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This work determines the deactivation mechanisms of Cu/ZSM-5 catalysts used for the conversion of 2,3-butanediol to butene as part of an alcohol-to-jet route. The deactivation of the catalyst, reflected by a drop in the rate of the limiting hydrogenation step by over 90% in 24 h at a weight hourly space velocity of 5.92 h−1, proceeds via both the agglomeration of copper particles and the obstruction of copper surfaces due to carbonaceous deposits, although the former has less impact on the decrease in the hydrogenation rate. To reduce the detrimental effect of carbonaceous deposits on catalytic activity, ZMS-5 is modified through desilication of the HZSM-5 support with NaOH and CsOH solutions to generate a hierarchical structure with mesopores. The catalyst with the CsOH-treated support generates the highest overall yield of desired olefin products and experiences the slowest deactivation. This is a result of the lower Brønsted acidity and larger mesopores found in the CsOH-treated catalyst, leading to the slower formation of carbonaceous deposits and the faster diffusion of their precursors out of the pores.

The use of gas diffusion electrodes (GDEs) in microbial fuel cells (MFCs) can improve their cell performance, but tends to cause fouling. In order to allow long-term stable operation, the search for antifouling methods is necessary. Therefore, an antibacterial coating with ammonium compounds is investigated. Within the first 30 days of operation, the maximum measured power density of a GDE with antibacterial ionomer was 606 mW m−2. The GDE without an antifouling treatment could only reach a maximum of 284 mW m−2. Furthermore, there was an optimum in the loading amount with ionomer below 2.6 mg cm−2. Further investigations showed that additional aeration of the GDEs by a fan had a negative effect on their performance. Despite the higher performance, the antibacterial coating could not prevent biofilm growth at the surface of the GDE. The thickness of the biofilm was only reduced by 14–16%. However, the weight of the biofilm on the treated GDEs was 62–80% less than on a GDE without an antifouling treatment. Consequently, the coating cannot completely prevent fouling, but possibly leads to a lower density of the biofilm or prevents clogging of the pores inside the electrodes and improves their long-term stability.

Demand-Responsive Transport (DRT) is one of the most valid solutions to tackle the problems affecting public transport today, both in urban and rural areas. Despite its undoubted advantages, it still remains underdeveloped compared to its great potential. The purpose of this paper is to understand the role that on-demand transport plays in the strategic choices of public transport authorities (PTAs): to this end, this study examined the DRT services of three geographically distant European cities, in order to test different social, cultural and regulatory backgrounds, examining their main characteristics. Tampere, Braunschweig and Genoa were selected for the purposes of this work; data and information were collected by viewing the official websites of public transport companies and by direct contact via mail/telephone with the managers responsible for on-call transport. The data collected were then analyzed based on specific Key Performance Indicators (KPIs) identified in academic literature. The results of this paper show that the role of on-call service in the strategic decisions of PTAs depends on the cultural context of reference; some cities focus more on urban services, others on rural transport. In all three case studies examined, on-demand transport is an important aspect of local mobility and with wide room for growth.

Chronic progressive lymphedema (CPL) is a prevalent and progressive disease in Rhenish German draught horses. The objective of our follow-up study was to evaluate the heritability of this disease in Rhenish German draught horses using pedigree-based and genomic relationship matrices. We employed linear and threshold animal models. Models included the random animal effect and effects of breeding association, coat colour, sex, and age within sex, and farm-related factors, on CPL scores. In addition, we estimated heritabilities in models assuming censoring for data when horses were below an age of 1–15 years. The heritabilities of CPL scores across all ages were 0.595 ± 0.131 and 0.482 ± 0.105 in the threshold and linear animal model with pedigree-based relationship matrices, respectively. The restriction of data to horses with a minimum age at examination or accounting for censored data in younger animals showed an increase in heritabilities of CPL scores up to 0.788 ± 0.168 (threshold model) and 0.752 ± 0.153 (linear model) at an age of 7–8 years. Analyses including genomic relationship matrices yielded very similar estimates, but with smaller standard errors than pedigree-based analyses. Heritabilities in threshold models for CPL prevalence (CPL-bin-score) and the number of affected limbs (CPL-bin-sum) were 0.176–0.189 ± 0.061–0.064 and 0.375–0.433 ± 0.164–0.170, respectively. We were able to show moderately to highly positive genetic correlations between the CPL score and cannon bone circumference (0.529–0.825), height at withers (0.338–0.555), and skinfold thickness (0.241–0.517). Using the dichotomous trait for the CPL score and the genomic relationship matrix resulted in corresponding estimates of 0.868, 0.793, and 0.784, respectively. This study showed the great importance of additive genetic variation influencing the expression of chronic progressive lymphedema in Rhenish German draught horses. Therefore, further research is warranted to implement breeding programmes in a small breeding population that exploit the potential of additive genetic differences among animals for reducing the prevalence and severity of lesions of this incurable disease.

