1. Development and Application of a TaqMan RT-qPCR for the Detection of Foot-and-Mouth Disease Virus in Pigs
- Author
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Changying Dong, Xingyu Xiao, Meiqi Wang, Yajuan Sun, Hui Jin, Yongzhe Zhang, Hongri Zhao, Qianyue Cao, Yanran Yang, and Rui Yin
- Subjects
foot-and-mouth disease virus ,TaqMan real-time RT-qPCR ,system optimization ,detection methods ,Veterinary medicine ,SF600-1100 - Abstract
The global livestock industry is facing a serious threat from a widespread foot-and-mouth disease virus (FMDV) epidemic. The timely detection of FMDV can significantly mitigate its harmful effects. This study aimed to establish and evaluate a TaqMan fluorescence quantitative PCR assay to assess its sensitivity, specificity, reproducibility, and stability. The standard curve equation range is 6.43 × 109–6.43 × 101 copies/µL, with an R2 value of 0.996 and a standard curve equation of y = −3.586x + 36.245. The method successfully detected 64.3 copies/µL of the target gene for FMDV and exhibited high specificity for FMDV. Repeatability tests demonstrated low coefficients of variation within and between groups (
- Published
- 2024
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