1. Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula
- Author
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Qi Feng, Jiaojiao Ji, Xue-Jun Zhang, Jianping Gao, Hai-Feng Sun, Xiao-xiao Li, and Jiankuan Li
- Subjects
0106 biological sciences ,Metabolite ,metabolite ,Pharmaceutical Science ,Polysaccharide ,01 natural sciences ,MeJA ,Analytical Chemistry ,Codonopsis pilosula ,lcsh:QD241-441 ,03 medical and health sciences ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Biosynthesis ,Drug Discovery ,Physical and Theoretical Chemistry ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Methyl jasmonate ,biology ,Organic Chemistry ,Glycoside ,biology.organism_classification ,Terpenoid ,chemistry ,Biochemistry ,Chemistry (miscellaneous) ,gene expression ,Molecular Medicine ,Subculture (biology) ,1H-NMR ,010606 plant biology & botany - Abstract
Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µ, mol·, L&minus, 1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µ, 1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in &beta, d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.
- Published
- 2019
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