While benthic characteristics of coral reef habitats are a major driver of the structure of coral reef fish assemblages, non-reef habitats adjacent to coral reefs (e.g., mangroves, seagrass beds, and macroalgal beds) can affect reef fish assemblages. Here, we investigate how reef fish assemblages respond to local-scale benthic habitats within a coral reef and larger-scale adjacent seascape features (habitats within 500 m of coral reefs) on Siquijor Island in the Philippines. We examined an abundance of species for the entire reef fish assemblage and within the assemblages of parrotfishes (subfamily Scarinae) and wrasses (family Labridae). Five distinct habitat types were identified in a cluster analysis, which incorporated benthic characteristics within coral reefs and habitats adjacent to coral reefs. We found that the diversity and structure of coral reef fish assemblages were affected by benthic characteristics within coral reefs and also by benthic habitat types adjacent to coral reefs. Individual species responses and juveniles of certain species demonstrated uniquely high abundances in habitat clusters characterized by the non-reef habitats surrounding coral reefs. Considering coral reef habitats and adjacent non-reef habitats as a holistic, interconnected seascape will provide better estimations of the drivers of the structures of coral reef fish assemblages.

Reverse engineering is applied to identify optimum polymerization conditions for the synthesis of polymers with pre-defined properties. The proposed approach uses multi-objective optimization (MOO) and provides multiple candidate polymerization procedures to achieve the targeted polymer property. The objectives for optimization include the maximal similarity of molar mass distributions (MMDs) compared to the target MMDs, a minimal reaction time, and maximal monomer conversion. The method is tested for vinyl acetate radical polymerizations and can be adopted to other monomers. The data for the optimization procedure are generated by an in-house-developed kinetic Monte-Carlo (kMC) simulator for a selected recipe search space. The proposed reverse engineering algorithm comprises several steps: kMC simulations for the selected recipe search space to derive initial data, performing MOO for a targeted MMD, and the identification of the Pareto optimal space. The last step uses a weighted sum optimization function to calculate the weighted score of each candidate polymerization condition. To decrease the execution time, clustering of the search space based on MMDs is applied. The performance of the proposed approach is tested for various target MMDs. The suggested MOO-based reverse engineering provides multiple recipe candidates depending on competing objectives.

In GEMINI-1/-2, dolutegravir + lamivudine was non-inferior to dolutegravir + tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) in achieving viral suppression (viral load [VL] < 50 copies/mL) in treatment-naive adults. Abbott’s RealTime HIV-1 assay provides quantitative VL (40–10,000,000 copies/mL) and qualitative target detected or target not detected (TND) for VL < 40 copies/mL. This post hoc analysis assessed very-low-level viremia and “blips” through Week 144. Proportions with VL < 40 copies/mL and TND are presented overall and by baseline VL and CD4+ cell count. “Blips” (single VL ≥ 50 to

