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The Magnisi peninsula is a small portion of land located near the largest Italian petrol-chemical pole of Augusta–Priolo–Melilli (40 km2), which, since the 1950s, devastated the local environment and landscape and unloaded directly into the sea an impressive quantity of pollutants. Unlike the terrestrial part of the area, where a Natural Oriented Reserve (NOR) called “Saline di Priolo” was established in the 2000s, no concrete legislative action has been implemented or proposed for the marine environment. At the same time, the fauna of the marine environment has not been studied in the same way as that of the terrestrial environment. As concerns the molluscan fauna, most of the information dates back to the 1800s. These studies were exclusively focused on the shells of some mollusks in the area. Instead, no study related to this area has ever been carried out on the group of sea slugs. This study conducted between 2022 and 2023, through snorkeling activities, allowed to provide the first faunistic list of the sea slugs of this area, together with information on the biology and ecology of these gastropods, highlighting the potential biodiversity present in this small stretch of coastline affected by high industrial pollution.

Thousands of theatres were built during the Hellenistic period in Greece and overseas colonies. The main elements of the Hellenistic theatre are the following: the orchestra, where music and songs were performed to accompany the acting performance, and the koilon, where the audience sat. Hellenistic theatres were built without any ceiling, with an open-air configuration. This paper reports the acoustic characteristics of the Greek (Hellenistic) theatre located in Morgantina (Sicily, Italy) based on the technical data gathered in different listening positions selected across the sitting area (koilon). The theatre of Morgantina was built in the third century BC and renovated a few decades ago. Nowadays, it is the center of important social and cultural activities. The outcomes of the beamforming technique employed for the survey have been discussed in comparison with traditional acoustic parameters, such as ISO 3382. The scope of this article is to assess the usability of this theatre intended to be used for different types of artistic performances.

The geographic position, isolation, and the long and dynamic history of colonization created a human context in Sicily that allows for a particular anthropological study; information about “migratory flow” and “population influx” could be investigated in the cranial morphology of a localized geographical region. The research goals are the identification of temporal trends in facial morphology in order to assess the adaptations and the microevolutionary trends and to verify if the cranial morphology of humans was modified by the various genetic contributions and more or less related to the intense and significant migratory flows. This work includes a diachronic morphometrics study of 3D models of 95 Sicilian skulls coming from 19 populations (from the Paleolithic to the Contemporary Age), providing an overview of human biodiversity and variability in Sicily. To achieve this, a geometric morphometrics analysis of the facial features of adult human skulls was performed. The approach used allows for the identification of the main micro-anatomical and micro-evolutionary features. Considering sample size/composition, it has been possible to discriminate between prehistorical and historical populations. The results highlight a series of morphological changes related to different migratory flows that have followed one another with different intensities and effectiveness starting from the Prehistory up to the Contemporary Age. The human peopling of Sicily is a subject of continuous debate; however, this study points to the coexistence of microevolutionary patterns and population dynamics, with the latter being one of the main causes of the morphological variations.

The WAS—Waterscape Archaeology in Sicily—project is dedicated to underwater cultural heritage mapping, knowledge and awareness of the cultural heritage, dissemination, and analysis of the submerged environment concerning the coast. The prototype investigation site presented here is Isola delle Femmine (PA, Italy). This paper highlights the archaeological discovery with a description of the main finds, across a multidisciplinary approach, carried out with low-cost technology, increasing sustainable diving, and underlining the relationship between submerged and open-air historical evidence. Our studies address the realization of new underwater archaeological itineraries connected to local history. This text provides a historical-archaeological introduction to understanding the context of the site and, with some geological notes, illustrates the phases of the research with a presentation of the main artifacts, with a focus on the methodology and the techniques of the surveys and the usefulness of underwater photogrammetry and 3D modeling with a particular focus on the tourism application in the diving centers. Our work has allowed for the creation of two new underwater archaeological itineraries of Sicily. The innovative aspects of our project are linked to a new holistic approach in the context of the scientific synergy between multiple disciplines.

In this paper, my objective is to delve into the history of women accused of practicing witchcraft in Sicily during the early modern period. This investigation draws upon documented evidence from the Spanish Inquisition spanning from 1516 to 1782, along with archival records and the ethnographic works of nineteenth-century scholars. The focal point of my research is the enigmatic figure known as donna de fora (the ladies from outside) in the Italian context. To illuminate this subject, I employ an analysis of seventeenth-century Inquisition trial records and oral traditions documented by anthropologist Giuseppe Pitrè in the late nineteenth century. The donne de fora represent a distinctive and intriguing group as this term appears exclusively within the Inquisitorial records of Sicily. They were perceived as supernatural entities, characterized as “part witches, part fairies”. According to beliefs of the time, these women’s spirits would depart from their bodies during sleep to convene with higher-ranking fairies. Notably, the trials and the Edict of the Diocese of Girgenti (Agrigento) in 1656 document that the most significant gatherings of these figures occurred during the night of Saint John, between the 23rd and 24th of June. Through an examination of trial records, we gain insights into how these women were perceived by their contemporaries, as well as an understanding of their societal roles and the ritual practices they engaged in. Moving forward to the late nineteenth century, ethnologist Giuseppe Pitrè conducted a comprehensive study of local rituals and popular folklore, and he collected various objects and documents related to supernatural beliefs, including those associated with the donne de fora in the regions around Palermo. My research is centered on archival records containing Pitrè’s notes, unpublished drafts, and correspondence with scholars in Italy and Europe discussing this phenomenon. Based on my findings, I aim to establish a connection between Pitrè’s material discoveries and contemporary beliefs regarding donne de fora and witchcraft in Sicily.

Background: Reinfections occur as a response to natural infections wanes and novel strains of SARS-CoV-2 emerge. The present research explored the correlation between sex, age, COVID-19 vaccination, prior infection hospitalization, and SARS-CoV-2 reinfection in Sicily, Italy. Materials and Methods: A population-based retrospective cohort study was articulated using the vaccination flux from a regional registry and the Sicilian COVID-19 monitoring system of the Italian Institute of Health. Only adult Sicilians were included in the study, and hazard ratios were calculated using Cox regression. Results: Partial vaccination provided some protection (adj-HR: 0.92), when compared to unvaccinated individuals; furthermore, reinfection risk was reduced by full vaccination (adj-HR: 0.43), and the booster dose (adj-HR: 0.41). Males had a lower risk than females of reinfection with SARS-CoV-2 (adj-HR: 0.75). Reinfection with SARS-CoV-2 was diminished by hospitalization during the first infection (adj-HR: 0.78). Reinfection risk was higher among those aged 30–39 and 40–49 compared to those aged 18–29, whereas those aged 60–69, 70–79, and 80+ were statistically protected. Reinfection was significantly more frequent during the wild-type–Alpha, Delta, Delta–Omicron, and Omicron dominance/codominance waves compared to the wild type. Conclusions: This study establishes a solid base for comprehending the reinfection phenomenon in Sicily by pinpointing the most urgent policy hurdles and identifying some of the major factors. COVID-19 vaccination, one of the most effective public health tools, protects against reinfection, mostly caused by the Omicron strain. Elderly and hospitalized people’s lower risk suggests stricter PPE use.

