1. Protective Effect of the Ethyl Acetate Fraction of Sargassum muticum Against Ultraviolet B–Irradiated Damage in Human Keratinocytes
- Author
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Dong Sam Kim, Mei Jing Piao, Weon Jong Yoon, Hee Kyoung Kang, Jin Won Hyun, Young Sang Koh, Eun Sook Yoo, and Nam Ho Lee
- Subjects
Keratinocytes ,Antioxidant ,medicine.medical_treatment ,Sargassum muticum ,Apoptosis ,Acetates ,medicine.disease_cause ,Antioxidants ,Lipid peroxidation ,lcsh:Chemistry ,chemistry.chemical_compound ,skin and connective tissue diseases ,lcsh:QH301-705.5 ,Spectroscopy ,chemistry.chemical_classification ,reactive oxygen species ,biology ,integumentary system ,General Medicine ,Catalase ,Computer Science Applications ,Biochemistry ,Cell Survival ,Ultraviolet Rays ,HaCaT cells ,Radiation-Protective Agents ,DNA Fragmentation ,Catalysis ,Article ,Cell Line ,Inorganic Chemistry ,Superoxide dismutase ,Picrates ,medicine ,Humans ,Physical and Theoretical Chemistry ,Molecular Biology ,Cell damage ,Reactive oxygen species ,Plant Extracts ,Superoxide Dismutase ,Organic Chemistry ,Biphenyl Compounds ,Sargassum ,Hydrogen Peroxide ,medicine.disease ,ultraviolet B ,apoptosis ,HaCaT ,Oxidative Stress ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,biology.protein ,Lipid Peroxidation ,Oxidative stress - Abstract
The aim of this study was to investigate the cytoprotective properties of the ethyl acetate fraction of Sargassum muticum (SME) against ultraviolet B (UVB)-induced cell damage in human keratinocytes (HaCaT cells). SME exhibited scavenging activity toward the 1,1-diphenyl-2-picrylhydrazyl radicals and hydrogen peroxide (H(2)O(2)) and UVB-induced intracellular reactive oxygen species (ROS). SME also scavenged the hydroxyl radicals generated by the Fenton reaction (FeSO(4) + H(2)O(2)), which was detected using electron spin resonance spectrometry. In addition, SME decreased the level of lipid peroxidation that was increased by UVB radiation, and restored the level of protein expression and the activities of antioxidant enzymes that were decreased by UVB radiation. Furthermore, SME reduced UVB-induced apoptosis as shown by decreased DNA fragmentation and numbers of apoptotic bodies. These results suggest that SME protects human keratinocytes against UVB-induced oxidative stress by enhancing antioxidant activity in cells, thereby inhibiting apoptosis.
- Published
- 2011