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A Gram-negative, non-flagellated, rod-shaped bacterium, designated strain P2K6(T), was isolated from the kidneys of a pufferfish (Arothron hispidus) caught off the coast of Kaneohe Bay, O'ahu, Hawai'i. The strain formed yellowish colonies when grown on marine agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain P2K6(T) was related most closely to members of the genus Chryseobacterium. Levels of 16S rRNA gene sequence similarity between strain P2K6(T) and the type strains of recognized species of the genus Chryseobacterium were 94-96.6%, suggesting that the strain represents a novel species within this genus. The DNA G+C content of strain P2K6(T) was 36.5 mol%, the dominant fatty acids were iso-C(15:0) (35.3%) and iso-C(17:0) 3-OH (14.9%), and the most abundant quinone was menaquinone MK6. On the basis of the data from this polyphasic study, it is suggested that strain P2K6(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium arothri sp. nov. is proposed. The type strain is P2K6(T) (=CIP 109575(T)=DSM 19326(T)).

A novel species, strain P2S11T, was isolated from the mucus of a puffer fish caught off the coast of Kaneohe Bay, O'ahu, Hawai'i. A phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was most closely related to Ferrimonas marina DSM 16917T and Ferrimonas balearica DSM 9799T with 93.5% and 82.9% sequence similarities, respectively, which established the novel strain as belonging to the genus Ferrimonas. The strain formed off-white coloured colonies on marine agar and cells were Gram-negative, non-motile rods. H2S was produced when strain P2S11T was grown on TSI medium with added salt. Strain P2S11T had a DNA G+C content of 54.9 mol% and the dominant fatty acids were C16:1omega9c, C16:0 and C17:1omega8c. On the basis of this polyphasic study, strain P2S11T (=ATCC BAA-1480T=DSM 18821T) represents a novel species of the genus Ferrimonas, for which the name Ferrimonas senticii sp. nov. is proposed.

An aerobic, mesophilic bacterium, strain JA40T, was isolated from soil contaminated with polycyclic aromatic hydrocarbons and polychlorinated biphenyls collected from Johnston Atoll in the North Pacific Ocean. The strain formed yellow-pigmented colonies on heterotrophic media. The cells were Gram-negative, non-motile, non-sporulating rods. The strain reduced nitrite to nitrous oxide, the DNA G+C content was 64 mol% and the dominant fatty acids were 15 : 0 iso, 17 : 1 iso cis7 and 11 : 0 iso 3-OH. DNA sequencing of 1457 nt of the 16S rRNA gene established that JA40T belongs in the genus Pseudoxanthomonas within the Xanthomonadaceae branch of the Gammaproteobacteria. Strain JA40T can be differentiated from other mesophilic species in the genus on the basis of its physiological and biochemical characteristics and distinctive fatty acid profile. Thus strain JA40T (=ATCC BAA-1031T=CIP 108476T) is the type strain of a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas kalamensis sp. nov. is proposed.