A new species of the subfamily Pselaphinae (Coleoptera: Staphylinidae) has been discovered on Mount Etna (Sicily, Italy) and is described herein as Bryaxis aetnensis sp. nov. The new species is closely associated with the Bryaxis difficilis group, a highly homogeneous group of species living in the regions of Sicily and Sardinia. Diagnostic features and distribution of Sicilian species of this group are treated and illustrated herein. Bryaxis aetnensis sp. nov. exhibits similarities to B. marinae but can be distinguished by the darker color, longer antennal scape and terminal palpomere, and in the aedeagus morphology. The distribution of B. aetnensis sp. nov. spans a wide altitudinal range, demonstrating a remarkable climatic tolerance across the slopes and diverse habitats of Mount Etna. This broad tolerance reflects the species’ probable high ecological plasticity, which may also contribute to the observed morphological variability among individuals from different sampling sites. The significance of this new discovery on Mount Etna highlights the need to intensify sampling efforts in the region. Strengthening protection for these unexplored environments is crucial, and it also aids in unraveling biogeographic questions about the fauna inhabiting the area. As a relatively young volcanic environment, species colonization has occurred recently, making it an intriguing subject of investigation.

Human respiratory syncytial virus (hRSV) is an important pathogen of acute respiratory tract infection of global significance. In this study, we investigated the molecular epidemiology and the genetic variability of hRSV over seven surveillance seasons between 2015 and 2023 in Sicily, Italy. hRSV subgroups co-circulated through every season, although hRSV-B mostly prevailed. After the considerable reduction in the circulation of hRSV due to the widespread implementation of non-pharmaceutical preventive measures during the COVID-19 pandemic, hRSV rapidly re-emerged at a high intensity in 2022–2023. The G gene was sequenced for genotyping and analysis of deduced amino acids. A total of 128 hRSV-A and 179 hRSV-B G gene sequences were obtained. The phylogenetic analysis revealed that the GA2.3.5a (ON1) and GB5.0.5a (BA9) genotypes were responsible for the hRSV epidemics in Sicily.; only one strain belonged to the genotype GB5.0.4a. No differences were observed in the circulating genotypes during pre- and post-pandemic years. Amino acid sequence alignment revealed the continuous evolution of the G gene, with a combination of amino acid changes specifically appearing in 2022–2023. The predicted N-glycosylation sites were relatively conserved in ON1 and BA9 genotype strains. Our findings augment the understanding and prediction of the seasonal evolution of hRSV at the local level and its implication in the monitoring of novel variants worth considering in better design of candidate vaccines.

Biological invasions are widely recognized as a major threat to native biodiversity, ecosystem functioning and services. Amongst the NIS recorded in the Mediterranean Sea, Chlorophyta species belonging to the genus Caulerpa (Caulerpa cylindracea, C. taxifolia and C. taxifolia var. distichophylla) have raised serious concerns due to their potential or ascertained impact on native benthic communities. During surveys carried out to monitor the occurrence and the coverage of Caulerpa species along the coasts of Favignana Island (Egadi Islands Marine Protected Area), we observed variations in C. cylindracea and C. taxifolia coverage and also some large invertebrates overgrown with thalli of both species.

Among the fourteen towns most damaged by the earthquake that struck western Sicily in 1968, Poggioreale was subjected to total relocation a few kilometres away, and to the transfer of its whole population. More than half a century later, the damaged settlement is still largely recognisable and has been experiencing a new season of interest for some years now, both from the local community and various kinds of outsiders who imagine economic spin-offs of an uncertain nature in its enhancement. The research we present questions the chance of survival for the settlement, forgotten for decades, in the context of interventions aimed at its reuse. Alternative strategies are discussed: on the one hand, that of bringing the ancient centre back to life, even in part, with a limited inclusion of collective functions and without claiming to inhabit it again; on the other hand, and in the authors’ opinion, more likely, that of reusing the ruins as an archaeological area preserving their memory in a different form. In either case, the kinds of operations to be carried out on the body of the ancient buildings for their physical preservation play a key role. These issues are addressed as a whole through the canonical procedure of correlating the phases of knowledge, interpretation, and definition of minimal and compatible intervention criteria.

This research explores the figurative culture that flourished in Sicily during the 12th and 13th centuries, focusing on the interplay between artifacts of different types, materials, techniques and uses. Paintings, sculptures and objects that share a common visual language are analyzed with the aim of highlighting recurring motifs, mutual influences and related sources. The main focus is on the decorative apparatus of the Sacramentary Ms. 52 (Madrid, Biblioteca Nacional de España), one of the most famous illuminated manuscripts from Sicily. The date, origin and patronage of this luxurious liturgical book have been the subject of intense scholarly debate. In order to shed light on these controversial issues, this study re-examines the various hypotheses considered by scholars, taking into account the historical events that affected Sicily from the end of the Norman to the beginning of the Swabian era. This analysis also shows how the decoration of the manuscript fits into the wider dynamics of cultural exchange that characterized Sicily and the Mediterranean during this transitional period.

This study aims to clarify the taxonomic doubts, which have varied over the centuries, on the only two endemic species of the genus Pimpinella growing in Italy: P. anisoides and P. gussonei. For this purpose, the main carpological characters of the two species were examined, analyzing the external morphological traits and their cross-sections. Fourteen morphological traits were identified, obtaining datasets for the two groups using 40 mericarps (20 per species). The obtained measurements were subjected to statistical analysis (MANOVA and PCA). Our results highlight that at least 10 of the 14 morphological traits analyzed support the distinction between P. anisoides and P. gussonei. In particular, the following carpological characters are very significant ways to distinguish between the two species: monocarp width and length (Mw, Ml), monocarp length from base to maximum width (Mm), stylopodium width and length (Sw, Sl), length/width ratio (l/w) and cross-section area (CSa). In particular, the fruit of P. anisoides is larger (Mw 1.61 ± 0.10 mm) than that of P. gussonei (Mw 1.27 ± 0.13 mm), the mericarps of the first species are longer (Ml 3.14 ± 0.32 vs. 2.26 ± 0.18 mm) and the cross-section area (CSa) of P. gussonei is larger (0.92 ± 0.19 mm) than that of P. anisoides (0.69 ± 0.12 mm). The results also highlight the importance of the morphological traits of the carpological structures for the specific discrimination of similar species. The findings of this study contribute to an evaluation of the taxonomic significance of this species within the genus Pimpinella, and also provide valuable information for the conservation of these two endemic species.

