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Recently, more consumers have reached for plant-based milk substitutes, mainly for health, dietary, and flavour reasons. This study aimed to evaluate the physicochemical and bioactive properties and sensory profile of 10 selected rice drinks available on the EU market. Assessment of the physicochemical characteristics included measurement of soluble solids, pH, osmolality, foaming properties and colour parameters. Analysis of bioactive compounds involved the determination of polyphenolic compounds and antioxidant activity. Based on the manufacturer’s data, the composition and nutritional value of the above-mentioned beverages were also analysed in this study. The tested beverages were characterised by a low fat content of 0.6–1.3 g/100 mL, derived mainly from added sunflower oil, but are a good source of energy (energy value of 37–55 kcal/100 mL), derived mainly from naturally occurring carbohydrates whose content ranged from 8.0–11.0 g/100 mL. The protein content of all rice drinks was shallow, not exceeding 0.5 g/100 mL. Of the ten rice drinks analysed, in only two were vitamins B12 and D added (0.38 μg/100 mL and 0.75 μg/100 mL, respectively) and in only three was calcium (120 mg/100 mL) added. In evaluating the physicochemical characteristics, the drinks showed a pH close to neutral (mean 6.85 ± 0.01). The average soluble solids content was about 11%, which was determined by the carbohydrate content. The osmolality of the beverages averaged 324.73 ± 70.17 mOsm/kg H2O, with four beverages classifiable as hypertonic ones (osmolality > 330 mOsm/kg H2O), three as isotonic ones (with osmolality between 270–330 mOsm/kg H2O), and three as hypotonic beverages (osmolality < 270 mOsm/kg H2O). Only two rice drinks evaluated in this study showed foaming properties. The high whiteness index (81.79 ± 2.55) indicated high white colour saturation of the tested beverages. The rice drinks were characterised by a relatively diverse sensory quality regarding aroma and flavour notes. The overall sensory quality was rated highest for the beverages with the highest sugar content. The tested beverages were characterised by a low content of total polyphenols (average 1.40 ± 0.62 mg GAE/100 mL) and relatively high antioxidant activity (average 418.33 ± 59.65 µM TEAC/100 mL). Based on the research conducted and the analysis of the manufacturer’s data, it can be concluded that the rice drinks studied in this paper can be included in the daily diet, providing a rehydrating beverage that shows free radical-neutralizing properties and provides carbohydrates. At the same time, it should be highlighted that the studied drinks have a low nutritional value and cannot be recommended as milk substitutes due to low protein levels and lack of milk-specific vitamins and minerals.

Calypogeia is a genus of liverworts in the family Calypogeiaceae. The subject of this study was Calypogeia suecica. Samples of the liverwort Calypogeia suecica were collected from various places in southern Poland. A total of 25 samples were collected in 2021, and 25 samples were collected in 2022. Volatile organic compounds (VOCs) from liverworts were analyzed by gas chromatography–mass spectrometry (GC–MS). A total of 107 compounds were detected, of which 38 compounds were identified. The identified compounds were dominated by compounds from the sesquiterpene group (up to 34.77%) and sesquiterpenoids (up to 48.24%). The tested samples of Calypogeia suecica also contained compounds belonging the aromatic classification (up to 5.46%), aliphatic hydrocarbons (up to 1.66%), and small amounts of monoterpenes (up to 0.17%) and monoterpenoids (up to 0.30%). Due to the observed differences in the composition of VOCs, the tested plant material was divided into two groups, in accordance with genetic diversity.

The development of wind energy has been observed for many years. Both construction firms and the scientific world are analyzing new design solutions, atmospheric conditions and the technical performance achieved. The main goal of this research is to evaluate the requirements that have to be met to design wind power stations that would be an optimal fit for the climatic conditions in Poland. This study combines the results of empirical studies on wind velocity distributions with the physical fundamentals of wind power station design. This paper presents modelling of the relationships between wind velocity distributions observed in Poland and technical requirements for wind power stations design. The wind velocities distributions for various locations in Poland are determined and expressed in Weibull distribution parameters. Theoretical computations concerning the dependence of wind power stations as function of wind speed and air’s physical properties are presented. Conclusions important for the design of power stations fitted to the atmospheric conditions in Poland are given. LabVIEW 2021 was used for computer modeling.

Breast cancer is associated with high mortality and morbidity rates. As about 20–30% of patients exhibiting ER-positive phenotype are resistant to hormonal treatment with the standard drug tamoxifen, finding new therapies is a necessity. Postbiotics, metabolites, and macromolecules isolated from probiotic bacteria cultures have been proven to have sufficient bioactivity to exert prohealth and anticancer effects, making them viable adjunctive agents for the treatment of various neoplasms, including breast cancer. In the current study, postbiotics derived from L. plantarum and L. rhamnosus cultures were assessed on an in vitro breast cancer model as potential adjunctive agents to therapy utilizing tamoxifen and a candidate aziridine–hydrazide hydrazone derivative drug. Cell viability and cell death processes, including apoptosis, were analyzed for neoplastic MCF-7 cells treated with postbiotics and synthetic compounds. Cell cycle progression and proliferation were analyzed by PI-based flow cytometry and Ki-67 immunostaining. Postbiotics decreased viability and triggered apoptosis in MCF-7, modestly affecting the cell cycle and showing a lack of negative impact on normal cell viability. Moreover, they enhanced the cytotoxic effect of tamoxifen and the new candidate drug toward MCF-7, accelerating apoptosis and the inhibition of proliferation. This illustrates postbiotics’ potential as natural adjunctive agents supporting anticancer therapy based on synthetic drugs.

This article proposes a modular system for prosumer installations composed of photovoltaic (PV) panels and energy storage (ES) integrated with the low voltage (LV) network through a common 4-wire AC/DC inverter. The novel idea is a control strategy for the inverter in which additional functionalities are incorporated. Apart from transmitting an active power generated by the PV source, the same inverter is used to manage energy generated by the PV and to compensate for the current unbalance, harmonics (including subharmonics and interharmonics) and reactive power of the prosumer loads. As a result of the algorithm operation, the currents flowing to the prosumer installation are sinusoidal, symmetrical and purely active, which results in voltage balancing and improving voltage waveforms at the point of common coupling (PCC). In this way, with the widespread use of this solution among prosumers, the impact of the prosumer installation on the distribution network is minimized, and power quality (PQ) disturbances such as unacceptable voltage rises, voltage unbalance and harmonics are avoided. The presented approach may be a solution to the problems network operators face nowadays due to the uncontrolled connection of PV sources. The proposed modular system is also beneficial for the prosumer as the instances of unacceptable overvoltage and, consequently, shutdowns of prosumer installations are reduced. The features of the proposed method are shown in relation to other means applied for PQ improvement in the networks with distributed generation. A principle of the control and the involving algorithm for the inverter is presented. The efficiency of the control strategy was tested in a simulation developed in the PSCAD/EMTDC program. The results of simulations are presented, and the proposed solution is concluded.

