1. Properties of Proteus mirabilis phage 13vir
- Author
-
J. A. Smit and J. N. Coetzee
- Subjects
biology ,Strain (chemistry) ,Serial dilution ,viruses ,Mitomycin C ,Operator gene ,biology.organism_classification ,Proteus mirabilis ,Virology ,Microbiology ,Ultraviolet light ,Bacteriophages ,Adsorption ,Prophage ,Bacteria - Abstract
Phage 13vir is a sheath-tailed phage (Prozesky, de Klerk & Coetzee, 1965, Pl. 1, fig. 5) which arises from single clones of Proteus mirabilis strain 13 (Coetzee & Sacks, 1960). It forms large clear plaques on strain 13. The phage may carry a constitutive mutation in an operator gene which controls development of the prophage in strain 13. It is not inducible with ultraviolet light or mitomycin C but is often present in overnight broth cultures of strain 13. The phage is particularly troublesome in that strain 13 is the host of transducing phage 34 (Coetzee & Sacks, 1960) and lysates of the latter are usually contaminated with phage 13vir. When high titre φ 13vir. 13 (prepared by infecting a broth culture of strain 13) is spotted on a lawn of a locally isolated P. mirabilis strain 5006 the bacteria are killed but serial dilutions of the phage only sporadically form plaques.
- Published
- 1970
- Full Text
- View/download PDF