Brown trout populations living in the limit of the distribution of the species face challenging environmental conditions. In islands, this vulnerable situation is exacerbated by geographical isolation. Sicilian trout persist only in the south-eastern part of the island and, based on their morphological characteristics, they have been recognized as a distinct species named Salmo cettii. We present the most extensive genetic study on Sicilian trout, based on 213 individuals from nine different streams in four basins. Sequencing of the mitochondrial control region and genotyping of the LDH-C* locus and eight microsatellite markers were carried out to evaluate hatchery introgression from past stocking practices in natural populations and to estimate the gene diversity distribution in populations. Results showed that only trout from the Tellesimo River remained free of hatchery introgression. Gene diversity was low in this native population, but increased in the rest of the samples, probably because of the introduction of hatchery genes. Despite the high level of introgression, the distribution of gene diversity depicted a clear natural pattern of population structure related to the hydrographic hierarchy. Because they represent long evolutionary histories, Sicilian trout populations should be considered of high conservation priority and managed according to at least four different genetic units.

The Mandorla di Avola is recognized all over the world as one of the best almond varieties. It is cultivated in a small area inside the provinces of Siracusa and Ragusa (Sicily, southern Italy). It is used in traditional Sicilian cuisine for both salty and sweet foods and of course in artisan pastry, apart from being consumed as a fruit. Due to its extraordinary organoleptic and beneficial features, the Mandorla di Avola is frequently counterfeit with almond varieties of lower quality coming from other countries. While its nutraceutical features have been studied, the possibility of authenticating it with respect to other varieties has not been explored. In this work, we used microelements determined with ICP-OES and ICP-MS as chemical descriptors to distinguish samples of Mandorla di Avola almonds from almonds coming from California and Spain, which are usually employed as substitutes in pastry. Among the different elements determined, Mn and P were found to be the best descriptors for authentication.

Major threats to the human lifespan include cancer, infectious diseases, diabetes, mental degenerative conditions and also reduced agricultural productivity due to climate changes, together with new and more devastating plant diseases. From all of this, the need arises to find new biopesticides and new medicines. Plants and microorganisms are the most important sources for isolating new metabolites. Lampedusa Island host a rich contingent of endemic species and subspecies. Seven plant species spontaneously growing in Lampedusa, i.e., Atriplex halimus L. (Ap), Daucus lopadusanus Tineo (Dl), Echinops spinosus Fiori (Es) Glaucium flavum Crantz (Gf) Hypericum aegypticum L: (Ha), Periploca angustifolia Labill (Pa), and Prasium majus L. (Pm) were collected, assessed for their metabolite content, and evaluated for potential applications in agriculture and medicine. The HPLC-MS analysis of n-hexane (HE) and CH2Cl2 (MC) extracts and the residual aqueous phases (WR) showed the presence of several metabolites in both organic extracts. Crude HE and MC extracts from Dl and He significantly inhibited butyrylcholinesterase, as did WR from the extraction of Dl and Pa. HE and MC extracts showed a significant toxicity towards hepatocarcinoma Huh7, while Dl, Ha and Er HE extracts were the most potently cytotoxic to ileocecal colorectal adenocarcinoma HCT-8 cell lines. Most extracts showed antiviral activity. At the lowest concentration tested (1.56 μg/mL), Dl, Gf and Ap MC extracts inhibited betacoronavirus HCoV-OC43 infection by> 2 fold, while the n-hexane extract of Pm was the most potent. In addition, at 1.56 μg/mL, potent inhibition (>10 fold) of dengue virus was detected for Dl, Er, and Pm HE extracts, while Pa and Ap MC extracts dampened infections to undetectable levels. Regarding to phytotoxicity, MC extracts from Er, Ap and Pm were more effective in inhibiting tomato rootlet elongation; the same first two extracts also inhibited seed cress germination while its radicle elongation, due to high sensitivity, was affected by all the extracts. Es and Gf MC extracts also inhibited seed germination of Phelipanche ramosa. Thus, we have uncovered that many of these Lampedusa plants displayed promising biopesticide, antiviral, and biological properties.

Sicilian wines have shown a growing expansion in the international market, and over 60% of the production of them is focused on quality products. Grillo is a white grape variety, and it is among the best-known variety, with a cultivated area of 6300 ha and with the vocation of being particularly predisposed to aging for years or even decades. This paper aimed to perform a physiochemical (SSC and pH) and polyphenolic characterization of Grillo wines that were produced by a selected winery in the years 2011–2021 using an optimized RP-HPLC-DAD method. The polyphenols fraction was assessed by means a semiquantitative analysis on which, statistical processing was carried out. The HCA and PCA highlighted the presence of three clusters in the samples. Cluster 1 was composed of the samples from the years 2011–2014, cluster 2 composed of the samples from 2015–2017, and cluster 3 composed of the samples from 2019–2021. Using an HSD Tukey test, it was possible to point out that some compounds were makers of specific clusters and therefore, specific vintages. This preliminary study showed that polyphenols are suitable markers that can be used to identify Grillo vintages, and they should be also related to the storage conditions or different production processes.

Atmospheric CO2 concentrations can reach high levels inside natural caves, representing a hazardous condition for both humans frequenting the underground environment and its safeguard due to the corrosion of speleothems induced by the acidification of atmospheric moisture. These issues are particularly critical for the eco-sustainable management of caves protected as nature reserves and undergoing touristic exploitation. In this paper we present the results of the C6 project, which was activated in 1999 for the monitoring of air quality inside three caves protected as nature reserves in Sicily (Italy). Near-real-time and spot measurements of air temperature and CO2 concentration have been carried out since the year 2000, giving the opportunity of evaluating the gas hazard for visitors and its potential impact on the protected underground environments, as well as the influence of meteorological and hydrological conditions in driving carbon dioxide accumulations. The analysis of data acquired in the hypogeal atmosphere, and their comparison with analogous epigeal measures, indicates that carbon dioxide accumulation is controlled by a complex interaction among cave topography, meteorological dynamics, gaseous exchanges between groundwaters and the atmosphere, and human fruition. This last factor, under particular conditions, can surprisingly diminishing underground CO2 concentrations.

Based on the critical review of the literature published in the last 22 years, an attempt was made to evaluate the current knowledge gap on the distribution and status of the native Testudines taxa occurring in Sicily (namely Caretta caretta, Emys trinacris, and Testudo hermanni hermanni), as well as the available knowledge of the only non-native species with putative viable populations occurring on the island, i.e., Trachemys scripta. Summarizing the current information, all of the Testudines species occurring in Sicily showed a fragmented and incompletely-known distribution, and only scarce data are available about their phenology. Moreover, despite their inclusion of international and national laws (Bern Convention, CITES, Habitat directive), all three native species are facing several threats (e.g., habitat alteration, the occurrence of invasive species, parasite spillover) leading to a reduction of their populations on the island. Future monitoring programs on the island should be enhanced, with an emphasis on those taxa in decline. Moreover, involve Citizen Science programs should also be implemented in order to increase the awareness of non-experts and facilitate the monitoring task.