Every year, ulcerative dermal necrosis (UDN) affects salmonids that spend most of their lives in the sea during their migration to the rivers of northern Poland to spawn. The clinical form of the disease manifests itself in ulcerative skin lesions, which lead to significant weakening of the fish and, in most cases, result in their death. This study was carried out on samples taken from sea trout in the Słupia River in northern Poland. In order to identify the pathogen, experiments on the transmission of the disease were carried out, and additional histopathological, microbiological and electron microscopic examinations were performed. As a result of these studies, it was possible to experimentally transfer the disease from sick to healthy fish. The results indicate a complex etiology of the disease (lack of a clearly defined pathogen), in which the change in the environment from salty to freshwater triggers the related changes in skin physiology, which are the main causes of increased susceptibility to the development of the disease.

Regaining greater independence in performing daily activities constitutes a priority for people with tetraplegia following spinal cord injury (SCI). The highest expectations are connected with the improvement of hand function. Therefore, it is so important for the clinician to identify reliable and commonly applicable prognostic factors for functional improvement. The aim of this study was to conduct an analysis to assess the impact of initial functional factors on the clinical improvement in patients during early neurological rehabilitation (ENR). This study assessed 38 patients with complete SCI aged 17–78 who underwent ENR in 2012–2022. The analysis included the motor score from the AIS (MS), the Barthel Index (BI) and the SCIM scale values at the beginning of the ENR program and after its completion. During ENR, patients achieved a statistically significant improvement in MS, BI and SCIM. The initial MS and the level of neurological injury constituted the predictors of functional improvement during ENR. Significant statistical relationships were observed primarily in the correlations between the initial MS and BI, and the increase in the analyzed functional scales of SCI patients. Higher initial MS may increase the chances of a greater and faster functional improvement during ENR.

Background: Gliomas are the most malignant tumors of the central nervous system. One of the factors in their high drug resistance is avoiding programmed death (PCD) induction. This is related to the overexpression of intracellular survival pathways: PI3K-Akt/PKB-mTOR and Ras-Raf-MEK-ERK. Apoptosis and autophagy are co-existing processes due to the interactions between Bcl-2 and beclin-1 proteins. Their complex may be a molecular “toggle-switch” between PCD types. The aim of this research was to investigate the role of Bcl-2:beclin-1 complex in glioma cell elimination through the combined action of LY294002 and sorafenib. Methods: Drug cytotoxicity was estimated with an MTT test. The type of cell death was evaluated using variant microscopy techniques (fluorochrome staining, immunocytochemistry, and transmission electron microscopy), as well as the Bcl-2:beclin-1 complex formation and protein localization. Molecular analysis of PCD indicators was conducted through immunoblotting, immunoprecipitation, and ELISA testing. SiRNA was used to block Bcl-2 and beclin-1 expression. Results: The results showed the inhibitors used in simultaneous application resulted in Bcl-2:beclin-1 complex formation and apoptosis becoming dominant. This was accompanied by changes in the location of the tested proteins. Conclusions: “Switching” between apoptosis and autophagy using PI3K and Raf inhibitors with Bcl-2:beclin-1 complex formation opens new therapeutic perspectives against gliomas.

Liverworts contain a large number of biologically active compounds that are synthesised and stored in their oil bodies. However, knowledge about the chemical composition of individual species is still incomplete. The subject of the study was Calypogeia integristipula, a species representing leafy liverworts. Plant material for chemotaxonomic studies was collected from various locations in Poland. The chemical composition was determined in 74 samples collected from the natural environment in 2021 and 2022 in three growing seasons: spring, summer and autumn, and for comparison with samples originating from in vitro culture. The plants were classified as Calypogeia integristipula on the basis of morphological characteristics, oil bodies, and DNA markers. The volatile organic compounds (VOCs) from the biological material were extracted by headspace solid phase microextraction (HS-SPME). The samples were then analysed by gas chromatography–mass spectrometry (GC-MS). A total of 79 compounds were detected, of which 44 compounds were identified. The remaining compounds were described using the MS fragmentation spectrum. Cyclical changes in the composition of compounds associated with the growing season of Calypogeia integristipula were observed. Moreover, samples from in vitro culture and samples taken from the natural environment were shown to differ in the composition of chemical compounds. In terms of quantity, among the volatile compounds, compounds belonging to the sesquiterpene group (46.54–71.19%) and sesqiuterpenoid (8.12–22.11%) dominate. A smaller number of compounds belong to aromatic compounds (2.30–10.96%), monoterpenes (0.01–0.07%), monoterpenoids (0.02–0.33%), and aliphatic hydrocarbons (1.11–6.12%). The dominant compounds in the analysed liverworts were: anastreptene (15.27–31.14%); bicyclogermacrene (6.99–18.09%), 4,5,9,10-dehydro-isolongifolene (2.00–8.72%), palustrol (4.95–9.94%), spathulenol (0.44–5.11%).

The trend to replace traditional fossil fuel vehicles is becoming increasingly apparent. The replacement concerns the use of pure biofuels or in blends with traditional fuels, the use of hydrogen as an alternative fuel and, above all, the introduction of electric propulsion. The introduction of new types of vehicle propulsion affects the demand for specific fuels, the needs for new infrastructure, or the nature of the emissions to the environment generated by fuel production and vehicle operation. The article presents a mathematical model using the difference of two logistic functions, the first of which describes the development of the production of a specific type of vehicle, and the second, the withdrawal of this type of vehicle from traffic after its use. The model makes it possible to forecast both the number of vehicles of each generation as a function of time, as well as changes in energy demand from various sources and changes in exhaust emissions. The results of the numerical simulation show replacing classic vehicles with alternative vehicles increases the total energy demand if the generation of the next generation occurs earlier than the decay of the previous generation of vehicles and may decrease in the case of overlapping or delays in the creation of new vehicles compared to the course of the decay function of the previous generation. For electric vehicles, carbon dioxide emissions are largely dependent on the emissions from electricity generation. The proposed model can be used to forecast technology development variants, as well as analyze the current situation based on the approximation of real data from Vehicle Registration Offices.

