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Your search keyword '"Ammerlaan, Carola"' showing total 3 results
Start Over Author "Ammerlaan, Carola" Publisher national academy of sciences
3 results on '"Ammerlaan, Carola"'

1. Tubuloid culture enables long-term expansion of functional human kidney tubule epithelium from iPSC-derived organoids

Author

Afd Pharmacology, Pharmacology, Yengej, Fjodor A. Yousef, Jansen, Jitske, Ammerlaan, Carola M. E., Dilmen, Emre, Casellas, Carla Pou, Masereeuw, Rosalinde, Hoenderop, Joost G., Smeets, Bart, Rookmaaker, Maarten B., Verhaar, Marianne C., Clevers, Hans, Afd Pharmacology, Pharmacology, Yengej, Fjodor A. Yousef, Jansen, Jitske, Ammerlaan, Carola M. E., Dilmen, Emre, Casellas, Carla Pou, Masereeuw, Rosalinde, Hoenderop, Joost G., Smeets, Bart, Rookmaaker, Maarten B., Verhaar, Marianne C., and Clevers, Hans

Published
2023

2. Tubuloid culture enables long-term expansion of functional human kidney tubule epithelium from iPSC-derived organoids

Author

Nefro Vasculaire Geneeskunde, MS Nefrologie, Regenerative Medicine and Stem Cells, Circulatory Health, CMM, Cancer, Hubrecht Institute with UMC, Yousef Yengej, Fjodor A., Jansen, Jitske, Ammerlaan, Carola M.E., Dilmen, Emre, Casellas, Carla Pou, Masereeuw, Rosalinde, Hoenderop, Joost G., Smeets, Bart, Rookmaaker, Maarten B., Verhaar, Marianne C., Clevers, Hans, Nefro Vasculaire Geneeskunde, MS Nefrologie, Regenerative Medicine and Stem Cells, Circulatory Health, CMM, Cancer, Hubrecht Institute with UMC, Yousef Yengej, Fjodor A., Jansen, Jitske, Ammerlaan, Carola M.E., Dilmen, Emre, Casellas, Carla Pou, Masereeuw, Rosalinde, Hoenderop, Joost G., Smeets, Bart, Rookmaaker, Maarten B., Verhaar, Marianne C., and Clevers, Hans

Published
2023

3. Tubuloid culture enables long-term expansion of functional human kidney tubule epithelium from iPSC-derived organoids.

Author

Yengej, Fjodor A. Yousef, Jansen, Jitske, Ammerlaan, Carola M. E., Dilmen, Emre, Casellas, Carla Pou, Masereeuw, Rosalinde, Hoenderop, Joost G., Smeets, Bart, Rookmaaker, Maarten B., Verhaar, Marianne C., and Clevers, Hans

Subjects
KIDNEY tubules, INDUCED pluripotent stem cells, KIDNEY development, ORGANOIDS, EPITHELIUM
Abstract

Kidney organoids generated from induced pluripotent stem cells (iPSC) have proven valuable for studies of kidney development, disease, and therapeutic screening. However, specific applications have been hampered by limited expansion capacity, immaturity, off-target cells, and inability to access the apical side. Here, we apply recently developed tubuloid protocols to purify and propagate kidney epithelium from d7+18 (post nephrogenesis) iPSC-derived organoids. The resulting 'iPSC organoid-derived (iPSCod)' tubuloids can be exponentially expanded for at least 2.5 mo, while retaining expression of important tubular transporters and segment-specific markers. This approach allows for selective propagation of the mature tubular epithelium, as immature cells, stroma, and undesirable off-target cells rapidly disappeared. iPSCod tubuloids provide easy apical access, which enabled functional evaluation and demonstration of essential secretion and electrolyte reabsorption processes. In conclusion, iPSCod tubuloids provide a different, complementary human kidney model that unlocks opportunities for functional characterization, disease modeling, and regenerative nephrology. [ABSTRACT FROM AUTHOR]

Published
2023
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