1. In vitro antifungal susceptibilities of Candida species to liposomal amphotericin B, determined using CLSI broth microdilution, and amphotericin B deoxycholate, measured using the Etest
- Author
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Giuseppina Caggiano, Giusy Diella, Maria Teresa Montagna, Serafina Rutigliano, G Lovero, and Osvalda De Giglio
- Subjects
0301 basic medicine ,Microbiology (medical) ,Antifungal Agents ,030106 microbiology ,Candida glabrata ,Microbial Sensitivity Tests ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Pathogenicity and Virulence/Host Response ,Disk Diffusion Antimicrobial Tests ,Drug Resistance, Fungal ,Amphotericin B ,Amphotericin B deoxycholate ,Candida krusei ,parasitic diseases ,Candida albicans ,medicine ,Humans ,030212 general & internal medicine ,liposomal amphotericin B ,Etest ,Candida ,biology ,Candida lusitaniae ,E-test ,Broth microdilution ,General Medicine ,biology.organism_classification ,bacterial infections and mycoses ,Drug Combinations ,amphotericin B deoxycholate ,CLSI broth microdilution ,medicine.drug ,Research Article ,Deoxycholic Acid - Abstract
The antifungal susceptibilities of 598 isolates of Candida spp. (bloodstream and other sterile sites) to liposomal amphotericin B (L-AmB) versus amphotericin B (AmB) were determined. MICs were calculated using the Clinical and Laboratory Standards Institute broth microdilution (M27-A3) method for L-AmB and the Etest method for AmB. The MIC50/MIC90 (µg ml−1) values for L-AmB broth microdilution and AmB Etest were 0.25/1 and 0.19/0.5, respectively. The overall essential agreement (±2 dilutions) was 91.5 %, ranging from 37.5 % (Candida lusitaniae) to 100 % (Candida glabrata and Candida krusei). Categorical agreement between the two methods was categorized based on a previously published breakpoint (susceptible/resistant MIC cut-off of 1 µg ml−1). The overall categorical agreement at the 48 h reading was 97.3 %, ranging from 72.7 % (C. krusei) to 100 % (Candida albicans). Major and very major discrepancies occurred in 2.3 and 0.3 %, respectively. Spearman’s ρ was 0.48 (P
- Published
- 2017