Facioscapulohumeral dystrophy (FSHD) is a muscle disease caused by inappropriate expression of the double homeobox 4 (DUX4) gene in skeletal muscle, and its downstream activation of pro-apoptotic transcriptional programs. Inhibitors of DUX4 expression have the potential to treat FSHD. Apabetalone is a clinical-stage bromodomain and extra-terminal (BET) inhibitor, selective for the second bromodomain on BET proteins. Using primary human skeletal muscle cells from FSHD type 1 patients, we evaluated apabetalone for its ability to counter DUX4′s deleterious effects and compared it with the pan-BET inhibitor JQ1, and the p38 MAPK inhibitor—and DUX4 transcriptional repressor—losmapimod. We applied RNA-sequencing and bioinformatic analysis to detect treatment-associated impacts on the transcriptome of these cells. Apabetalone inhibited the expression of DUX4 downstream markers, reversing hallmarks of FSHD gene expression in differentiated muscle cells. JQ1, but not apabetalone, was found to induce apoptosis. While both BET inhibitors modestly impacted differentiation marker expression, they did not affect myotube fusion. Losmapimod also reduced expression of DUX4 target genes but differed in its impact on FSHD-associated pathways. These findings demonstrate that apabetalone inhibits DUX4 target gene expression and reverses transcriptional programs that contribute to FSHD pathology, making this drug a promising candidate therapeutic for FSHD.

In this work, we review some physical methods of macroscopic experiments, which have been recently argued to be promising for the acquisition of valuable characteristics of biomolecular structures and interactions. The methods we focused on are electron paramagnetic resonance spectroscopy, Raman spectroscopy, and differential scanning calorimetry. They were chosen since it can be shown that they are able to provide a mutually complementary picture of the composition of cellular envelopes (with special attention paid to mycobacteria), transitions between their molecular patterning, and the response to biologically active substances (reactive oxygen species and their antagonists—antioxidants—as considered in our case study).

Immunoglobulin A (IgA) vasculitis is the most common systemic vasculitis in the pediatric population. We present the case of a patient with IgA vasculitis with nephritis who developed cytomegalovirus (CMV) infection followed by Mycobacterium tuberculosis infection. In the literature, there are a few cases of IgA nephropathy accompanied by reactivation of CMV or tuberculosis. To the best of our knowledge, this is the first reported case of IgA vasculitis complicated by both CMV reactivation and tuberculosis. It is important to detect infections in patients with IgA vasculitis because they can induce and exacerbate the symptoms of the disease. Effective antimicrobial treatment facilitates the management of proteinuria and slows down the decline of renal function. Immunosuppressive therapy is a risk factor for reactivation of latent infections and makes patients more susceptible to its generalized and complicated course. This can be prevented by actively screening for hidden sites of infection.

Epigenetic mechanisms are implicated in transcriptional programs driving chronic kidney disease (CKD). Apabetalone is an orally available inhibitor of bromodomain and extraterminal (BET) proteins, which are epigenetic readers that modulate gene expression. In the phase 3 BETonMACE trial, apabetalone reduced risk of major adverse cardiac events (MACE) by 50% in the CKD subpopulation, indicating favorable effects along the kidney–heart axis. Activation of human renal mesangial cells (HRMCs) to a contractile phenotype that overproduces extracellular matrix (ECM) and inflammatory cytokines, and promotes calcification, frequently accompanies CKD to drive pathology. Here, we show apabetalone downregulated HRMC activation with TGF-β1 stimulation by suppressing TGF-β1-induced α-smooth muscle actin (α-SMA) expression, α-SMA assembly into stress fibers, enhanced contraction, collagen overproduction, and expression of key drivers of fibrosis, inflammation, or calcification including thrombospondin, fibronectin, periostin, SPARC, interleukin 6, and alkaline phosphatase. Lipopolysaccharide-stimulated expression of inflammatory genes IL6, IL1B, and PTGS2 was also suppressed. Transcriptomics confirmed apabetalone affected gene sets of ECM remodeling and integrins. Clinical translation of in vitro results was indicated in CKD patients where a single dose of apabetalone reduced plasma levels of key pro-fibrotic and inflammatory markers, and indicated inhibition of TGF-β1 signaling. While plasma proteins cannot be traced to the kidney alone, anti-fibrotic and anti-inflammatory effects of apabetalone identified in this study are consistent with the observed decrease in cardiovascular risk in CKD patients.

This paper concerns the mitigation of voltage disturbances deteriorating power quality and disrupting the operation of LV distribution grids due to the high penetration of PV energy sources in prosumer installations. A novel control strategy for 3-phase 4-wire PV inverters is proposed, which ensures the transmission of PV active power and simultaneous compensation of load unbalance and reactive power, making the prosumer installation balanced and purely active. It results in the balance of phase voltages and the mitigation of their variability. Unlike other methods used for voltage regulation in LV grids, the proposed solution contributes to the reduction in losses, is simple, and does not require additional costs. In the paper, a control algorithm for the PV inverter is described. Its effectiveness was tested by simulation using a model of the real LV distribution grid developed in the PSCAD/EMTDC program. The results of the simulations are presented and evaluated.

The solution enthalpy of 15-crown-5 and 18-crown-6 ethers in the mixture of formamide (F) and water (W) was measured at four temperatures: 293.15 K, 298.15 K, 303.15 K, 308.15 K. The standard molar enthalpy of solution, ΔsolHo, depends on the size of cyclic ethers molecules and the temperature. With increasing temperature, the values of ΔsolHo become less negative. The values of the standard partial molar heat capacity Cp,2o of cyclic ethers at 298.15 K have been calculated. The Cp,2o=f(xW) curve shape indicates the hydrophobic hydration process of cyclic ethers in the range of a high-water content in the mixture with formamide. The enthalpic effect of preferential solvation of cyclic ethers was calculated and the effect of temperature on the preferential solvation process was discussed. The process of complex formation between 18C6 molecules and formamide molecules is observed. The cyclic ethers molecules are preferentially solvated by formamide molecules. The mole fraction of formamide in the solvation sphere of cyclic ethers has been calculated.

