1. Restriction mapping in nanofluidic devices
- Author
-
Riehn, Robert, Lu, Manchun, Wang, Yan-Mei, Lim, Shuang Fang, Cox, Edward C., and Austin, Robert H.
- Subjects
Nucleases -- Research ,Molecules -- Research ,DNA -- Research ,Science and technology - Abstract
We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of [Mg.sup.2+] and EDTA. We have successfully used the restriction enzymes Sinai, Sacl, and Pacl, and have been able to measure the positions of restriction sites with a precision of [approximately equal to] 1.5 kbp in 1 min using single DNA molecules.
- Published
- 2005