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Your search keyword '"Verena K. Affolter"' showing total 3 results
Start Over Author "Verena K. Affolter" Publisher mdpi ag
3 results on '"Verena K. Affolter"'

1. Nerve Fiber Immunohistochemical Panel Discriminates between Nerve Sheath and Perivascular Wall Tumors

Author

Sílvia Sisó, Paola Marco-Salazar, Paola Roccabianca, Giancarlo Avallone, Robert J. Higgins, and Verena K. Affolter

Subjects
nerve sheath tumors, NST biomarkers, immunohistochemistry, laminin, Sox-10, periaxin-1, Veterinary medicine, SF600-1100
Abstract

Benign and malignant nerve sheath tumors (NST) pose a major challenge in routine diagnostic anatomic pathology because of shared histomorphological features with other soft-tissue tumors (STT). As a result, NST are often diagnosed as STT, a broad category that encompasses various entities including perivascular wall tumors (PWT) and that represents approximately 15% of all skin tumors in dogs. Immunohistochemistry (IHC) can assist the identification of histologic subtypes of STT. This IHC pilot study applies various markers largely expressed by peripheral nerves to twelve benign and six malignant NST and determines the intratumoral protein expression of laminin, periaxin-1, Sox-10 and S-100 in the NST subtypes. Furthermore, this study assesses the usefulness of peripheral nerve markers applied to diagnostic work cases and demonstrates the relevance of laminin expression patterns, periaxin-1 and Sox-10 in assisting the differentiation of NST from other STT, in particular from PWT.

Published
2022
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2. Correction: Weich, K., et al. Pigment Intensity in Dogs Is Associated with a Copy Number Variant Upstream of KITLG. Genes 2020, 11, 75

Author

Angelica Kallenberg, Verena K. Affolter, Kalie M. Weich, Robert A. Grahn, Daniel York, Robert B. Rebhun, and Danika L. Bannasch

Subjects
0301 basic medicine, Genetics, lcsh:QH426-470, Biology, 03 medical and health sciences, lcsh:Genetics, 030104 developmental biology, 0302 clinical medicine, n/a, 030220 oncology & carcinogenesis, Upstream (networking), Copy-number variation, Gene, Genetics (clinical)
Abstract

Author(s): Bannasch, Danika L; Affolter, Verena K; York, Daniel; Rebhun, Robert B; Grahn, Robert A; Weich, Kalie M; Kallenberg, Angelica | Abstract: The authors wish to make the following corrections to this paper [...].

Published
2021

3. Pigment Intensity in Dogs is Associated with a Copy Number Variant Upstream of KITLG

Author

Kalie M. Weich, Angelica Kallenberg, Verena K. Affolter, Daniel York, Danika L. Bannasch, Robert A. Grahn, and Robert B. Rebhun

Subjects
0301 basic medicine, Coat, lcsh:QH426-470, DNA Copy Number Variations, canine, Breeding, Biology, Article, eumelanin, 03 medical and health sciences, Chromosome 15, Pigment, Dogs, 0302 clinical medicine, Genetics, Animals, Copy-number variation, Hair Color, Gene, Genotyping, coat color, Genetics (clinical), Melanins, Whole genome sequencing, Stem Cell Factor, Pigmentation, Human Genome, pheomelanin, Correction, Nova Scotia Duck-Tolling Retriever, dilution, lcsh:Genetics, 030104 developmental biology, 030220 oncology & carcinogenesis, visual_art, visual_art.visual_art_medium, Genome-Wide Association Study
Abstract

Dogs exhibit a wide variety of coat color types, and many genes have been identified that control pigment production, appearance, and distribution. Some breeds, such as the Nova Scotia Duck Tolling Retriever (NSDTR), exhibit variation in pheomelanin pigment intensity that is not explained by known genetic variants. A genome-wide association study comparing light red to dark red in the NSDTR identified a significantly associated region on canine chromosome 15 (CFA 15:23 Mb&ndash, 38 Mb). Coverage analysis of whole genome sequence data from eight dogs identified a 6 kb copy number variant (CNV) 152 kb upstream of KITLG. Genotyping with digital droplet PCR (ddPCR) confirmed a significant association between an increased copy number with the dark-red coat color in NSDTR (p = 6.1 &times, 10&minus, 7). The copy number of the CNV was also significantly associated with coat color variation in both eumelanin and pheomelanin-based Poodles (p = 1.5 &times, 8, 4.0 &times, 9) and across other breeds. Moreover, the copy number correlated with pigment intensity along the hair shaft in both pheomelanin and eumelanin coats. KITLG plays an important role in melanogenesis, and variants upstream of KITLG have been associated with coat color variation in mice as well as hair color in humans consistent with its role in the domestic dog.

Published
2020
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