Moriarty, and Shelby L. O. Connor

Subjects
0301 basic medicine, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), viruses, Immunology, Biology, Cleavage (embryo), Virus, Article, 03 medical and health sciences, 0302 clinical medicine, In vivo, Virology, Pharmacology (medical), 030212 general & internal medicine, skin and connective tissue diseases, Furin, RC254-282, Pharmacology, Vaccines, SARS-CoV-2, In vitro toxicology, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC581-607, 030104 developmental biology, Infectious Diseases, Cell culture, Vero cell, biology.protein, Immunologic diseases. Allergy
Abstract

An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.

Published
2021

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