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În prezentul studiu a fost evaluat efectul acetaţilor de Cu(II) şi Zn(II) cu şi fără adaos de glucoză şi al iluminării asupra productivităţii şi producerii polizaharidelor intracelulare la cianobacteria Spirulina platensis. A fost analizată componenţa fracţiilor polizaharidice obţinute la separare prin cromatografia pe coloana de Toyo-Pearl şi stabilită natura lor proteoglicanică. A fost propusă schema de realizare a unui procedeu de extragere şi purificare a complexului de polizaharide din biomasă. STUDY OF POLYSACHARIDES PRODUCED BY CYANOBACTERIA SPIRULINA PLATENSIS IN MIXTROPHIC CULTIVATIONIn the present work, the authors studied the effect of Cu (II) and Zn (II) acetates with and without addition of glucose and the influence of two illumination regimes on the productivity and production of intracellular polysaccharides in cyanobacterium Spirulina platensis. The composition of the polysaccharide fractions obtained by chromatographic separation on a Toyo-Pearl column was analyzed and their proteoglycanic nature was determined. A scheme for the extraction and purification procedure of the polysaccharide complex from biomass has been proposed.

Au fost obţinute preparate de Se-EPZ şi EPZ din cianobacteria Spirulina platensis cultivată pe mediu nutritiv cu şi fără adaos de selenit. Extractele parţial purificate (după înlăturarea fracţiei proteice) au fost supuse separării prin metoda HPLC pe coloana de Toyo-Pearl HW40F. Au fost scanate spectrele UV ale preparatelor de exopolizaharide şi elaborată schema tehnologică de realizare a unui procedeu de obţinere a Se-EPZ produse de Spirulina platensis. PROCESS FOR OBTAINING OF Se-EXOPOLISAHARIDES PRODUCED BY CYANOBACTERIUM SPIRULINA PLATENSISSelenium-containing (Se-EPS) and EPS from Spirulina platensis cyanobacterium grown on the nutritive medium with and without selenite supplementation were obtained. The partially purified extracts (after removal of the proteins fraction) were subjected to HPLC separation on the Toyo-Pearl HW40F column. The UV spectra of the exopoly­sac­charide preparations have been scanned and the technological scheme for obtaining of Se-EPZ produced by Spirulina platensis has been developed.

A fost efectuată evaluarea comparativă a hidrolizatelor papainice şi triptice ale ficocianinei şi Se-ficocianinei în urma separării prin metoda HPLC cu faze inverse pe coloana de ResourceTMRPC. A fost analizat profilul cromatografic al hidrolizatelor papainice şi triptice ale ficocianinei la separare prin gel-filtrare pe coloana de sefadex G-50. Spectrele UV-VIS ale fracţiilor peptidice obţinute la separarea prin gel-filtrare a hidrolizatelor ficocianinei au scos în evidenţă pre­zenţa peptidelor legate cu cromoforul.EVALUATION OF PEPTIDES SPECTRUM AT FRACTIONATION OF ENZYMATIC HYDROLYSATES OF PHYCOCYANIN AND Se-PHYCOCYANIN BY CHROMATOGRAPHIC METHODSA comparative evaluation of papain and trypsin hydrolysates of phycocyanin and Se-phycocyanin after separation by HPLC with reverse phase on the ResourceTMRPC column was performed.The chromatographic profile of peptides from the trypsin and papain hydrolysates of phycocyanin at separation by gel filtration on Sephadex G-50 column was analyzed. UV-VIS spectra of peptides fractions obtained by gel filtration separation of phycocyanin hydrolysates revealed the presence of chromopeptides.

Dezvoltarea şi aplicarea tehnologiilor de inginerie enzimatică, folosind enzimele pentru hidroliza ficocianinei şi producerea peptidelor bioactive, deschide noi perspective de utilizare a lor în calitate de agenţi anticancer, antimicro-bieni, antioxidativi, antihipertensivi etc. Lărgirea spectrului de peptide produse în baza enzimolizei ficocianinei poate fi efectuată prin utilizarea în acest scop a enzimelor proteolitice cu specificitate diferită. În lucrare sunt analizate spectrele UV ale hidrolizatelor triptice sumare ale ficocianinei şi descrise unele metode de separare şi analiză a fracţiilor peptidice din hidrolizat.ALGAL BIOACTIVE PEPTIDES AND THEIR PERSPECTIVE USE AS THERAPEUTIC AGENTSDevelopment and application of enzyme engineering technologies using enzymes for the hydrolysis of Phycocyanin and production of bioactive peptides opens up new prospects for their use as anticancer, antimicrobial, antioxidant, hypotensive agents. Diversification of the range of produced peptides can be performed by the enzymolysis and using of the proteolytic enzymes with diverse specificity for this purpose. The paper presents the UV spectra of summary tryptic phycocyanin hydrolysates and some methods of separation and analyzing of peptide fractions.