Following Max Liboiron’s claim that pollution is colonialism, the anti-colonial maritime archaeologist’s role in the Anthropocene might be to reframe research questions, so that focus is directed toward interactions between marine and maritime, and that the colonial ‘resurrectionist’ approach that has dominated nautical archaeology ought to be reconsidered altogether. This normative statement is put to the test with a 4000-year-old waterlogged dugout canoe that was illegally excavated from the Cooper River in South Carolina, USA. Upon retrieval, the affected tribal entities were brought into consultation with archaeologists and conservators to help decide how to proceed with the canoe’s remains. Tribal representatives reached a consensus to preserve the canoe with PEG and display it in a public museum. This procedure follows the resurrectionist model typical of maritime archaeology in the West, now the dominant protocol globally, where the scholar acts as savior by lifting entire wrecks from watery graves and promising to grant them immortality in utopian museum spaces. However, this immortalizing procedure is at odds with some Indigenous values, voiced by tribal representatives, which embrace life cycles and distributed agency. In the end, the desire to preserve the canoe as a perpetual symbol of intertribal unity dominated concerns surrounding the canoe’s own life, spirit, and autonomy, and that plasticizing it would permanently alter its substance and essence. We argue that the object of the canoe has become subservient to its postcolonial symbolism of Indigenous unity, resilience, and resistance. Further, by subscribing to the resurrectionist model of maritime archaeology, the immortalized canoe now bears the irony of colonial metaphor, as an unintended consequence of its preservation. We echo Audre Lorde’s famous sentiment by wondering if an anticolonial maritime archaeology can ever hope to dismantle the master’s boat using the master’s tools. The conclusions reached here have implications for other maritime and museum contexts too, including the highly publicized case of the wrecked 1859–1860 slave ship, Clotilda.

This manuscript is based on cooperation between the universities in Tampere, Finland and Braunschweig, Germany. New tram lines are being built or extended in both cities to provide better connections to public transportation and increase accessibility. This research presents case studies from each city that have successfully increased public transport (PT) ridership during recent years. The aim of this study is to compare and analyse the effects of tram expansion projects in Tampere and Braunschweig from the public transport network users’ viewpoint. These cities were selected because they are of similar size in terms of population and have similar modal shares of PT. Changes in accessibility to the city centres were analysed considering the tramline extensions. This was determined by calculating the travel time differences of the current network and the future network for both cities. For this total travel time, the ArcGIS Pro Service Area Tool was used to calculate the time taken to reach every grid of the investigation areas. The results vary due to the tram expansion and changes in the existing bus network.

Background and Objectives: Patients with congenital heart disease (CHD), especially as a concomitant syndromal disease of trisomy 21 (T21), are at risk for impaired neurodevelopment. This can also affect these patients’ education. However, there continues to be a research gap in the educational development of CHD patients and T21 CHD patients. Materials and Methods: In total, data from 2873 patients from the German National Register for Congenital Heart Defects were analyzed. The data are based on two online education surveys conducted among patients registered in the National Register for Congenital Heart Defects (2017, 2020). Results: Of 2873 patients included (mean age: 14.1 ± 4.7 years, 50.5% female), 109 (3.8%) were identified with T21 (mean age: 12.9 ± 4.4 years, 49.5% female). T21 CHD participants had a high demand for early specific interventions (overall cohort 49.1%; T21 cohort 100%). T21 CHD children more frequently attended special schools and, compared to non-trisomy 21 (nT21) CHD patients, the probability of attending a grammar school was reduced. In total, 87.1% of nT21 CHD patients but 11% of T21 CHD patients were enrolled in a regular elementary school, and 12.8% of T21 CHD patients could transfer to a secondary school in contrast to 35.5% of nT21 CHD patients. Most of the T21 CHD patients were diagnosed with psychiatric disorders, e.g., learning, emotional, or behavioral disorders (T21 CHD patients: 82.6%; nT21 CHD patients: 31.4%; p < 0.001). Conclusions: CHD patients are at risk for impaired academic development, and the presence of T21 is an aggravating factor. Routine follow-up examinations should be established to identify developmental deficits and to provide targeted interventions.

In this article, a partial selection of experiments on enhancing the impact resistance of structural components with non-metallic, textile-reinforced concrete is discussed. The focus is on the experimental investigations in which the impact resistance of thin, textile-reinforced concrete plates is characterized. The article discusses the materials, fabrics and test setup used. For the experimental work, a drop tower from the Otto Mohr Laboratory, which belongs to the Technische Universtät Dresden, was used. Furthermore, the experimental results are presented and evaluated using different methods. Based on the collected data, a suitable approach to determining the perforation velocity of an impactor through the investigated thin, textile-reinforced concrete plates is shown.

