1. A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model
- Author
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M. Perouse de Montclos, Jean-Pierre Flandrois, Gregory Devulder, Bioinformatique, phylogénie et génomique évolutive (BPGE), Département PEGASE [LBBE] (PEGASE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), and Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
DNA, Bacterial ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,Chaperonins ,Sequence analysis ,Computational biology ,Biology ,Polymerase Chain Reaction ,Microbiology ,DNA sequencing ,Mycobacterium ,Bacterial Proteins ,Phylogenetics ,RNA, Ribosomal, 16S ,Animals ,Humans ,Gene ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Genetics ,Phylogenetic tree ,Superoxide Dismutase ,Genes, rRNA ,Chaperonin 60 ,DNA-Directed RNA Polymerases ,Sequence Analysis, DNA ,General Medicine ,Ribosomal RNA ,rpoB ,biology.organism_classification ,Bacterial Typing Techniques - Abstract
Advances in DNA sequencing and the increasing number of sequences available in databases have greatly enhanced the bacterial identification process. Several species within the genusMycobacteriumcause serious human and animal diseases. In order to assess their relative positions in the evolutionary process, four gene fragments, from the 16S rRNA (564 bp),hsp65(420 bp),rpoB(396 bp) andsod(408 bp) genes, were sequenced from 97 strains, including all available type strains of the genusMycobacterium. The results demonstrate that, in this case, the concatenation of different genes allows significant increases in the power of discrimination and the robustness of the phylogenetic tree. The sequential and/or combined use of sequences of several genes makes it possible to refine the phylogenetic approach and provides a molecular basis for accurate species identification.
- Published
- 2005
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