1. Effective Isolation for Lung Carcinoma Cells Based on Immunomagnetic Separation in a Microfluidic Channel.
- Author
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Vu-Dinh H, Feng H, and Jen CP
- Subjects
- A549 Cells, Cell Separation, Early Detection of Cancer, High-Throughput Screening Assays, Humans, Microscopy, Fluorescence, Aptamers, Nucleotide chemistry, Immunomagnetic Separation methods, Lung Neoplasms diagnosis, Microfluidic Analytical Techniques methods
- Abstract
In this paper, we developed an isolation system for A549 human lung carcinoma cells as an effective factor for the early diagnosis of lung cancer. A microfluidic immunomagnetic method was used, in which the combination of immunomagnetic separation and a microfluidic system allowed for increased isolation efficiency with uncomplicated manipulation. In the microfluidic immunomagnetic strategy, A549 cells were combined with aptamer-conjugated carboxylated magnetic beads and then collected in a specified region by applying a magnetic field. The results were recorded using a fluorescence microscope, and the captured targets were then quantified. The isolation efficiency of A549 cells is up to 77.8%. This paper developed a simple working procedure, which is less time consuming, high-throughput, and trustworthy for the isolation of A549 cells. This procedure can be a useful reference method for the development of an effective diagnosis and treatment method for lung cancer in the future.
- Published
- 2021
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