The incidence of melanoma, being one of the most commonly occurring cancers, has been rising since the past decade. Patients at advanced stages of the disease have very poor prognoses, as opposed to at the earlier stages. The conventional targeted therapy is well defined and effective for advanced-stage melanomas for patients not responding to the standard-of-care immunotherapy. However, targeted therapies do not prove to be as effective as patients inevitably develop V-Raf Murine Sarcoma Viral Oncogene Homolog B (BRAF)-inhibitor resistance to the respective drugs. Factors which are driving melanoma drug resistance mainly involve mutations in the mitogen-activated protein kinase (MAPK) pathway, e.g., BRAF splice variants, neuroblastoma RAS viral oncogene homolog (NRAS) amplification or parallel survival pathways. However, those mechanisms do not explain all cases of occurring resistances. Therefore, other factors accounting for BRAFi resistance must be better understood. Among them there are long non-coding RNAs (lncRNAs), but these remain functionally poorly understood. Here, we conduct a comprehensive, unbiased, and integrative study of lncRNA expression, coupled with a Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-mediated activation (CRISPRa) and small molecule inhibitor screening for BRAF inhibitor resistance to expand the knowledge of potentially druggable lncRNAs, their function, and pave the way for eventual combinatorial treatment approaches targeting diverse pathways in melanoma.

Magneto-optical indicator films (MOIFs) are a very useful tool for direct studies of the spatial distribution of magnetic fields and the magnetization processes in magnetic materials and industrial devices such as magnetic sensors, microelectronic components, micro-electromechanical systems (MEMS), and others. The ease of application and the possibility for direct quantitative measurements in combination with a straightforward calibration approach make them an indispensable tool for a wide spectrum of magnetic measurements. The basic sensor parameters of MOIFs, such as a high spatial resolution down to below 1 μm combined with a large spatial imaging range of up to several cm and a wide dynamic range from 10 μT to over 100 mT, also foster their application in various areas of scientific research and industry. The history of MOIF development totals approximately 30 years, and only recently have the underlying physics been completely described and detailed calibration approaches been developed. The present review first summarizes the history of MOIF development and applications and then presents the recent advances in MOIF measurement techniques, including the theoretical developments and traceable calibration methods. The latter make MOIFs a quantitative tool capable of measuring the complete vectorial value of a stray field. Furthermore, various scientific and industrial application areas of MOIFs are described in detail.

Chronic progressive lymphedema (CPL) is a common disease in Rhenisch German draught horses. The objective of our study was to evaluate the prevalence of this disease and its progression with age in 493 Rhenish German draught horses from different regions in Germany. We employed generalized linear models with binomial, multinomial, and normal distributions to analyse the effects of breeding association, coat colour, sex and age within sex, time of examination, limb on which CPL was recorded, and farm-related factors on disease scores. The occurrence and severity of CPL were significantly influenced by breeding area, sex, coat colour, and interaction of age by sex. Males had significantly higher CPL-scores than geldings (1.92-fold) and females (5.02-fold) as well as a faster (1.85-fold) disease progression per year of life than females (1.44-fold) and geldings (1.25-fold). Regression on age within sex was still significant when age was restricted to a minimum of 7 years in the analysis. Bay horses exhibited 1.77-fold and 2.19-fold higher CPL-scores than chestnut and black horses. Keeping horses on pasture and keeping bedding clean reduced CPL-scores, whereas feeding of hay silage and concentrates during winter increased CPL-scores. Our analysis revealed significant regression coefficients of cannon bone circumference on CPL-scores. Relationships with hoof measures and skinfold diameter at the neck were not found. In conclusion, differences among breeding districts and sexes by age had the largest impact on CPL prevalence and scores. Disease progression was evident up to a mean age of 16 years in males and 20 years in females. The identification of risk factors and their effects on CPL should help to reduce the occurrence and progression of CPL.

The energy transition and the resulting expansion of renewable energy resources increasingly pose a challenge to the energy system due to their volatile and intermittent nature. In this context, energy management systems are central as they coordinate energy flows and optimize them toward economic, technical, ecological, and social objectives. While numerous scientific publications study the infrastructure, optimization, and implementation of residential energy management systems, only little research exists on industrial energy management systems. However, results are not easily transferable due to differences in complexity, dependency, and load curves. Therefore, we present a systematic literature review on state-of-the-art research for residential and industrial energy management systems to identify trends, challenges, and future research directions. More specifically, we analyze the energy system infrastructure, discuss data-driven monitoring and analysis, and review the decision-making process considering different objectives, scheduling algorithms, and implementations. Thus, based on our insights, we provide numerous recommendations for future research in residential and industrial energy management systems.