Table grape (Vitis vinifera L.) is considered to be one of the most popular fruit crops in the world. Italy is the leading table-grape producer in the EU and is the main European exporting country. However, to stay competitive, new solutions and perspectives, including varietal renovation, are now needed in addition to the already well-established Italian table-grape production lines consisting of conventional open-field cultivation and greenhouse cultivation. One of these new perspectives is represented by the development of table-grape soil-less cultivation systems (TGSC) under greenhouse. In fact, TGSC systems are alleged to offer many advantages in terms of the advancement of berry maturity, extreme varietal flexibility, easier manipulation of the vegetative–reproductive cycle, higher yields of high quality extra-seasonal production, higher sustainability for reduced pesticides application, and higher use efficiency of water and fertilizers than conventional (soil-grown) cultivation. They can be also useful for overcoming soil- and rootstock-related problems. In this review, the opportunities offered by the recently developed table-grape soil-less cultivation systems are thoroughly examined and updated to the latest experimental and application findings of the sector’s research activity. A special emphasis is given to the evolution of the applied technical solutions, varietal choice, and environmental conditions for the aims of table-grape soil-less cultivation.

Anoxia (Protanoxia) orientalis is a beetle of the family Melolonthidae for which Italy represents the western limit of its distribution. The ecology of this species is little known from the quantitative point of view. The ecological correlates of A. orientalis presence in the whole European range and, more specifically, at its western border in Sicily, are analyzed in this paper to develop a potential distribution map for Sicily and to define the habitat selection of this species. There was a clear non-random habitat selection by A. orientalis at both the European and the Sicilian scales and a clear difference in the factors affecting the presence of this species in the larger spatial scale compared to Sicily. At the European scale, the bioclimatic factors were more important than landscape factors, whereas the same was not true at the Sicilian scale. In Sicily, the populations were statistically influenced by a combination of predictors that make their potential optimal distribution very narrow and mostly limited to a few coastal areas, suggesting a region-specific ecological diversification. Since A. orientalis is in strong decline in Italy due to the degradation of coastal environments, it is necessary to minimize the degradation of the dune and back dune environments in Sicily to achieve better management for the populations of this beetle species.

A syntaxonomical revision of the Isoëto-Nanojuncetea class for the Sicilian territory is provided. This syntaxon gathers the ephemeral herbaceous hygrophilous plant communities linked to periodically submerged soils, widely distributed in the European, circum-Mediterranean and Macaronesian territories. Within this class, two orders are recognized, lsoëtetalia, with a prevalently Mediterranean distribution, and Nanocyperetalia chiefly occurring in the central-European and Atlantic territories, with scattered and marginal occurrence in the Mediterranean area. The order Isoëtetalia in Sicily is represented by four alliances, i.e., Isoëtion, Preslion cervinae, Cicendio-Solenopsion laurentiae and Agrostion salmanticae, while within Nanocyperetalia three alliances can be recognized, namely Nanocyperion, Verbenion supinae and Lythrion tribracteati. Overall, 32 plant communities are recognized, 11 of which are described for the first time. Each higher rank syntaxa and related associations are examined from a nomenclatural, floristic, ecological and chorological point of view. In particular, the associations were processed using cluster analysis in order to highlight the correlations between them. Regarding the floristic aspects, a checklist of the species occurring in the phytosociological relevés is provided, as well as a new combination concerning Solenopsis gasparrinii, a critical species of the Sicilian flora, is proposed.

The phenomenon of insular dwarfism in proboscideans is particularly well represented on the Siculo-Maltese Palaeoarchipelago, an island group on which a species complex of palaeoloxodont elephants evolved during the Middle-Late Pleistocene. This likely included three species from Malta, four from Sicily (and possibly its palaeo-islands), and one from Favignana Island, ranging in size from the 1 m-tall Palaeoloxodon falconeri to continental-sized 4m-tall P. antiquus. However, our understanding of the causes for extreme differences in body size among insular samples in such a small geographic region is still limited. Here, I document the full range in size of elephants from the palaeoarchipelago, and discuss the reasons for size differences on the three islands in time and space in relation to predation, competition, resource limitation, accelerated life history, and duration of isolation. Differences in size between larger and smaller Sicilian elephants from Luparello Fissure, as well as possibly also in P. ex gr. P. mnaidriensis from Puntali Cave, San Teodoro Cave, and Favignana Island, may relate to the duration of isolation, or alternatively environmental stressors associated with the Last Glacial Maximum in the case of the Favignana elephant. Additionally, small but significant differences in size observable in Middle Pleistocene P. ex gr. P. falconeri from different localities on Sicily, as well as in Maltese P. ‘melitensis’ may also relate to duration of isolation, highlighting the need for better geochronological data in order to better distinguish macro-ecological causes driving body size change from more subtle effects relating to duration of isolation.

Bovine leptospirosis is an infectious zoonotic disease causing reproductive problems and economic losses in livestock. This work reports, for the first time in Sicily (South Italy), an outbreak of Leptospira interrogans serogroup Pomona that occurred in cattle farms within the Nebrodi Park and was mainly characterized by full-term abortion. Blood and urine samples were collected at different time points from animals of six different farms (Farms A–F) sharing the same grazing area. Research of antibodies against pathogenic Leptospira species in serum samples was carried out via Micro Agglutination Test (MAT). Urine samples were subjected to pathogen isolation and molecular analyses via TaqMan Real Time-PCR. Genotyping of Leptospira species was obtained by Multi-locus sequence typing. MAT detected antibodies against Leptospira interrogans serogroup Pomona in serum samples of all the farms. Pathogenic Leptospira spp. DNA and culture isolation was obtained from urine samples. Genotyping confirmed the excretion of L. interrogans serogroup Pomona. This study describes clinical manifestations, diagnostic implications and epidemiological characteristics of an outbreak in cattle due to L. interrogans Pomona in a protected multi-host area, where domestic and wild animals share the same habitat, suggesting a role of wild species in transmission and persistence of Pomona serogroup among cattle.

Leptospirosis is a re-emerging zoonosis of worldwide significance; a wide spectrum of wild and domestic animal species act as natural or accidental hosts. Swine can act as maintenance or accidental hosts of pathogenic Leptospira spp. This study aimed at investigation of Leptospira spp. prevalence and diversity in slaughtered pigs in southern Italy (Sicily). In total, 55 samples of kidneys and blood were collected. Microscopic agglutination test and real-time PCR were performed to detect pathogenic and intermediately pathogenic Leptospira. Partial rpoB gene sequencing and multi-locus sequence typing (MLST) were performed to characterize Leptospira species. The analysis showed a seropositivity rate of 16.4%, with Australis representing the most frequently identified serogroup (63.64%); Pomona and Sejroe were detected with a prevalence of 27.27% and 9.09%, respectively. Pathogenic Leptospiral DNA was detected in 2 kidney samples (3.64%). Leptospira were identified through MLST as L. borgpetersenii serovar Tarassovi (serogroup Tarassovi). Obtained data confirmed the presence of Leptospira infection among pigs in southern Italy, suggesting that management of these animals may be considered an occupational risk for humans.