Pentobarbital is a drug of choice to limit motion in children during paediatric procedural sedations (PPSs). However, despite the rectal route being preferred for infants and children, no pentobarbital suppositories are marketed, and therefore they must be prepared by compounding pharmacies. In this study, two suppository formulations of 30, 40, 50, and 60 mg of pentobarbital sodium were developed using hard-fat Witepsol® W25 either alone (formulation F1) or with oleic acid (formulation F2). The two formulations were subjected to the following tests described in the European Pharmacopoeia: uniformity of dosage units, softening time, resistance to rupture, and disintegration time. The stability of both formulations was also investigated for 41 weeks of storage at 5 ± 3 °C using a stability-indicating liquid chromatography method to quantify pentobarbital sodium and research breakdown product (BP). Although both formulae were compliant to uniformity of dosage, the results were in favour of a faster disintegration of F2 compared to F1 (−63%). On the other hand, F1 was found to be stable after 41 weeks of storage unlike F2 for which several new peaks were detected during the chromatographic analysis, suggesting a shorter stability of only 28 weeks. Both formulae still need to be clinically investigated to confirm their safety and efficiency for PPS.

This article describes the problems associated with distributed electrical power generation and the most frequent interruptions occurring in power grids. The most common methods of improving the quality of the power supply were analyzed and the possibilities offered by energy storages in this respect were considered. The operating parameters of an exemplary PV system connected to the power grid were analyzed. For this purpose, the model implemented in the Matlab/Simulink environment was used. Based on the conducted analysis and a review of the literature, conclusions were drawn and solutions were presented which could improve the quality and the reliability of power supply. The simulations conducted focused on the co-operation of individual photovoltaic, micro-installations, with rated powers of 12.2, 19.825, and 39.65 Kw in the power grid, which also corresponds to the co-operation of several, smaller micro-installations with low density.

The growth in the number of cars and the increasing demand for fuels require scientific work to develop alternative fuels. The energy crisis, which is becoming more and more evident, is not unimportant. The manuscript presents an analysis of the possibility of using agricultural biofuels to power a diesel engine. The analysis was carried out in relation to the operation of the engine on conventional fuels. The D-241 engine under investigation is mainly used for agricultural tractors. During the tests carried out, the load characteristics of the diesel engine under investigation were determined as a function of fuel type and speed. The concentrations of CO, HC and NOx were analysed. Laboratory tests of the engine were carried out with a wide range of external loads to evaluate measurement errors for the measurement method. Experiments with the engine under investigation have shown that the hourly and specific biofuel consumption of diesel engines increases by an average of 11–16%. CO and NOx concentrations were found to be lower with increasing load compared to conventional diesel engines, while NOx concentrations are slightly increased. In all cases investigated, a decrease in exhaust development was observed.

Mycoplasma bovis (M. bovis) is an important pathogen affecting cattle, causing various diseases including pneumonia which mainly occurring in calves. Control of M. bovis infections is difficult due to the lack of commercial vaccines in most parts of the world and increasing trends of antimicrobial resistance in field isolates of the pathogen; therefore, it seems reasonable to look for new solutions for the prevention of the infection. Pegbovigrastim is a pegylated form of naturally occurring circulating cytokine in cattle that affects bovine leukocytes and some cell functions. Most studies on pegbovigrastim have focused on reducing the occurrence of mastitis and other diseases occurring during the periparturient period in cows, while this study attempts to use pegbovigrastim in the prevention of respiratory diseases in calves, which are largely caused by M. bovis. Based on previous observations on the immunostimulatory properties of pegbovigrastim in cattle, for the first time, the effect of its injection on the number and phagocytic and oxidative burst activities of peripheral blood granulocytes and monocytes in calves experimentally infected with M. bovis was investigated. Pegbovigrastim administration in the calves significantly stimulated an increase in peripheral blood granulocyte and monocyte counts and phagocytic activity of the cells, especially granulocytes, which was also generally expressed in the course of M. bovis infection. In response to pegbovigrastim administration, a general increase in the oxygen burst activity of the cells was observed. This effect was also shown despite ongoing infection with M. bovis which, taken together, may indicate a beneficial effect of pegbovigrastim injection on the immunity of the affected animals.

During the initial months of calves’ lives, the young animals are exposed to bacterial and viral infections, and during this period, crucial physiological changes take place in their organisms. Offering calves feed additives that will have a beneficial influence on their organisms and improve their growth while reducing the morbidity rate is the optimal task of feeding. This is the first study to investigate the effect of experimental supplementation for calves with the combination of two feed additives—one containing Lentinula edodes enriched with selenium (Se), and the second containing pancreatic-like enzymes, fat-coated organic acids, sodium butyrate, and silicon dioxide nanoparticles—on the serum Se concentration, selected immune parameters, and the average daily gains in the calves. During the study, the serum Se concentration was examined by means of inductively coupled plasma mass spectrometry, and the immunoglobulin and cytokine concentrations with ELISA assays. The white blood cell (WBC) count with leukocyte differentiation was examined with the use of a hematological analyzer, and the percentages of subpopulations of T lymphocytes and monocytes, phagocytic activity, and oxidative burst of monocytes and granulocytes with the use of a flow cytometer. The average daily gains of the calves were also evaluated. In summary, the supplementation of the experimental calves with the combination of two feed additives resulted in significantly higher serum Se concentrations, and the immune systems of the calves were not suppressed while the examined feed additives were being delivered. Although not statistically significant, some positive effects on the calves were seen: a tendency towards the improvement of some of the immune parameters evaluated, and a tendency for higher average daily gains in the calves.

Infectious pancreatic necrosis virus (IPNV) often occurs in an aquatic environment in co-infection with other viruses. In this study, we wanted to investigate the effect of this virus on the course of co-infection with other viruses in rainbow trout. For co-infection we used viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV) and salmonid alphavirus (SAV) field strains and infected rainbow trout divided into eight groups; I; IPNV, II; IHNV, III; VHSV, I; SAV, V; IPNV+IHNV, VI; IPNV+VHSV, VII; IPNV+SAV, and the control group. We assessed apoptosis in white blood cells and used a real time RT-PCR to analyze RNA obtained from the internal organs of the fish. During single infection and co-infection the level of expression of immune genes such as interferon and toll-like receptor 3 (TLR-3) was assessed. The highest mortality during the experiment was observed in group III infected by VHSV. The average percentage of apoptotic cells was higher in groups without co-infection, especially in groups II and III. Interferon expression was higher in singly infected groups, the highest being in the heart in group III, while expression of the TLR-3 gene was generally raised in all tested organs in all groups. We found that co-infection with IPNV had a positive impact on the course of infection with the viruses listed because it lowered mortality, reduced apoptosis in co-infected cells, and positively affected fish health.

