Background: Mineral trioxide aggregate (MTA) becomes a hard mass after setting and making it difficult to remove and can cause significant problems in the retreatment process. The aim of this study was to evaluate the effect of different concentrations of hydrochloric acid (HCl) on MTA dissolution and its effect on dentin., Materials and Methods: In this in vitro study, 45 single-root premolars were selected. Artificially open apex was created in all samples with similar process. The samples were randomly divided into 4 experimental groups of 10, and a control group of 5. Four-millimeter thick apical plugs of Root MTA were placed in all samples in an orthograde manner. HCl was used at concentrations of 3.75%, 7.5%, 15%, and 22.5% (w/v) for the experimental groups and normal saline for the control group. Each sample was exposed to the desired solution for 15 min. Then, MTA retrieval and reaching the working length were attempted with k-file # 30. The times of each sample were recorded. Furthermore, after longitudinal incision of the roots with a disc, the dentin surfaces of canals were examined with a Dino-Lite microscope (×50). Results were analyzed by Shapiro-Wilk test and one-way analysis of variance tests. The level of significance P value was set at 0.05., Results: The lowest average time of reaching working length was observed with group 22.5% that was significantly lower than 15% and 7.5% concentrations ( P = 0.005 and P = 0.011). Furthermore, by examining with ×50 of Dino-Lite microscope, no difference was observed on the canal walls., Conclusion: The optimum concentration of HCl was 7.5%. Furthermore, different concentrations of HCl had no significantly different effect on the dentinal canal wall using Dino-Lite microscope with ×50., Competing Interests: The authors of this manuscript declare that they have no conflicts of interest, real or perceived, financial or nonfinancial in this article., (Copyright: © 2023 Dental Research Journal.)