1. Blockage of [A.sub.2A] and [A.sub.3] adenosine receptors decreases the desensitization of human [GABA.sub.A] receptors microtransplanted to Xenopus oocytes
- Author
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Roseti, Cristina, Palma, Eleonora, Martinello, Katiuscia, Fucile, Sergio, Morace, Roberta, Esposito, Vincenzo, Cantore, Gianpaolo, Arcella, Antonietta, Giangaspero, Felice, Aronica, Eleonora, Mascia, Addolorata, Gennaro, Giancarlo Di, Quarato, Pier Paolo, Manfredi, Mario, Cristalli, Gloria, Lambertucci, Catia, Marucci, Gabriella, Volpini, Rosaria, Limatola, Cristina, and Eusebi, Fabrizio
- Subjects
GABA -- Research ,GABA -- Physiological aspects ,GABA -- Genetic aspects ,Temporal lobe epilepsy -- Risk factors ,Temporal lobe epilepsy -- Genetic aspects ,Temporal lobe epilepsy -- Care and treatment ,Temporal lobe epilepsy -- Research ,Therapeutics, Experimental ,Science and technology - Abstract
We previously found that the endogenous anticonvulsant adenosine, acting through [A.sub.2A] and [A.sub.3] adenosine receptors (ARs), alters the stability of currents ([I.sub.GABA]) generated by GABAA receptors expressed in the epileptic human mesial temporal lobe (MTLE). Here we examined whether ARs alter the stability (desensitization) of [I.sub.GABA] expressed in focal cortical dysplasia (FCD) and in periglioma epileptic tissues. The experiments were performed with tissues from 23 patients, using voltage-clamp recordings in Xenopus oocytes microinjected with membranes isolated from human MTLE and FCD tissues or using patch-clamp recordings of pyramidal neurons in epileptic tissue slices. On repetitive activation, the epileptic GABAA receptors revealed instability, manifested by a large [I.sub.GABA] rundown, which in most of the oocytes ([approximately equal to] 70%) was obviously impaired by the new [A.sub.2A] antagonists ANR82, ANR94, and ANR152. In most MTLE tissue-microtransplanted oocytes, a new [A.sub.3] receptor antagonist (ANR235) significantly improved [I.sub.GABA] stability. Moreover, patch-clamped pyramidal neurons from human neocortical slices of periglioma epileptic tissues exhibited altered [I.sub.GABA] rundown on ANR94 treatment. Our findings indicate that antagonizing [A.sub.2A] and [A.sub.3] receptors increases the [I.sub.GABA] stability in different epileptic tissues and suggest that adenosine derivatives may offer therapeutic opportunities in various forms of human epilepsy. epilepsy | focal cortical dysplasia
- Published
- 2009