Bovine besnoitiosis is a parasitic disease caused by the protozoan Besnoitia besnoiti, leading to infertility in bulls and abortions in cows. In Italy, it is considered an emerging disease, recently introduced by the importation of animals from Spain and France. In the last decade, many outbreaks have been reported and confirmed in native cattle, mostly in northern and central Italy. This study reports on an autochthonous outbreak of bovine besnoitiosis in two nearby farms located in northwestern Sicily. A 15-month-old Limousine bull born on the farm showed typical clinical signs of the chronic disease phase, such as edema of the scrotum with subsequent hyperkeratosis associated with eschars and thickening of the skin. A histopathological examination revealed the presence of Besnoitia tissue cysts containing bradyzoites in the eyes, tendons, testicles, dermis, and nictitating membrane. A serological investigation using a commercial ELISA kit revealed a high seroprevalence of the antibody anti-B. besnoiti (79.2% for the farms in this study). Clinical disease showed low prevalence (1.5%) despite the high seroprevalence of specific antibodies in the herd, confirming that bovine besnoitiosis is an emergent endemic pathogen in Sicily, but its clinical behavior still remains sporadic.

Pseudorabies virus (PrV) is the etiological agent of Aujeszky’s disease, a viral infection that causes neurological lethal illness in mammals other than swine. Herein, we describe the occurrence of PrV infection in a hunting dog that had been bitten by an infected wild boar in Sicily, reporting for the first time genetic and phylogenetic data on the virus strain isolated in a dog in this Italian region. The dog was referred for severe neurological signs, respiratory distress, and intense itch around the muzzle. Death occurred within 48 h to the onset of clinical signs. On gross examination, self-induced skin lesions to the head due to intense itching and diffuse cerebral congestion were observed, whereas mild, aspecific, nonsuppurative meningitis was histologically diagnosed. Diffuse PrV positivity in neurons of the brainstem was observed by immunohistochemistry. PrV DNA was isolated and amplified from olfactory bulbs by nested PCR, targeting the viral glycoprotein G gene, and the sequence obtained matched with sequences of PrV isolates from dogs and wild boar. Isolation of PrV in the dog herein analysed denotes the spread of the virus in wild boar populations in Sicily and provides a proof of direct interspecies transmission. Thus, there is an urgent need to increase our understanding of the epidemiology of the PrV infection in wildlife to provide tools to trace possible spill over into domestic pigs or other livestock.

This study aimed to investigate parasite fauna of E. marginatus from the central Mediterranean Sea between Messina and Syracuse. In the present survey; parasite fauna of dusky grouper was investigated for two main reasons: the economic value of this species and the current lack of studies regarding the capture area. Seventy dusky groupers were caught from May 2018 to February 2020. Forty-seven out of the 70 specimens (67.2%) were infected with one or more parasite species. The most abundant species was Prosorhynchus caudovatus (42.9%), followed by Podocotyle temensis (28.6%), Didymodiclinus sp. (18.6%), Philometra jordanoi (5.7%), Anisakis Type II larvae (5.7%). Higher prevalence of infection of P. jordanoi and Contracaecum sp. was found in warm months (March to September), while P. caudovatus and P. temensis were mostly found during cold months. Weight and total length of E. marginatus were positively correlated with the parasitic load of P. jordanoi and Didymodiclinus sp. The different prevalence of parasite infection found between warm and cold months is probably related to the diet of the dusky grouper; which is characterized by mollusks that are intermediate hosts for parasite species found. None of the parasites found in the present survey is responsible for zoonosis

In a project designed to investigate the specific and infraspecific taxa of Matthiola endemic to Sicily (Italy) as new potential sources of bioactive compounds in this work, the infraspecific taxa of Matthiola fruticulosa were studied, namely, subsp. fruticulosa and subsp. coronopifolia. HPLC–PDA/ESI–MS and SPME–GC/MS analyses of hydroalcoholic extracts obtained from the aerial parts of the two subspecies led to the detection of 51 phenolics and 61 volatile components, highlighting a quite different qualitative–quantitative profile. The antioxidant properties of the extracts were explored through in vitro methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power and Fe2+ chelating activity assays. The results of the antioxidant tests showed that the extracts possess a different antioxidant ability: particularly, the extract of M. fruticulosa subsp. fruticulosa exhibited higher radical scavenging activity than that of subsp. coronopifolia (IC50 = 1.25 ± 0.02 mg/mL and 2.86 ± 0.05 mg/mL), which in turn displayed better chelating properties (IC50 = 1.49 ± 0.01 mg/mL and 0.63 ± 0.01 mg/mL). Lastly, Artemia salina lethality bioassay was performed for toxicity assessment. The results of the bioassay showed lack of toxicity against brine shrimp larvae for both extracts. The data presented indicate the infraspecific taxa of M. fruticulosa as new and safe sources of antioxidant compounds.

Climate change, food security, and the protection of the planet’s resources require the adoption of sustainable production models. Achieving sustainable development in the agri-food sector enables the creation of new opportunities for operators, guiding farmers towards more environmentally friendly practices and offering cost-effective results. Organic farming paradigms are promoted by the transformation of some harmful practices of conventional agriculture, such as the wide use of chemical products of synthesis, the deep workings that favor the erosive processes, the excessive use of nitrogenous fertilizers. There are still gaps in the knowledge of the real performance of some products that strongly support the local economic system of Sicily (Italy). The research aims to highlight the differences in environmental impact caused by the cultivation of organic early potatoes compared to the conventional regime and the same per kg of product obtained. To this end, the widely used methodology for comparing the environmental impacts of agricultural production systems is the Life Cycle Assessment, which allows us to highlight the phases in which environmental criticalities are most concentrated. An interesting agroecological picture of knowledge emerges, since organic farming is by definition an ecological model that supports the principles of the Green Deal, it often requires interventions to improve the yields obtained in order to achieve a positive result both in terms of cultivated surface and kg of product obtained.

The global sea-level rise (SLR) projections for the next few decades are the basis for developing flooding maps that depict the expected hazard scenarios. However, the spatially variable land subsidence has generally not been considered in the current projections. In this study, we use geodetic data from global navigation satellite system (GNSS), synthetic aperture radar interferometric measurements (InSAR) and sea-level data from tidal stations to show the combined effects of land subsidence and SLR along the coast between Catania and Marzamemi, in south-eastern Sicily (southern Italy). This is one of the most active tectonic areas of the Mediterranean basin, which drives accelerated SLR, continuous coastal retreat and increasing effects of flooding and storms surges. We focus on six selected areas, which show valuable coastal infrastructures and natural reserves where the expected SLR in the next few years could be a potential cause of significant land flooding and morphological changes of the coastal strip. Through a multidisciplinary study, the multi-temporal flooding scenarios until 2100, have been estimated. Results are based on the spatially variable rates of vertical land movements (VLM), the topographic features of the area provided by airborne Light Detection And Ranging (LiDAR) data and the Intergovernmental Panel on Climate Change (IPCC) projections of SLR in the Representative Concentration Pathways RCP 2.6 and RCP 8.5 emission scenarios. In addition, from the analysis of the time series of optical satellite images, a coastal retreat up to 70 m has been observed at the Ciane river mouth (Siracusa) in the time span 2001–2019. Our results show a diffuse land subsidence locally exceeding 10 ± 2.5 mm/year in some areas, due to compacting artificial landfill, salt marshes and Holocene soft deposits. Given ongoing land subsidence, a high end of RSLR in the RCP 8.5 at 0.52 ± 0.05 m and 1.52 ± 0.13 m is expected for 2050 AD and 2100 AD, respectively, with an exposed area of about 9.7 km2 that will be vulnerable to inundation in the next 80 years.