This article focuses on the comparison of four popular techniques for the extraction of volatile organic compounds (VOCs) from liverworts of the Calypogeia azurea species. Since extraction is the most important step in the sample analysis of ingredients present in botanical preparations, their strengths, and weaknesses are discussed. In order to determine the VOCs present in plants, selecting the appropriate one is a key step of the extraction technique. Extraction should ensure the isolation of all components present in the oily bodies of Calypogeia azurea without the formation of any artifacts during treatment. The best extraction method should yield the determined compounds in detectable amounts. Hydrodistillation (HD), applying Deryng apparatus and solid-liquid extraction (SLE), microwave-assisted extraction (MAE), and headspace solid-phase microextraction (HS-SPME) were used for volatile extraction. The extracts obtained were analysed by gas chromatography coupled to mass spectrometry (GC-MS) to determine the compounds.

A change in the composition of specialized metabolites is often observed in stressed plants. Phytochemicals play an important role in adapting plants to the environment, particularly overcoming stress conditions such as temperature, humidity, and light intensity. In this study, seasonal variations in the concentrations of volatile organic compounds (VOCs) were analysed in species of Calypogeia azurea. The article presents the effect of sample storage on volatile organic compounds present in Calypogeia liverwort cells and whether the collection habitats of the sample affect the content of phytochemicals. The VOCs of the species within the liverwort Calypogeia azurea were analysed by GC-MS. Compounds were isolated from plant material using the HS-SPME technique. The samples were collected over several years (2019–2021). Of the several dozen samples collected, 79 compounds were isolated, of which 47 were identified.

This paper presents the use of real-time digital simulator (RTDS) and hardware-in-the-loop (HIL) methods for the validation of an energy management system designed for real low-voltage (LV) distribution networks with a high penetration of renewable energy sources. The system is used to address voltage violations and current overloading issues and allows the network operator to maintain safe and controllable network operations. The applied control strategy and the system software were verified by means of simulations. In this paper, the next stage of system validation using the HIL method is presented. A testbed was designed and developed to test the operation of prototype controllers of the system in flexible and reproducible conditions before installing them in the network. The presented testing platform not only includes the LV network simulator with the power amplifiers needed for closed-loop setup but also additional elements of a real network to which the system is dedicated, i.e., the advanced metering infrastructure, photovoltaic source, and energy storage inverters and load devices. Furthermore, the real cellular network of the distribution network operator is used in the communication between the controllers. In addition, the article contains discussions on communication issues, including limitations related to selected protocols. Finally, examples of the experimental validation of the controller prototypes are presented.

Tacrolimus is an immunosuppressant used to treat a large variety of inflammatory or immunity-mediated ophthalmic diseases. However, there are currently no commercial industrial forms available that can provide relief to patients. Various ophthalmic formulations have been reported in the literature, but their stability has only been tested over short periods. The objective of this study was to evaluate the physicochemical stability of a preservative-free tacrolimus formulation (0.2 and 1 mg/mL) at three storage temperatures (5 °C, 25 °C and 35 °C) for up to nine months in a multidose eyedropper. Analyses performed were the following: visual inspection and chromaticity, turbidity, viscosity, size of micelles, osmolality and pH measurements, tacrolimus quantification by a stability-indicating liquid chromatography method, breakdown product research, and sterility assay. In an in-use study, tacrolimus quantification was also performed on the drops emitted from the eyedroppers. All tested parameters remained stable during the nine month period when the eyedrops were stored at 5 °C. However, during storage at 25 °C and 35 °C, several signs of chemical instability were detected. Furthermore, a leachable compound originating from a silicone part of the eyedropper was detected during the in-use assay. Overall, the 0.2 mg/mL and 1 mg/mL tacrolimus ophthalmic solutions were physicochemically stable for up to nine months when stored at 5 °C.

The transport sector is one of the main barriers to achieving the European Union’s climate protection objectives. Therefore, more and more restrictive legal regulations are being introduced, setting out permissible limits for the emission of toxic substances emitted into the atmosphere, promoted biofuels and electromobility. The manuscript presents a computer tool to model the total energy consumption and carbon dioxide emissions of vehicles with an internal combustion engine of a 2018 Toyota Camry LE. The calculation tool is designed in the OpenModelica environment. Libraries were used for this purpose to build models of vehicles in motion: VehicleInterfaces, EMOTH (E-Mobility Library of OTH Regensburg). The tool developed on the basis of actual driving test data for the selected vehicle provides quantitative models for the instantaneous value of the fuel stream, the model of the instantaneous value of the carbon dioxide emission stream as a function of speed and the torque generated by the engine. In the manuscript, the tests were conducted for selected driving cycles tests: UDDS (EPA Urban Dynamometer Driving Schedule), HWFET (Highway Fuel Economy Driving Schedule), EPA US06 (Environmental Protection Agency; Supplemental Federal Test Procedure (SFTP)), LA-92 (Los Angeles 1992 driving schedule), NEDC (New European Driving Cycle), and WLTP (Worldwide Harmonized Light-Duty Vehicle Test Procedure). Using the developed computer tool, the impact on CO2 emissions was analyzed in the context of driving tests with four types of fuels: petrol 95, ethanol, methanol, DME (dimethyl ether), CNG (compressed natural gas), and LPG (liquefied petroleum gas).

Organic agriculture is considered one of the elements of sustainable food production and consumption, mainly due to its limited impact on the natural environment. At the same time, the quality features of organically produced foods, especially sensory attributes and health promoting values, are important factors determining consumers’ interest, and therefore play a key role in the organic sector’s development. The aim of this study was to investigate the sensory characteristics and concentrations of sugars and selected health-promoting bioactive compounds of organic courgette compared to conventionally grown courgette. In addition, untargeted metabolomic analysis of the courgette fruits was performed. The results of this study did not show a significant effect of the horticultural system (organic vs. conventional) on the concentrations of vitamin C, carotenoids, and chlorophylls in the courgette fruits. However, the fruits from the organic systems were significantly richer in sugars when compared to the conventionally cultivated ones (p = 0.038). Moreover, the organic fruits fertilized with manure contained significantly higher amounts of polyphenols, including gallic acid (p = 0.016), chlorogenic acid (p = 0.012), ferulic acid (p = 0.019), and quercetin-3-O-rutinoside (p = 0.020) compared to the conventional fruits. The untargeted analysis detected features significantly differentiating courgette fruits depending on the cultivar and horticultural system. Some significant differences in sensory values were also identified between fruits representing the two cultivars and coming from the horticultural systems compared in the study. Conventional courgettes were characterized by the most intensive peel color and aquosity, but at the same time were the least hard and firm compared to the fruits from the two organic systems. There was also a trend towards higher overall quality of the organically grown fruits. The presented study shows that the organic and conventional courgette fruits differ in a number of quality features which can influence consumers’ health and purchasing choices.