Complex functioning of the genome in the cell nucleus is controlled at different levels: (a) the DNA base sequence containing all relevant inherited information; (b) epigenetic pathways consisting of protein interactions and feedback loops; (c) the genome architecture and organization activating or suppressing genetic interactions between different parts of the genome. Most research so far has shed light on the puzzle pieces at these levels. This article, however, attempts an integrative approach to genome expression regulation incorporating these different layers. Under environmental stress or during cell development, differentiation towards specialized cell types, or to dysfunctional tumor, the cell nucleus seems to react as a whole through coordinated changes at all levels of control. This implies the need for a framework in which biological, chemical, and physical manifestations can serve as a basis for a coherent theory of gene self-organization. An international symposium held at the Biomedical Research and Study Center in Riga, Latvia, on 25 July 2022 addressed novel aspects of the abovementioned topic. The present article reviews the most recent results and conclusions of the state-of-the-art research in this multidisciplinary field of science, which were delivered and discussed by scholars at the Riga symposium.

Microbial fuel cells are a promising technology for future wastewater treatment, as it allows cleaning and power generation simultaneously. The bottleneck of microbial fuel cells is often its cathodes because they determine the power output. Gas diffusion electrodes might overcome this bottleneck due to their low production costs and high oxygen reduction rates. However, biofilm formation on the gas diffusion electrodes reduces their performance over time. In this work, a new reactor design of the microbial fuel cell using rotating gas diffusion electrodes is presented. The biofilm growth on the electrode during operation was observed and its effect on the performance of the microbial fuel cell was examined. In addition, different antifouling strategies were investigated over a period of 80 days. It was found that already after 7 days of operation a complete biofilm had grown on an untreated gas diffusion electrode. However, this does not seem to affect the performance of the cells in the beginning. Differences in the performance of the reactors with and without an antifouling strategy only become apparent from day 15 onwards. The use of UV radiation and antibacterial membranes leads to the best results with maximum power densities of approx. 200 mW m−2 while the untreated microbial fuel cell only achieves a maximum power density of approx. 20 mW m−2.

A system of boron-doped diamond (BDD) anode combined with a gas diffusion electrode (GDE) as a cathode is an attractive kind of electrolysis system to treat wastewater to remove organic pollutants. Depending on the operating parameters and water matrix, the kinetics of the electrochemical reaction must be defined to calculate the reaction rate constant, which enables designing the treatment reactor in a continuous process. In this work, synthetic wastewater simulating the vacuum toilet sewage on trains was treated via a BDD-GDE reactor, where the kinetics was presented as the abatement of chemical oxygen demand (COD) over time. By investigating three different initial COD concentrations (C0,1 ≈ 2 × C0,2 ≈ 4 × C0,3), the kinetics was presented and the observed reaction rate constant kobs. was derived at different current densities (20, 50, 100 mA/cm2). Accordingly, a mathematical model has derived kobs. as a function of the cell potential Ecell. Ranging from 1 × 10−5 to 7.4 × 10−5 s−1, the kobs. is readily calculated when Ecell varies in a range of 2.5–21 V. Furthermore, it was experimentally stated that the highest economic removal of COD was achieved at 20 mA/cm2 demanding the lowest specific charge (~7 Ah/gCOD) and acquiring the highest current efficiency (up to ~48%).

