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To address the subjectivity of traditional factor attribute grading methods and the weak predictive capabilities of single-model classifications, this study focused on Yulong County; the Contribution Degree Clustering Method (CDCM) utilizes the Certainty Factor (CF) as the contribution index to partition continuous factor attribute intervals. Additionally, the Sparrow Search Optimization Algorithm (SSA) is employed for hyperparameter tuning. The CF is incorporated into Support Vector Machine (SVM), Back Propagation Neural Network (BPNN), and Random Forest (RF) models to form the CF-SSA-SVM, CF-SSA-BPNN, and CF-SSA-RF coupling models, respectively. These basic coupling models are further integrated using the Stacking algorithm to create the CF-SSA-Stacking integrated coupling model for constructing a landslide susceptibility assessment system. The results indicate that the CF-SSA-Stacking integrated coupling model achieves the highest accuracy, F1 score, Kappa coefficient, and AUC value, with values of 0.89375, 0.89172, 0.787500, and 0.9522, respectively. These metrics are significantly superior to those of the three basic coupling models, demonstrating better generalization capability and reliability. This suggests that the model can identify more historical landslide occurrences using fewer grid areas classified as extremely-high- or high-susceptibility zones. It is suitable as an effective regional landslide susceptibility assessment method for practical disaster prevention and mitigation applications. Further studies could explore the model’s performance across varying geological settings or with different datasets, providing a roadmap for future research and development in landslide susceptibility assessment.

As the competition intensifies in enhancing the integration and performance of integrated circuits, in accordance with the famous Moore’s Law, higher performance and smaller size requirements are imposed on the dielectric layers in electronic devices. Compared to vacuum methods, the production cost of preparing dielectric layers via solution methods is lower, and the preparation cycle is shorter. This paper utilizes a low-temperature self-exothermic reaction based on the solution method to prepare high-performance Al2O3 dielectric thin films that are compatible with flexible substrates. In this paper, we first established two non-self-exothermic systems: one with pure aluminum nitrate and one with pure aluminum acetylacetonate. Additionally, we set up one self-exothermic system where aluminum nitrate and aluminum acetylacetonate were mixed in a 1:1 ratio. Tests revealed that the leakage current density and dielectric constant of the self-exothermic system devices were significantly optimized compared to the two non-self-exothermic system devices, indicating that the self-exothermic reaction can effectively improve the quality of the dielectric film. This paper further established two self-exothermic systems with aluminum nitrate and aluminum acetylacetonate mixed in 2:1 and 1:2 ratios, respectively, for comparison. The results indicate that as the proportion of aluminum nitrate increases, the overall dielectric performance of the devices improves. The best overall performance occurs when aluminum nitrate and aluminum acetylacetonate are mixed in a ratio of 2:1: The film surface is smooth without cracks; the surface roughness is 0.747 ± 0.045 nm; the visible light transmittance reaches up to 98%; on the basis of this film, MIM devices were fabricated, with tested leakage current density as low as 1.08 × 10−8 A/cm2 @1 MV and a relative dielectric constant as high as 8.61 ± 0.06, demonstrating excellent electrical performance.

Geohazards that have developed in densely vegetated alpine gorges exhibit characteristics such as remote occurrence, high concealment, and cascading effects. Utilizing a single remote sensing datum for their identification has limitations, while utilizing multiple remote sensing data obtained based on different sensors can allow comprehensive and accurate identification of geohazards in such areas. This study takes the Latudi River valley, a tributary of the Nujiang River in the Hengduan Mountains, as the research area, and comprehensively uses three techniques of remote sensing: unmanned aerial vehicle (UAV) Light Detection and Ranging (LiDAR), Small Baseline Subset interferometric synthetic aperture radar (SBAS-InSAR), and UAV optical remote sensing. These techniques are applied to comprehensively identify and analyze landslides, rockfalls, and debris flows in the valley. The results show that a total of 32 geohazards were identified, including 18 landslides, 8 rockfalls, and 6 debris flows. These hazards are distributed along the banks of the Latudi River, significantly influenced by rainfall and distribution of water systems, with deformation variables fluctuating with rainfall. The three types of geohazards cause cascading disasters, and exhibit different characteristics in the 0.5 m resolution hillshade map extracted from LiDAR data. UAV LiDAR has advantages in densely vegetated alpine gorges: after the selection of suitable filtering algorithms and parameters of the point cloud, it can obtain detailed terrain and geomorphological information on geohazards. The different remote sensing technologies used in this study can mutually confirm and complement each other, enhancing the capability to identify geohazards and their associated hazard cascades in densely vegetated alpine gorges, thereby providing valuable references for government departments in disaster prevention and reduction work.