Alaria alata flukes are cosmopolitan parasites. In Europe, the definitive hosts are red foxes (Vulpes vulpes), wolves (Canis lupus), and raccoon dogs (Nyctereutes procyonoides), as well as animals that belong to the Felidae family. Intermediate hosts, such as snails and frogs, are the sources of infection for definitive hosts. The developmental stages of A. alata mesocercariae may occur in paratenic hosts, including many species of mammals, birds, and reptiles, as well as in wild boars (Sus scrofa), which are important from the zoonotic point of view. Because there are no regulations concerning the detection of A. alata in meat, this fluke is usually detected during official obligatory Trichinella spp. inspections. However, a method dedicated to A. alata detection was developed. The growing popularity of game and organic meat has led to an increased risk of food-associated parasitic infections, including alariosis, which is caused by the mesocercarial stage of A. alata. The aim of this article is to highlight the problem of A. alata as an emerging parasite, especially in the terms of the increasing market for game and organic meats that have been processed with traditional methods, often without proper heat treatment.

Modern consumer installations can be equipped with renewable power sources (RESs) and stationary energy storage systems (ESSs). In addition, electric vehicles (EVs) are expected to become part of such installations in the not-too-distant future. The paper presents the control strategy that allows for efficient energy management and the option of EV “fast-home” charging. The novelty of this approach includes the use of the “time-dependent energy storage” (EV battery) together with ESS and PV sources with the focus on prosumer benefits. All goals can be achieved without the need for extensive expenses in the home electric infrastructure. To enable the synergy effect, it was necessary to develop a controller algorithm that uses the operating status of the prosumer infrastructure (current power generation and consumption), the state of charge of both the stationary storage and the EV battery, and the possibility to control the EV drive inverter during the parking state. The paper presents a developed simulator built in the PSCAD environment and the simulation results.

The article presents an original simulation model of a wind farm (WF) consisting of 30 wind turbine-generator units connected to the electrical power system (EPS) through power converters. The model is dedicated to the evaluation of the WF capabilities to participate in frequency and voltage regulation services in the power system. A system that allows for frequency and voltage control is proposed and implemented in the presented model. The system includes primary frequency regulation with synthetic inertia and secondary regulation available on request from the system operator. The concept of a reference power generation unit was introduced, according to which only one wind generator unit was modeled in detail, and the other units were replaced with simple current sources. Such a solution allowed for the reduction of size and complexity of the model as well as shortened the simulation time. Simulation tests were conducted in the PSCAD/EMTDC environment for an electrical power system composed of the wind farm, a synchronous generator, and a dummy load. The performance of the wind farm control system was analyzed in different operation conditions, and the control capabilities of the farm were assessed. Selected simulation results are presented and discussed in the paper. They illustrate the regulatory properties of the WF and confirm the correctness of the developed model.

In recent years, policymakers of urban agglomerations in various regions of the world have been striving to reduce environmental pollution from harmful exhaust and noise emissions. Restrictions on conventional vehicles entering the inner city are being introduced and the introduction of low-emission measures, including electric ones, is being promoted. This paper presents a method for scenario analysis applied to study the reduction of exhaust emissions by introducing electric vehicles in a selected city. The original scenario analyses relating to real problems faced by contemporary metropolitan areas are based on the VISUM tool (PTV Headquarters for Europe: PTV Planung Transport Verkehr AG, 76131 Karlsruhe, Germany). For the case study, the transport model of the city of Bielsko-Biala (Poland) was used to conduct experiments with different forms of participation of electric vehicles on the one hand and traffic restrictions for high emission vehicles on the other hand. Scenario analyses were conducted for various constraint options including inbound, outbound, and through traffic. Travel time for specific transport relations and the volume of harmful emissions were used as criteria for evaluating scenarios of limited accessibility to city zones for selected types of vehicles. The comparative analyses carried out showed that the introduction of electric vehicles in the inner city resulted in a significant reduction in the emission of harmful exhaust compounds and, consequently, in an increase in the area of clean air in the city. The case study and its results provide some valuable insights and may guide decision-makers in their actions to introduce both driving ban restrictions for high-emission vehicles and incentives for the use of electric vehicles for city residents.

This study aimed to indicate the influence of infection caused by genotype II African swine fever virus (ASFV)–isolate Pol18_28298_O111, currently circulating in Poland, on blood counts, biochemical parameters, as well as inflammatory and immune responses. Blood and sera collected from 21 domestic pigs infected intranasally with different doses of virulent ASFV were analysed. The infection led to variable changes in blood counts depending on the stage of the disease with a tendency towards leukopenia and thrombocytopenia. The elevated C-reactive protein (CRP) concentrations and microscopic lesions in organs confirmed the development of the inflammation process, which also resulted in an increased level of biochemical markers such as: Aspartate transaminase (AST), creatine kinase (CK), creatinine, and urea. Antibodies could be detected from 9 to 18 days post infection (dpi). Two survivors presented the highest titer of antibodies (>5 log10/mL) with a simultaneous increase in the lymphocyte T (CD3+) percentage–revealed by flow cytometry. Results confirmed a progressive inflammatory process occurring during the ASFV infection, which may lead to multiple organs failure and death of the majority of affected animals.

Aneura pinguis (L.) Dumort. is a representative of the simple thalloid liverworts, one of the three main types of liverwort gametophytes. According to classical taxonomy, A. pinguis represents one morphologically variable species; however, genetic data reveal that this species is a complex consisting of 10 cryptic species (named by letters from A to J), of which four are further subdivided into two or three evolutionary lineages. The objective of this work was to develop an efficient method for the characterisation of plant material using marker compounds. The volatile chemical constituents of cryptic species within the liverwort A. pinguis were analysed by GC-MS. The compounds were isolated from plant material using the HS-SPME technique. Of the 66 compounds examined, 40 were identified. Of these 40 compounds, nine were selected for use as marker compounds of individual cryptic species of A. pinguis. A guide was then developed that clarified how these markers could be used for the rapid identification of the genetic lineages of A. pinguis. Multivariate statistical analyses (principal component and cluster analysis) revealed that the chemical compounds in A. pinguis made it possible to distinguish individual cryptic species (including genetic lineages), with the exception of cryptic species G and H. The classification of samples based on the volatile compounds by cluster analysis reflected phylogenetic relationships between cryptic species and genetic lineages of A. pinguis revealed based on molecular data.