Oxygen isotopic composition is useful for individuating recharge areas of groundwater bodies by the comparison with those of local rainfalls. While on a global scale general relationships, such as the isotopic vertical gradient or continentality effects, efficiently describe spatial variations of the isotopic signature, hydrogeological applications need spatial models that are more focused on the effects of local topographic structures and/or subsoil geology. This work presents a case study in northeastern Sicily (Italy) characterized by complex geological and orographic structures, in which isotopic composition of rainfalls is governed by orographic effects and the varying initial composition of humid air masses. We used a black box approach, comparing the average isotopic composition of rain collected from a network of eight samplers with their spatial descriptors (elevation, latitude and longitude). We obtained the best correlation with the simultaneous use of all these variables, applying their multiple linear correlation equation to transform the 1 × 1 km digital elevation model (DEM) of the study area into a digital isotopic model (DIM). The reliability of the DIM was confirmed by its good agreement with the oxygen isotopic composition contour map of the local groundwater.

Tropical theileriosis is a tick-borne disease caused by hemoprotozoan parasites with considerable veterinary and economic impact worldwide. Ticks transmitting the disease belong to the Haemaphysalis, Rhipicephalus, and Hyalomma genera. The Hyalomma genus is very common in Sicily (Italy) and represents the main Theileria annulata vector in the island. Data concerning the molecular epidemiology of this pathogen are missing in the region. In 2018–2019, blood and serum samples were collected from 480 cows in seven Sicilian farms from four different provinces. Seroprevalence in the farms ranged from 22% to 71%. Three farms were selected for molecular analysis consisting of real-time PCR targeting the almost complete 18S ribosomal RNA (rRNA). Four amplicons per farm were sequenced and phylogenetic analyses were carried out. The four sequences were identical within each farm and showed 92–99% identity with the other farms and with sequences from Genbank. According to the phylogenetic analysis, these three sequences and an additional one from a laboratory-cultured Theileria annulata strain obtained in 1999 belonged to a single T. annulata clade with good bootstrap support with other sequences from Italy, India, and Iran, indicating limited geographical and temporal genetic variability of the parasite. This study represents the first phylogenetic analysis of T. annulata in Sicily, which will be useful to improve the strategies for theileriosis control and prevention.

The red gum lerp psyllid, Glycaspis brimblecombei Moore (Hemiptera: Aphalaridae), is an invasive pest of Eucalyptus trees worldwide, responsible for serious damage, including the death of plants. Knowledge about the incidence of climatic factors on the insect development are essential to define useful strategies for controlling this pest. To this aim, G. brimblecombei has been sampled by two different methods from April 2012 to February 2013 in eastern Sicily on Eucalyptus camaldulensis in nine different sites, where the main climatic data (air temperature, relative humidity, and precipitation) have been also registered. The Glycaspis brimblecombei population showed a similar trend in all nine sites, positively correlated only with air temperature, but a negative correlation has emerged with precipitation and relative humidity. The results show the need for a deeper understanding of the role played by other abiotic (such as different concentrations of CO2) and biotic (e.g., the antagonistic action of natural enemies, competition with other pests, etc.) factors. The greater sensitivity, even at low densities of psyllid, of sampling methods based on the random collection of a fixed number of leaves compared to methods based on the collection of infested leaves in a fixed time interval has been also outlined.

Oregano is—probably—the most appreciated and widespread aromatic plant in Sicily. With the aim of evaluating the modifications of oregano’s essential oil composition over time, between 2013 and 2015 six weekly samplings of three different oregano plantations were carried out, from the beginning of flowering (early May) until the traditional harvest moment (end of June). Samples were hydrodistilled and the obtained essential oils (EOs) were evaluated by means of a combination of GC–FID and GC–MS. The Origanum plants under study were demonstrated to belong to the high-yielding, thymol-type biotypes, with thymol, γ-terpinene and p-cymene as three main components, among the total of about 50 of the evaluated EOs. In each location, EO yields were found to increase throughout survey dates. Significant variations were found in many EO components, both across years and throughout harvest dates within locations. The choice of the harvest moment was confirmed to be crucial in assessing quality aspects of oregano.

Cotton is one of the most important industrial crops in the world. Though widely cultivated in Sicily (Italy) in the past, cotton growth on the island has disappeared today due to a complex variety of agronomic, economic and socio-political reasons. In recent years, increased interest in natural fibers worldwide has led to a revival in cotton plants in the Mediterranean area. The aim of this paper was to assess the response of Gossypium hirsutum L. cultivars to different environments and sowing times. Elsa and Juncal were selected from the most promising cotton cultivars regarding earliness and productivity. Plants were tested with three sowing times and in two Sicilian environments. Cotton yield and yield components were significantly affected by experimental station, sowing time and cultivar. Lint yield of cultivars was 1.60 t ha−1 on average, and the highest value of 1.99 t ha−1 was obtained from an early sowing time. The three indices of agronomic earliness varied significantly based on treatments. In conclusion, the evaluation of response genotype-by-environment under different sowing times could represent a strategy to obtain optimal cotton seed and lint yields, although other general aspects, such as labor costs, land availability and capital resources, should be also considered when evaluating the reintroduction of the species in Sicily.

Geochemical characteristics of middle ocean ridge basalts (MORBs) testify partial melting of spinel-peridotite mixed with a few amounts of garnet-pyroxenite. The latter can be considered either autochthonous products of the crystallization of partial melts in the sub-oceanic mantle or allocthonous recycled crustal materials originated in subduction contexts. Here we suggest the “autocthnous recycled” origin for garnet-pyroxenites. Such a hypothesis derives from the study of garnet-bearing pyroxenite xenoliths from the Hyblean Plateau (Sicily). These consist of Al-diopside, pyralspite-series garnet, Al-spinel and Al-rich orthopyroxene. Trace element distribution resembles an enriched MORB but lower chromium. Major-element abundances closely fit in a tschermakitic-horneblende composition. Assuming that a high-Al amphibolite was formed by hydrothermal metasomatism of a troctolitic gabbro in a slow-spreading ridge segment, a transient temperature increasing induced dehydroxilization reaction in amphiboles, giving Al-spinel-pyroxenite and vapor as products. Garnet partially replaced spinel during an isobaric cooling stage. Density measurements at room conditions on representative samples gave values in the range 3290–3380 kg m−3. In general, a density contrast ≥300 kg m−3 can give rise to convective instability, provided a sufficient large size of the heavy masses and adequate rheological conditions of the system. Garnet-pyroxenite lumps can therefore sink in the underlying mantle, imparting the “garnet geochemical signature” to newly forming basaltic magma.