Villosiclava virens (anamorph: Ustilaginoidea virens) is the pathogen of rice false smut (RFS), which is a destructive rice fungal disease. The albino strain LN02 is a natural white-phenotype mutant of V. virens due to its incapability to produce toxic ustilaginoidins. In this study, three strains including the normal strain P1, albino strain LN02, and complemented strain uvpks1C-1 of the LN02 strain were employed to investigate the activation of the ustilaginoidin biosynthesis gene uvpks1 in the albino strain LN02 to influence sporulation, conidia germination, pigment production, stress responses, and the inhibition of rice seed germination. The activation of the ustilaginoidin biosynthesis gene uvpks1 increased fungal tolerances to NaCl-induced osmotic stress, Congo-red-induced cell wall stress, SDS-induced cell membrane stress, and H2O2-induced oxidative stress. The activation of uvpks1 also increased sporulation, conidia germination, pigment production, and the inhibition of rice seed germination. In addition, the activation of uvpks1 was able to increase the mycelial growth of the V. virens albino strain LN02 at 23 °C and a pH from 5.5 to 7.5. The findings help in understanding the effects of the activation of uvpks1 in albino strain LN02 on development, pigment production, stress responses, and the inhibition of rice seed germination by controlling ustilaginoidin biosynthesis.

Solution-processed cadmium telluride (CdTe) nanocrystal (NC) solar cells offer the advantages of low cost, low consumption of materials and large-scale production via a roll-to-roll manufacture process. Undecorated CdTe NC solar cells, however, tend to show inferior performance due to the abundant crystal boundaries within the active CdTe NC layer. The introduction of hole transport layer (HTL) is effective for promoting the performance of CdTe NC solar cells. Although high-performance CdTe NC solar cells have been realized by adopting organic HTLs, the contact resistance between active layer and the electrode is still a large problem due to the parasitic resistance of HTLs. Here, we developed a simple phosphine-doping technique via a solution process under ambient conditions using triphenylphosphine (TPP) as a phosphine source. This doping technique effectively promoted the power conversion efficiency (PCE) of devices to 5.41% and enabled the device to have extraordinary stability, showing a superior performance compared with the control device. Characterizations suggested that the introduction of the phosphine dopant led to higher carrier concentration, hole mobility and a longer lifetime of the carriers. Our work presents a new and simple phosphine-doping strategy for further improving the performance of CdTe NC solar cells.

Sorbicillinoids are a class of hexaketide metabolites produced by Ustilaginoidea virens (teleomorph: Villosiclava virens), an important fungal pathogen that causes a devastating rice disease. In this study, we investigated the effects of environmental factors, including carbon and nitrogen sources, ambient pH and light exposure, on mycelial growth, sporulation, as well as the accumulation of sorbicillinoids, and the expression of related genes involved in sorbicillinoid biosynthesis. It was found that the environmental factors had great influences on mycelial growth and sporulation of U. virens. Fructose and glucose, complex nitrogen sources, acidic conditions and light exposure were favorable for sorbicillinoid production. The relative transcript levels of sorbicillinoid biosynthesis genes were up-regulated when U. virens was separately treated with those environmental factors that favored sorbicillinoid production, indicating that sorbicillinoid biosynthesis was mainly regulated at the transcriptional level by different environmental factors. Two pathway-specific transcription factor genes, UvSorR1 and UvSorR2, were found to participate in the regulation of sorbicillinoid biosynthesis. These results will provide useful information to better understand the regulation mechanisms of sorbicillinoid biosynthesis, and be conducive to develop effective means for controlling sorbicillinoid production in U. virens.