The paper includes the analysis of the operation of low-voltage prosumer installation consisting of receivers and electricity sources and equipped with a 3-phase energy storage system. The aim of the storage application is the management of active power within the installation to decrease the total power exchanged with the supplying network and thus reduce energy costs borne by the prosumer. A solution for the effective implementation of the storage system is presented. Apart from the active power management performed according to the prosumer’s needs, the storage inverter provides the ancillary service of voltage regulation in the network according to the requirements of the network operator. A control strategy involving algorithms for voltage regulation without prejudice to the prosumer’s interest is described in the paper. Reactive power is used first as a control signal and if the required voltage effect cannot be reached, then the active power in the controlled phase is additionally changed and the Energy Storage System (ESS) loading is redistributed in phases in such a way that the total active power set by the prosumer program remains unchanged. The efficiency of the control strategy was tested by means of a simulation model in the PSCAD/EMTDC program. The results of the simulations are presented.

Modern cuisine served at top-end restaurants attempts to attract customers, who increasingly demand new flavor, pleasure and fun. The materials were six dishes prepared using lemon or tomatoes and made in the traditional (classical), molecular and Note by Note (NbN) versions. The study explores sensory characteristics, consumer liking of key attributes, their declared sensations and emotions, as well as consumers’ facial expressions responding to the dishes. These objectives were investigated by descriptive quantitative analysis and consumer tests. Tests included a 9-point hedonic scale for degree of liking a dish, Check-All-That-Apply (CATA) for declared sensations and FaceReader for facial expressions. The influence of factors associated with consumer attitudes toward new food and willingness to try the dishes in the future were also determined. It was stated that the product profiles represent different sensory characteristics due to the technology of food production and the ingredients used. The food neophobia and consumer innovativeness had a significant (p ≤ 0.05) effect on liking. The odor-, flavor-, texture- and overall-liking of the NbN dishes were lower than that of traditional versions but did not vary from scores for molecular samples. The expected liking of NbN dishes was higher than experienced-liking. Traditional and modern products differed in CATA terms. Classical dishes were perceived by consumers as more tasty, traditional and typical while modern cuisine dishes were perceived as more surprising, intriguing, innovative and trendy. Mimic expressions assessment by FaceReader showed similar trends in some emotions in both classical dishes and separate temporal patterns in modern products.

The paper concerns the analysis of harmful emissions during the combustion process in households. The subject of the analysis is a low emission heating device with an output of 50 kW for burning biomass of forest origin (low-quality hardwoods or softwoods). The proposed boiler is automatically fed from the connected container by means of a screw conveyor. In this way, the optimum amount of fuel is supplied for maximum heat output (adjustment of the ratio of primary air to fuel). The proposed biomass heating system is equipped with a primary and secondary air supply system and exhaust gas sensors. This ensures optimal regulation of the air mixture and efficient and clean combustion. Proper control of the combustion process, control of the air supply by means of a lambda sensor and power control of the system ensure a low-emission combustion process. The system precisely adjusts to the heat demand. This results in highly efficient heating technology with low operating costs. In the presented work, the emission of exhaust gases from the proposed heating device during the combustion of woodchips and beech–oak pellets were measured. It is demonstrated that the proposed design of the boiler equipped with intelligent control significantly reduces emissions when the biomass solid fuels are used, e.g., CO emissions from beech and oak chips and pellets in the low-emission boiler—18 extract pipes shows the value

Myopia is an ophthalmic condition affecting more than 1/5th of the world population, especially children. Low-dose atropine eyedrops have been shown to limit myopia evolution during treatment. However, there are currently no commercial industrial forms available and there is little data published concerning the stability of medications prepared by compounding pharmacies. The objective of this study was to evaluate the stability of two 0.1 mg/mL atropine formulations (with and without antimicrobiobial preservatives) for 6 months in two different low-density polyethylene (LDPE) multidose eyedroppers. Analyses used were the following: visual inspection, turbidity, chromaticity measurements, osmolality and pH measurements, atropine quantification by a stability-indicating liquid chromatography method, breakdown product research, and sterility assay. In an in-use study, atropine quantification was also performed on the drops emitted from the multidose eyedroppers. All tested parameters remained stable during the 6 months period, with atropine concentrations above 94.7% of initial concentration. A breakdown product (tropic acid) did increase slowly over time but remained well below usually admitted concentrations. Atropine concentrations remained stable during the in-use study. Both formulations of 0.1 mg/mL of atropine (with and without antimicrobial preservative) were proved to be physicochemically stable for 6 months at 25 °C when stored in LDPE bottles, with an identical microbial shelf-life.

Fungal keratitis is a sight-threatening disease for which amphotericin B eye drops is one of the front-line treatments. Unfortunately, there are currently no commercial forms available, and there is little data concerning the long-term stability of compounded formulations based on intravenous dosages forms. New formulations of amphotericin B ophthalmic solutions solubilised with γ-cyclodextrins have shown promising in-vitro results, but stability data is also lacking. The objective of this study was therefore to investigate the stability of a formulation of ready-to-use amphotericin B solubilised in 2-hydroxypropyl-γ-cyclodextrins (AB-HP-γ-CD), for 350 days. An amphotericin B deoxycholate (ABDC) formulation was used as a comparator. Analyses used were the following: visual inspection, turbidity, osmolality and pH measurements, amphotericin B quantification by a stability-indicating liquid chromatography method, breakdown product research, and sterility assay. AB-HP-γ-CD formulation showed signs of chemical instability (loss of amphotericin B) after 28 and 56 days at 25 °C and 5 °C. Adding an antioxidant (ascorbic acid) to the formulation did not improve stability. ABDC formulation showed signs of physical instability (increased turbidy and amphotericin B precipitation) after 28 days and 168 days at 25 °C and 5 °C. As such, AB-HP-γ-CD formulation does not provide long-term stability for ophthalmic amphotericin B solutions.