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation of the Sicilian wetlands played a major role in this process. Finally, some conservation and management suggestions aimed at preventing the loss of genetic variability of the species are briefly reported, stressing the importance of considering the five detected clusters as independent Management Units.

In the Mediterranean Sea, the coralligenous is an extremely important habitat for its biodiversity and role in carbon sequestration. However, coralligenous biocenosis is sensitive to many anthropogenic impacts, among which one of the major threats is the marine litter. The aim of the present work was to evaluate the marine litter present in the coralligenous habitat of the Marine Protected Area (MPA) Isole Ciclopi (Sicily, Italy). Through the analysis of frames obtained by Remotely Operated Vehicles (ROV) videos, data on the type of marine litter, grade of organisms’ coverage, litter-organism interactions, and seabed coverage of items were gained. Through ROV surveys, a low number of marine litter items was observed and most of them were fishing gears. On the found items there was a high degree of organisms’ coverage, which suggests that probably the marine litter was abandoned or lost since a long time. Overall, it was observed that in recent years the fishing activity in the MPA has not affected the coralligenous habitat. The present study represents a baseline for future monitoring programmes, which will be useful to develop protection measures and sustainable fishing, in order to preserve the coralligenous habitat of the MPA.

Post-mortem inspection in slaughterhouses plays a key role in the epidemiological surveillance of infectious diseases, including bTB. This study assessed the prevalence of bTB in cattle in Sicily during two different three-year periods (2010–2012; 2017–2019), using data from meat inspections and histopathological methods. Out of 100,196 cattle, 5221 (5.21%) were diagnosed with tuberculous lesions. Higher prevalence of bTB was recorded during the triennium 2010–2012 (6.74%; n = 3692) compared to the triennium 2017–2019 (3.36%; n = 1529), with a decreasing trend in annual occurrence throughout the study period and a heterogenous proportion of infected cattle among the Sicilian provinces (p < 0.01). Lower rates of infection were recorded in animals aged 84 months (p < 0.0001). Pearson’s chi square analysis revealed a higher localization of lesions in the thoracic cavity (p < 0.0001). Gathered findings show that even though bTB occurrence in cattle was greatly reduced over the years, the disease still remains widespread in Sicily, also posing severe implications for public health.

Forward logistic regression and conditional analysis have been compared to assess landslide susceptibility across the whole territory of the Sicilian region (about 25,000 km2) using previously existing data and a nested tiered approach. These approaches were aimed at singling out a statistical correlation between the spatial distribution of landslides that have affected the Sicilian region in the past, and a set of controlling factors: outcropping lithology, rainfall, landform classification, soil use, and steepness. The landslide inventory used the proposal of building the models like the official one obtained in the PAI (hydro geologic asset plan) project, amounting to more than 33,000 events. The 11 types featured in PAI were grouped into 4 macro-typologies, depending on the inherent conditions believed to generate various kinds of failures and their kinematic evolution. The study has confirmed that it is possible to carry out a regional landslide susceptibility assessment based solely on existing data (i.e., factor maps and the landslide archive), saving a considerable amount of time and money. For scarp landslides, where the selected factors (steepness, landform classification, and lithology) are more discriminate, models show excellent performance: areas under receiver operating characteristic (ROC) (AUCs) average > 0.9, while hillslope landslide results are highly satisfactory (average AUCs of about 0.8). The stochastic approach makes it possible to classify the Sicilian territory depending on its propensity to landslides in order to identify those municipalities which are most susceptible at this level of study, and are potentially worthy of more specific studies, as required by European-level protocols.

Background : Reinfections occur as a response to natural infections wanes and novel strains of SARS-CoV-2 emerge. The present research explored the correlation between sex, age, COVID-19 vaccination, prior infection hospitalization, and SARS-CoV-2 reinfection in Sicily, Italy. Materials and Methods : A population-based retrospective cohort study was articulated using the vaccination flux from a regional registry and the Sicilian COVID-19 monitoring system of the Italian Institute of Health. Only adult Sicilians were included in the study, and hazard ratios were calculated using Cox regression. Results : Partial vaccination provided some protection (adj-HR: 0.92), when compared to unvaccinated individuals; furthermore, reinfection risk was reduced by full vaccination (adj-HR: 0.43), and the booster dose (adj-HR: 0.41). Males had a lower risk than females of reinfection with SARS-CoV-2 (adj-HR: 0.75). Reinfection with SARS-CoV-2 was diminished by hospitalization during the first infection (adj-HR: 0.78). Reinfection risk was higher among those aged 30-39 and 40-49 compared to those aged 18-29, whereas those aged 60-69, 70-79, and 80+ were statistically protected. Reinfection was significantly more frequent during the wild-type-Alpha, Delta, Delta-Omicron, and Omicron dominance/codominance waves compared to the wild type. Conclusions : This study establishes a solid base for comprehending the reinfection phenomenon in Sicily by pinpointing the most urgent policy hurdles and identifying some of the major factors. COVID-19 vaccination, one of the most effective public health tools, protects against reinfection, mostly caused by the Omicron strain. Elderly and hospitalized people's lower risk suggests stricter PPE use.

Interest in oilseed crops for agro-industrial research and development projects has increased in the Mediterranean area, in recent years. Safflower (Carthamus tinctorius L.) is of potential interest for agriculture mainly due to fatty acid content variability in the seed oil. The aim of this study was to assess the agronomic performance of 16 new safflower accessions together with safflower variety Montola 2000, used as a reference, in a semi-arid environment. Research was carried out in Sicily (Italy) from 2013−2014. Hierarchical cluster analysis carried out on the fatty acid composition of safflower accessions resulted in their division into four main groups. Linoleic, oleic and palmitic acids were the main fatty acids present in the accessions. Seed yield was 1.11 t ha−1 on average and seed oil content was found to be approximately 35.01% of dry matter on average. Positive and significant relationships between seed/oil yield and other tested traits were found. The carbon, hydrogen and nitrogen content as a percentage of dry matter varied greatly both for the above- and belowground plant parts on average. This study confirms the interest of safflower for both food and non-food applications, offering interesting prospects in semi-arid regions.