Upland cotton (Gossypium hirsutum L.) is one of the important fiber crops. Cotton flowers usually appear white (or cream-colored) without colored spots at the petal base, and turn pink on the next day after flowering. In this study, using a mutant showing pink petals with crimson spots at their base, we conducted comparative metabolome and transcriptome analyses to investigate the molecular mechanism of coloration in cotton flowers. Metabolic profiling showed that cyanidin-3-O-glucoside and glycosidic derivatives of pelargonidins and peonidins are the main pigments responsible for the coloration of the pink petals of the mutant. A total of 2443 genes differentially expressed (DEGs) between the white and pink petals were identified by RNA-sequencing. Many DEGs are structural genes and regulatory genes of the anthocyanin biosynthesis pathway. Among them, MYB21, UGT88F3, GSTF12, and VPS32.3 showed significant association with the accumulation of cyanidin-3-O-glucoside in the pink petals. Taken together, our study preliminarily revealed the metabolites responsible for the pink petals and the key genes regulating the biosynthesis and accumulation of anthocyanins in the pink petals. The results provide new insights into the biochemical and molecular mechanism underlying anthocyanin biosynthesis in upland cotton.

ACO is one of the rate-limiting enzymes in the biosynthesis of ethylene, and it plays a critical role in the regulation of plant growth and development. However, the function of ACO genes in cotton is not well studied. In this study, a total of 332 GhACOs, 187 GaACOs, and 181 GrACOs were identified in G. hirsutum, G. arboretum, and G. raimondii, respectively. Gene duplication analysis showed that whole-genome duplication (WGD) and tandem duplication were the major forces driving the generation of cotton ACO genes. In the promoters of GhACOs, there were cis-acting elements responding to stress, phytohormones, light, and circadian factors, indicating the possible involvement of GhACOs in these processes. Expression and co-expression analyses illustrated that most GhACOs were not only widely expressed in various tissues but also coexpressed with other genes in response to salt and drought stress. GhACO106_At overexpression in Arabidopsis promoted flowering and increased salt tolerance. These results provide a comprehensive overview of the ACO genes of cotton and lay the foundation for subsequent functional studies of these genes.

Water samples from rainfall, river, springs, and wells in the Xianshui River fault region near Xialatuo, China were collected during two sampling campaigns to investigate the complex groundwater circulation in the region. The major ions, stable isotopes, and four natural radium isotopes of the water samples were analyzed, and the results were utilized to identify different groundwater circulation depths. Most water samples excluding the one at a hot spring and the one at a borehole possess similar hydrochemical compositions and lower total dissolved solids (TDS), implying that their circulation depth is relatively shallow or that residence time is short. The sample at the hot spring has high TDS and high temperature as well as the high F concentration, inferring that it may circulate at a deeper depth. The sample at the borehole contains mixed hydrochemical characteristics of other samples. Three groundwater flow systems may exist in the study area: the shallow groundwater system recharged by precipitations and local groundwater flow, the deep groundwater system recharged by the regional groundwater flow, and the intermediate one between the above two systems. The finding of the three flow systems is supported by the δ2H and δ18O as well as the apparent radium ages of the samples. The δ2H and δ18O values at the intercept of the line formed by the shallow groundwater samples and the local meteoric water line (LMWL) are similar to those of modern precipitations. The δ2H and δ18O values at the intercept of the line formed by the deep groundwater samples and the LMWL show that it is probably recharged by relatively older precipitations. The 2H and 18O values of the borehole samples are between the above two intercept points. The deep-circulated groundwater with high temperature has longer apparent radium age than other water samples. The apparent radium ages of the shallow groundwater are similar but less than that of the deep groundwater. Groundwater at the borehole may circulate at a depth between the above two. The results of this study improve our understanding of the complex groundwater circulation and enable us to better protect and manage the groundwater resources in the region.

Hepatitis E virus (HEV) causes predominantly acute and self-limiting hepatitis. However, in HEV-infected pregnant women, the case fatality rate because of fulminant hepatitis can be up to 30%. HEV infection is zoonotic for some genotypes. The HEV genome contains three open reading frames: ORF1 encodes the non-structural polyprotein involved in viral RNA replication; ORF2 encodes the capsid protein; ORF3 encodes a small multifunctional protein. Interferons (IFNs) play a significant role in the early stage of the host antiviral response. In this study, we discovered that the capsid protein antagonizes IFN induction. Mechanistically, the capsid protein blocked the phosphorylation of IFN regulatory factor 3 (IRF3) via interaction with the multiprotein complex consisting of mitochondrial antiviral-signaling protein (MAVS), TANK-binding kinase 1 (TBK1), and IRF3. The N-terminal domain of the capsid protein was found to be responsible for the inhibition of IRF3 activation. Further study showed that the arginine-rich-motif in the N-terminal domain is indispensable for the inhibition as mutations of any of the arginine residues abolished the blockage of IRF3 phosphorylation. These results provide further insight into HEV interference with the host innate immunity.

Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using a monoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.