1. New hepatitis C virus genotype 1 subtype naturally harbouring resistance-associated mutations to NS5A inhibitors
- Author
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Mar Riveiro-Barciela, Josep Quer, Laura Ordeig, María Eugenia Soria, Josep Gregori, Damir Garcia-Cehic, Leonardo Nieto-Aponte, Meritxell Llorens, Juan Ignacio Esteban, Qian Chen, Celia Perales, Rafael Esteban, Francisco Rodriguez-Frias, and Maria Buti
- Subjects
0301 basic medicine ,Pyrrolidines ,Genotype ,Proline ,Hepatitis C virus ,Gene Expression ,Hepacivirus ,Microbial Sensitivity Tests ,Viral Nonstructural Proteins ,Biology ,medicine.disease_cause ,Antiviral Agents ,Genome ,Deep sequencing ,Virus ,03 medical and health sciences ,0302 clinical medicine ,2-Naphthylamine ,Virology ,Drug Resistance, Viral ,medicine ,Humans ,Anilides ,Uracil ,NS5A ,Genotyping ,Phylogeny ,Fluorenes ,Sulfonamides ,Imidazoles ,Valine ,Sequence Analysis, DNA ,Middle Aged ,Hepatitis C ,Subtyping ,030104 developmental biology ,Equatorial Guinea ,Mutation ,Benzimidazoles ,Female ,030211 gastroenterology & hepatology ,Carbamates - Abstract
Hepatitis C virus (HCV) is a highly divergent virus currently classified into seven major genotypes and 86 subtypes (ICTV, June 2017), which can have differing responses to therapy. Accurate genotyping/subtyping using high-resolution HCV subtyping enables confident subtype identification, identifies mixed infections and allows detection of new subtypes. During routine genotyping/subtyping, one sample from an Equatorial Guinea patient could not be classified into any of the subtypes. The complete genomic sequence was compared to reference sequences by phylogenetic and sliding window analysis. Resistance-associated substitutions (RASs) were assessed by deep sequencing. The unclassified HCV genome did not belong to any of the existing genotype 1 (G1) subtypes. Sliding window analysis along the complete genome ruled out recombination phenomena suggesting that it belongs to a new HCV G1 subtype. Two NS5A RASs (L31V+Y93H) were found to be naturally combined in the genome which could limit treatment possibilities in patients infected with this subtype.
- Published
- 2018
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