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The quantification of endogenously present compounds in biological samples demands appropriately validated methods, in particular because increasing research efforts are aimed at studying the impact of such compounds on human health and disease. International regulatory agencies, such as the Food and Drug Administration (FDA) and the European Medicine Agency (EMA), have published a vast number of guidelines concerning bioanalytical method validation over recent decades. Compared with the quantification of exogenous compounds, these guidelines have remained rather vague and unclear when it comes to the quantification of endogenous compounds. Nonetheless, the continuous expansion of studies devoted to the search for endogenous disease markers in the human metabolome has incited the regulatory bodies to include endogenous compounds in a draft of an updated International Conference on Harmonization (ICH) guideline on bioanalytical method validation. In light of these recent developments, the main objective of this review article is to provide a clear summary of the different methods that can be used to quantify endogenous small molecules. Because of the increased use of mass spectrometry (MS) in the field of bioanalysis, a special focus will be placed on quantification by liquid chromatography (LC)--MS. Practical recommendations to face this bioanalytical challenge, in particular in terms of method validation, will also be provided. [ABSTRACT FROM AUTHOR]

The 44th International Symposium of High Performance Liquid Phase Separations and Related Techniques (HPLC 2016), chaired by Professor Robert Kennedy, was held 19-24 June in San Francisco, California, USA, at the Marriott San Francisco Marquis. This instalment of "Column Watch" covers some of the highlights observed at the symposium, including stationary-phase developments, particle technology, and areas of growing application of high performance liquid chromatography (HPLC). In addition, trends and perspectives on future developments in HPLC noted from the conference are presented. [ABSTRACT FROM AUTHOR]

The 48th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2019), chaired by Alberto Cavazzini and Massimo Morbidelli, was held from 16 to 20 June in Milan, Italy. This instalment of “Column Watch” covers many of the highlights observed at the symposium. In addition, trends and perspectives on future developments in HPLC, and related techniques gleaned from the conference, are presented. [ABSTRACT FROM AUTHOR]

The article focuses on the 45th International Symposium on High Performance Liquid Phase (HPLC) Separations and Related Techniques 2017, held during June 18-22, 2017 at the Prague Conference Center in Czech Republic. It highlights lecture, entitled Searching for Life on Ocean Worlds with Liquid Chromatography presented by Peter Willis.

The 44th International Symposium of High Performance Liquid Phase Separations and Related Techniques (HPLC 2016), chaired by Professor Robert Kennedy, was held June 19-24 in San Francisco, California, at the Marriott San Francisco Marquis. This installment of "Column Watch" covers some of the highlights observed at the symposium, including stationary-phase developments, particle technology, and areas of growing application of high performance liquid chromatography (HPLC). In addition, trends and perspectives on future developments in HPLC noted from the conference are presented. [ABSTRACT FROM AUTHOR]