AISI 347 austenitic steel is, as an example, used in nuclear energy piping systems. Piping filled with superheated steam or cooled water is particularly exposed to high stresses, whereupon local material properties in the pipes can change significantly, especially in the case of additional corrosive influences, leading to aging of the material. In the absence of appropriate information, such local material property variations are currently covered rather blanketly by safety factors set during the design of those components. An increase in qualified information could improve the assessment of the condition of such aged components. As part of the collaborative project “Microstructure-based assessment of the maximum service life of core materials and components subjected to corrosion and fatigue (MiBaLeB)”, the short-time procedure, StrainLife, was developed and validated by several fatigue tests. With this procedure, a complete S–N curve of a material can be determined on the basis of three fatigue tests only, which reduces the effort compared to a conventional approach significantly and is thus ideal for assessing the condition of aged material, where the material is often rare, and a cost-effective answer is often very needed. The procedure described is not just limited to traditional parameters, such as stress and strain, considered in destructive testing but rather extends into parameters derived from non-destructive testing, which may allow further insight into what may be happening within a material’s microstructure. To evaluate the non-destructive quantities measured within the StrainLife procedure and to correlate them with the aging process in a material, several fatigue tests were performed on unnotched and notched specimens under cyclic loading at room and elevated temperatures, as well as under various media conditions, such as distilled water and reactor pressure vessel boiling water (BWR) conditions.

The assessment of metallic materials used in power plants’ piping represents a big challenge due to the thermal transients and the environmental conditions to which they are exposed. At present, a lack of information related to degradation mechanisms in structures and materials is covered by safety factors in its design, and in some cases, the replacement of components is prescribed after a determined period of time without knowledge of the true degree of degradation. In the collaborative project “Microstructure-based assessment of maximum service life of nuclear materials and components exposed to corrosion and fatigue (MibaLeb)”, a methodology for the assessment of materials’ degradation is being developed, which combines the use of NDT techniques for materials characterization, an optimized fatigue lifetime analysis using short time evaluation procedures (STEPs) and numerical simulations. In this investigation, the AISI 347 (X6CrNiNb18-10) is being analyzed at different conditions in order to validate the methodology. Besides microstructural analysis, tensile and fatigue tests, all to characterize the material, a pressurized hot water pipe exposed to a series of flow conditions will be evaluated in terms of full-scale testing as well as prognostic evaluation, where the latter will be based on the materials’ data generated, which should prognose changes in the material’s condition, specifically in a pre-cracked stage. This paper provides an overview of the program, while the more material’s related aspects are presented in the subsequent paper.

This study scrutinizes management and clinical presentation of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) in pediatric inpatient care and evaluates the utilization of pediatric healthcare capacity during the pandemic. Within this retrospective cohort study, we systematically reviewed data of all 16,785 pediatric patients (

Magnetic force microscopy (MFM) is a widespread technique for imaging magnetic structures with a resolution of some 10 nanometers. MFM can be calibrated to obtain quantitative (qMFM) spatially resolved magnetization data in units of A/m by determining the calibrated point spread function of the instrument, its instrument calibration function (ICF), from a measurement of a well-known reference sample. Beyond quantifying the MFM data, a deconvolution of the MFM image data with the ICF also corrects the smearing caused by the finite width of the MFM tip stray field distribution. However, the quality of the calibration depends critically on the calculability of the magnetization distribution of the reference sample. Here, we discuss a Ti/Pt/Co multilayer stack that shows a stripe domain pattern as a suitable reference material. A precise control of the fabrication process, combined with a characterization of the sample micromagnetic parameters, allows reliable calculation of the sample’s magnetic stray field, proven by a very good agreement between micromagnetic simulations and qMFM measurements. A calibrated qMFM measurement using the Ti/Pt/Co stack as a reference sample is shown and validated, and the application area for quantitative MFM measurements calibrated with the Ti/Pt/Co stack is discussed.

Cracking of propyl side chains from 4-propylphenol, a model compound for lignin monomers, is studied for a commercial ZSM-5 zeolite catalyst. The decline of 4-propylphenol conversion with time on stream can be delayed by co-feeding water. FTIR spectroscopy shows the formation of chemisorbed phenolates during reactions and significant amounts of phenolics are detected by GC-MS of the extract from the spent catalysts. Thus, chemisorbed phenolates are identified as the main reason for deactivation in the absence of water. Regardless of the amount of co-fed water, substituted monoaromatics and polyaromatic species are formed. Comprehensive characterization of the spent catalysts including Raman and solid-state 27Al NMR spectroscopy, and thermogravimetric analysis points to a combination of deactivation processes. First, phenolates bind to Lewis acid sites within the zeolite framework and hinder diffusion unless they are hydrolyzed by water. In addition, light olefins created during the cracking process react to form a polyaromatic coke that deactivates the catalyst more permanently.