Introduced by the Civil Code, the rules of road safety are introducing continuously increasingly strict requirements on motor vehicles. These requirements relate to various aspects of the technical condition of vehicles, both those that determine traffic safety and those that affect the vehicle’s environmental impact. The law requires regular diagnosis of the technical condition of vehicles in service. Diagnostic tests conducted in the form of road tests or the tests performed in workshop conditions allow to determine the symptoms of dysfunctions of the tested vehicle, not always clearly defining the causes and location of damage. The purpose of the work is the design the simulation of a station for of vehicle dynamics tests up to 3.5 t using simulation programs OpenModelica and SciLab. A simulation of the work of the stand for testing the dynamics of vehicles in the form of a chassis dynamometer was achieved. The program enables the simulation of tests: NEDC (New European Drive Cycle), WLTP (Worldwide Harmonized Light Vehicle Test Procedure), CADCM150 (joint Artemis driving cycle—Motorway at vMax = 150 kph), CADCU (Common Artemis Driving Cycle—Urban), FTP75 EPA (Federal Test Procedure, Environmental Protection Agency). The simulator (for any assumed type of vehicle) can be used in two modes: 1. Introduction of the presumed cause—Generates the expected results in the functioning of the vehicle. This function can be used to create a cause–effect relational database. 2. Analysis of data from the actual diagnostic system suggesting the causes of the observed (measured) errors in the functioning of the system. The simulator can be used both to design and implement the technological development of intelligent diagnostic systems, and to support the creation of application software for a workshop diagnostic system. Introducing the simulator into practice will also enable the improvement of road safety management.

This paper deals with the application of an electric vehicle (EV) motor inverter and its batteries as an energy storage device supporting the operation of home electrical installation. This additional functionality of EV is called a Vehicle-to-Home (V2H) service. Two kind of services are considered: a peak shaving and an emergency power supply. The simulation model developed in the PSCAD program is presented. It allows for the examination of the EV battery control and operation during EV driving and parking. Additionally, it allows an evaluation of the availability of home installation for the V2H service. Control algorithms enabling the implementation of discussed work options are presented. Results of simulations are presented illustrating the EV control and operation in different operational modes.

Managing of wastes rich in lignocellulose creates the opportunity to produce biofuels that are in full compliance with the principles of sustainable development. Biomass, as a suitable base for the production of biofuels, does not have to be standardized, and its only important feature is the appropriate content of lignocellulose, which assures great freedom in the selection of input. Biobutanol, obtained from this type of biomass, can be used as fuel for internal combustion engines, including diesel engines. In the era of strict environmental protection regulations, especially concerning atmospheric air, any new fuel, apart from good energetic properties, should also show beneficial ecological effects. This study investigates the carbon dioxide emissions from biobutanol powered diesel engine by means of use of the simulation model. The parameters of a real passenger car powered by a diesel engine were used for simulation carried out accordingly to the WLTP (Worldwide Harmonized Light Vehicle Test Procedure) approval procedure as the current test for newly manufactured cars. The results obtained for biobutanol were compared with simulated exhaust emissions obtained for conventional diesel and with FAME (fatty acid methyl esters)—the most popular biofuel. Biobutanol, in spite of its higher consumption, showed lower direct carbon dioxide emissions than both: the conventional diesel and FAME. In addition, a LCA (life cycle assessment) was carried out for the fuels and vehicles in question using the SimaPro package. Therefore, the implementation of butyl alcohol as a fuel provides favorable environmental effects. This result gives arguments towards biofuel production management indicating that implementation of biobutanol production technology mitigates carbon dioxide emission, as well as promotes lignocellulosic resources rather than edible parts of the plants.

Despite the widespread use of sonication for individualization of nanomaterials, its destructive nature is rarely acknowledged. In this study, we demonstrated how exposure of the material to a hostile sound wave environment can be limited by the application of another preprocessing step. Single-walled carbon nanotubes (CNTs) were initially ground in a household coffee grinder, which enabled facile deagglomeration thereof. Such a simple approach enabled us to obtain high-quality CNT dispersion at reduced sonication time. Most importantly, electrical conductivity of free-standing films prepared from these dispersion was improved almost fourfold as compared with unground material eventually reaching 1067 ± 34 S/cm. This work presents a new approach as to how electrical properties of nanocarbon ensembles may be enhanced without the application of doping agents, the presence of which is often ephemeral.

The aim of the study was to evaluate the effect of heat treatment salmon quality using the sous-vide method (57 °C, 20 min and 63 °C, 80 min) in comparison with traditional methods (steam cooking, roasting). The yield of process and sensory quality (sensory profile, consumer liking) and the color and texture of salmon was measured. Salmon processed with the sous-vide method was characterized by a statistically significantly (p ≤ 0.05) higher yield and water content than the samples prepared by steaming and roasting. Statistically higher (p ≤ 0.05) consumer preference for salmon prepared using higher parameters of the sous-vide process (63 °C, 80 min) compared with low parameters (57 °C, 20 min) was stated. Parameters of the sous-vide processing (57 °C, 20 min) have a negative effect on salmon quality. It was observed that as the temperature and time of the process increased, the intensity of the flavor and odor attributes of cooked fish also increased, while the intensity of raw fish, juiciness, tenderness, and softness decreased. The color of salmon also changed. Based on the results, we recommend the following parameters of process in preparing salmon using the sous-vide method: 63 °C for 80 min.

Intensified action aimed at reducing CO2 emissions and striving for energy self-sufficiency of both business entities and individual consumers are forcing the sustainable development of environmentally friendly and renewable energy sources. The development of an appropriate class of equipment and production technology is not without significance in this process. On the basis of a proven design for a combustion burner for ecological fuels, a new biofuel burner, also dedicated to prosumers’ energetics, was built. The aim of the study was to determine the effect of the type of biofuel on a burner’s output parameters, especially gaseous emissions, during the combustion of four types of fuels, including three types of biomass. The combustion temperature was measured for lignite, wood pellets, straw pellets, and sunflower pellets. An analysis of exhaust gas composition was performed for lignite and wood pellets. The results of exhaust emissions and combustion temperatures were compared with the burners currently in use. The use of a new burner might contribute to cleaner combustion and reducing the emissions of some gaseous components.

The power sector is currently experiencing a dynamic transformation, resulting not only from EU directives, but also from current problems, mainly related to ensuring energy security for customers. For this reason, in Poland, it was decided that the model of electricity market operation needed to be changed. A dual market has been created, and a separated segment is the capacity market. This solution operates, in addition to other power mechanisms, in many European Union countries, e.g., in France, Great Britain, and Italy. The experience of these countries indicates the positive impact of the power market on various aspects of the power sector, such as legal, economic, technical and technological, social, and infrastructural aspects, mainly due to technological neutrality and open competition between power suppliers. The creation of the structure of an energy market drastically changes the rules of energy management, which requires investigations concerning the impact of those factors on the energy sector in Poland. The aim of this work is to examine the impact of the power market on the Polish power sector, and to determine the impact of individual consequences on several predefined areas. For this purpose, a questionnaire survey was used. The purpose of a detailed analysis is to develop probable scenarios for the sustainable development of the Polish energy sector.