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Your search keyword '"Tomohiro Sekiguchi"' showing total 2 results
Start Over Author "Tomohiro Sekiguchi" Publisher mdpi ag
2 results on '"Tomohiro Sekiguchi"'

1. Integrated On-Chip 3D Vascular Network Culture under Hypoxia

Author

Miguel Ángel Olmedo-Suárez, Tomohiro Sekiguchi, Atsushi Takano, Maria del Pilar Cañizares-Macías, and Nobuyuki Futai

Subjects
3D cell culture, hypoxia, angiogenesis, portable cell culture device, vascular network, normoxic/hypoxic transition, Mechanical engineering and machinery, TJ1-1570
Abstract

We developed a portable device made of poly(dimethylsiloxane) (PDMS)/polymethylmethacrylate (PMMA) for long-term 3D cell culture of vascular endothelial cells for the development of a vascular network and evaluated the device under different transitions between normoxia and hypoxia with good optical accessibility. The combination of a nested reservoir device and a bicarbonate/ascorbate buffer system accomplished on-chip incubation with 4.91 ± 0.86% pO2 and 5.19 ± 1.70% pCO2 for up to 10 days. Seventy-two hours of normoxic incubation preceding hypoxic culture increased the cell viability, network formation, and size and stability of the resulting lumens compared with those completely maintained in normoxia for the same total duration. We employed different parameters of the network (e.g., total mesh area, total length, number of branches, among others) for the comparison of different oxygen treatments in the device. The differential effect of hypoxic conditions based on the maturity of the vessels may be used as an external factor to improve vascular development in vitro.

Published
2020
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2. Integrated On-Chip 3D Vascular Network Culture under Hypoxia

Author

Atsushi Takano, María P. Cañizares-Macías, Nobuyuki Futai, Tomohiro Sekiguchi, and Miguel Ángel Olmedo-Suárez

Subjects
Angiogenesis, lcsh:Mechanical engineering and machinery, Bicarbonate, chemistry.chemical_element, 02 engineering and technology, Oxygen, Article, pCO2, angiogenesis, 03 medical and health sciences, chemistry.chemical_compound, 3D cell culture, medicine, lcsh:TJ1-1570, vascular network, Viability assay, Electrical and Electronic Engineering, Incubation, 030304 developmental biology, 0303 health sciences, hypoxia, Mechanical Engineering, portable cell culture device, Hypoxia (medical), 021001 nanoscience & nanotechnology, chemistry, Control and Systems Engineering, normoxic/hypoxic transition, medicine.symptom, 0210 nano-technology, Biomedical engineering
Abstract

We developed a portable device made of poly(dimethylsiloxane) (PDMS)/polymethylmethacrylate (PMMA) for long-term 3D cell culture of vascular endothelial cells for the development of a vascular network and evaluated the device under different transitions between normoxia and hypoxia with good optical accessibility. The combination of a nested reservoir device and a bicarbonate/ascorbate buffer system accomplished on-chip incubation with 4.91 &plusmn, 0.86% pO2 and 5.19 &plusmn, 1.70% pCO2 for up to 10 days. Seventy-two hours of normoxic incubation preceding hypoxic culture increased the cell viability, network formation, and size and stability of the resulting lumens compared with those completely maintained in normoxia for the same total duration. We employed different parameters of the network (e.g., total mesh area, total length, number of branches, among others) for the comparison of different oxygen treatments in the device. The differential effect of hypoxic conditions based on the maturity of the vessels may be used as an external factor to improve vascular development in vitro.

Published
2020
Full Text
View/download PDF

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