Built in the second to early-first century BCE, the House of Leda at Soluntum, a city on the northwest coast of Sicily, was renovated in the first century CE. The most prominent change to the residence was the inclusion of figural Fourth-Style wall paintings in its dining room. The fresco ensemble reveals a particular interest in the painted depiction of stone, such as an image of Leda and the swan as a marble statue and trompe l’oeil blocks of colored marble and granite from around the Mediterranean. The house renovation was not wholesale since the owner also chose to preserve a number of decorative elements from the earlier, Hellenistic-era phase of the residence, including two sculptures, cut-limestone pavements, and an intricate mosaic of an astronomical instrument. In this article I argue that the tension created between the medium of paint, and its use to mimic marble and stone, resulted in a unified, mixed-media domestic ensemble. The viewer was encouraged to compare and contrast the faux marble and stone in the dining room’s Fourth-Style frescoes with the Hellenistic-era marble and stone artworks throughout the rest of the house. This juxtaposition of older and newer decorative elements reveals that the owner of the House of Leda positioned himself as both a member of the Roman provincial elite as well as a local benefactor and custodian of Sicily’s rich Hellenistic culture.

Airborne laser scanning (ALS) data can provide more than just a topographic data set for archaeological research. During data collection, laser scanning systems also record radiometric information containing object properties, and thus information about archaeological features. Being aware of the physical model of ALS scanning, the radiometric information can be used to calculate material information of the scanned object. The reflectance of an object or material states the amount of energy it reflects for a specific electromagnetic wavelength. However, the collected radiometric data are affected by several factors that cause dissimilar values to be recorded for the same object. Radiometric calibration of such data minimizes these differences in calculated reflectance values of objects, improving their usability for feature detection and visualization purposes. Previous work dealing with calibration of radiometric data in archaeological research has relied on corresponding in-field measurements to acquire calibration values or has only corrected for a limited number of variables. In this paper, we apply a desk-based approach in which radiometric calibration is conducted through the selection of homogenous areas of interest, without the use of in-field measurements. Together with flight and scan parameters, radiometric calibration allows for the estimation of reflectance values for returns of a single full-waveform ALS data collection flight. The resulting data are then processed into a raster reflectance map that approximates a monochromatic illumination-independent true orthoimage at the wavelength of the laser scanner. We apply this approach to data collected for an archaeological research project in western Sicily and discuss the relative merits of the uses of radiometric data in such locations as well as its wider applicability for present and future archaeological and environmental research. In order to make the approach more accessible, we have developed a freely available tool that allows users to apply the calibration procedure to their own data.

Stringent environmental constraints make the construction of new transmission overhead lines more and more difficult. Alternatively, today it is possible to use cable lines for high (HV) and extra-high (EHV) voltage systems. The configuration of the so-called mixed lines can create some problems in the operation of the electrical system, both during steady-state and transient conditions. In particular, the system stability is one of the main concerns when analyzing the dynamic response of power systems. In this paper, the study of angular stability of a system containing a mixed line is presented: a specific control logic applied to the shunt reactors of the mixed line is proposed as improvement of the overall system stability. The proposed switching logic is first discussed from a theoretical point of view and validated with two different testing systems. Then, the existing overhead-cable lines connecting Sicily to the rest of continental Europe 400 kV power system are taken as case study for the application of the proposed switching strategy. Several simulations are performed in the power system analysis software NEPLAN360: the results show the fundamental role of the timing of the control actions applied on the shunt reactors in helping the system to keep the stability. The proposed control proves to be an effective support to the system subjected to critical contingencies, contributing decisively to avoid the angular separation between areas and therefore to preserve the stability of the system.

Human respiratory syncytial virus (hRSV) is an important pathogen of acute respiratory tract infection of global significance. In this study, we investigated the molecular epidemiology and the genetic variability of hRSV over seven surveillance seasons between 2015 and 2023 in Sicily, Italy. hRSV subgroups co-circulated through every season, although hRSV-B mostly prevailed. After the considerable reduction in the circulation of hRSV due to the widespread implementation of non-pharmaceutical preventive measures during the COVID-19 pandemic, hRSV rapidly re-emerged at a high intensity in 2022-2023. The G gene was sequenced for genotyping and analysis of deduced amino acids. A total of 128 hRSV-A and 179 hRSV-B G gene sequences were obtained. The phylogenetic analysis revealed that the GA2.3.5a (ON1) and GB5.0.5a (BA9) genotypes were responsible for the hRSV epidemics in Sicily.; only one strain belonged to the genotype GB5.0.4a. No differences were observed in the circulating genotypes during pre- and post-pandemic years. Amino acid sequence alignment revealed the continuous evolution of the G gene, with a combination of amino acid changes specifically appearing in 2022-2023. The predicted N-glycosylation sites were relatively conserved in ON1 and BA9 genotype strains. Our findings augment the understanding and prediction of the seasonal evolution of hRSV at the local level and its implication in the monitoring of novel variants worth considering in better design of candidate vaccines.

The Quaternary mammals of Sicily are well known, and five faunal complexes have been distinguished on the basis of bioevents (extinctions and new arrivals) and evolution of endemic species. It is clear that the composition of mammal faunas is strictly related to the dispersal ability of each species and to the paleogeography of the area. Until now, researches have chiefly attributed paleogeographical changes as controlling these dispersals: the sea strait between the island and the Italian peninsula has had different widths and depths over time, operating different kinds of filters on the spreading of terrestrial mammals. Moreover, Sicily and its nearby mainland underwent changes in paleogeography. Some incongruence in bioevents has been attributed to the filter operated by the marine strait, which could have acted in differential ways on large and small mammals. However, the roles of ecological interactions among vertebrate species and their control on bioevents have been greatly underestimated. In this critical review, changes in mammals’ associations are reconsidered not only in terms of biochronology and dispersal ability of taxa through the marine strait, evolution of endemic features, in addition to the paleogeography of the island, but also considering the ecological role of each species and the interactions among the species with each faunal complex.

Two new fossil species of Ostracoda belonging to the genus Bythocythere Sars, 1866, Bythocythere solisdeus n. sp. and to the genus Cytheropteron Sars, 1866, Cytheropteron eleonorae n. sp. are described. The specimens come from the upper silty sand layers of the Globorotalia truncatulinoides excelsa Zone (“Sicilian” stage), cropping out in “Cala S. Antonino” along the western side of the Cape Milazzo Peninsula (NE Sicily). Both species belong to a typical Bathyal ostracod association characterized by very low temperatures.

Malta and Sicily, which lie at the centre of the Mediterranean Sea, share a long history and have unique geological and geomorphological features which make them attractive destinations for geotourism. In the framework of an international research project, a study for the identification, selection and assessment of the rich geological heritage of Malta and Sicily was carried out, aiming to create a geosite network between these islands. Based on the experience and outputs achieved in previous investigations on geoheritage assessment carried out in various morpho-climatic contexts, an integrated methodology was applied for the selection, numerical assessment and ranking of geosites. The selection phase was based on three main criteria—scientific, additional and use values—and led to the establishment of a list of 42 geosites (20 in Malta and 22 in Sicily). Besides being spectacular and attractive for tourists, these sites represent the main geomorphological contexts and the various stages of regional morphogenesis of the study areas. The sites selected were assessed quantitatively and ranked according to management and tourism criteria. The results provide both the necessary basic knowledge for joint conservation actions and policies in Malta and Sicily and the elements for creating a link between Malta and Sicily through geoheritage appraisal and tourism development.