Juvenile fish often use alternative habitats distinct from their adult phases. Parrotfishes are an integral group of coral reef fish assemblages, are targeted in fisheries, are sensitive to reef disturbances, and have been documented as multiple-habitat users. Considering the abundance of research conducted on parrotfishes, very little is known about their juvenile ecology at the species level due to their cryptic and variable coloration patterns. We collected juvenile parrotfishes in non-reef habitats (macroalgal beds, seagrass beds, and lagoons) in the Philippines and used DNA analysis to determine species composition. The results were then compared with data on adult parrotfish abundance from underwater visual census (UVC) surveys in coral reef and non-reef habitats. Collections identified 15 species of juvenile parrotfishes in non-reef habitats, and of these, 10 were also recorded in UVCs as adults. Informed by adult surveys, 42% of the 19 parrotfish species observed as adults were classified as multi-habitat users based on their presence in coral reef and non-reef habitats. When accounting for the occurrence of species as juveniles in non-reef habitats, 93% of the species collected as juveniles would be considered multi-habitat users. Species identified as juveniles in non-reef habitats comprised 50% of the average adult parrotfish density on coral reefs and 58–94% in non-reef habitats. The species richness of juveniles in non-reef habitats was greater than that of adults occupying the same habitats, and the most common adult species observed in UVCs was not collected as juveniles in non-reef habitats. Finally, UVC suggested that 97% of juvenile parrotfish

Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was the least-reported serotype in Indonesia during the last five decades. We recently conducted a molecular epidemiology study of dengue in the Jember regency, East Java province, Indonesia. Dengue is endemic in the region and outbreaks occur annually. We investigated the clinical characteristics and etiology of dengue-like febrile illness in this regency to understand the disease dynamics. A total of 191 patients with clinical symptoms similar to dengue were recruited during an 11-month study in 2019–2020. Children accounted for the majority of cases and dengue burden was estimated in 41.4% of the cases based on NS1 antigen, viral RNA, and IgG/IgM antibody detection with the majority (73.4%) being primary infections. Secondary infection was significantly associated with a higher risk of severe dengue manifestation. All four DENV serotypes were detected in Jember. Strikingly, we observed the predominance of DENV-4, followed by DENV-3, DENV-1, and DENV-2. Genotype determination using Envelope gene sequence revealed the classification into Genotype I, Cosmopolitan Genotype, Genotype I, and Genotype II for DENV-1, -2, -3, and -4, respectively. The predominance of DENV-4 in Jember may be associated with a new wave of DENV infections and spread in a non-immune population lacking a herd-immunity to this particular serotype.

Empirical models could help us to understand the process of plant residue decomposition and nutrient release into the soil. The objective of this study was to determine an appropriate model to describe the decomposition of hairy vetch (Vicia villosa Roth) and cereal rye (Secale cereale L.) cover crop (CC) residue and nitrogen (N) release. Data pertaining to above and belowground CC residue mass loss and N release for up to 2633 cumulative decomposition degree days (112 d) after litterbag installation were obtained from two cropping system experiments, a 1-yr study conducted in 2015 and a 2-yr study during 2017 to 2018 in the humid subtropical environment of southern IL, USA. Six exponential and two hyperbolic models were fit to percent mass and N remaining data to find the one with minimum Akaike information criterion (AIC) and residual sum of squares. Modified three-parameter single exponential and two- or three-parameter hyperbolic models best met the assumed criteria of selection for above and belowground CC residue, respectively. Fitting a double exponential model to combined data for percent mass and N remaining identified two mass and N pools, a fast and a slow pool with different rate constants. A five-parameter double exponential with an asymptote met the preset criteria and passed all tests for normally distributed population, constant variance, and independence of residuals at α = 0.05 when fit to combined data of hairy vetch shoot mass and N remaining. However, a two-parameter hyperbolic and three-parameter asymptotic hyperbolic model provided the best fit to a combined data of cereal rye shoot mass and N remaining, respectively. Both hyperbolic decay models showed a good fit for belowground mass decomposition and N release for both CCs. Cereal rye had a poorer fit than hairy vetch for mass and N remaining of both above and belowground mass. The best-selected decay models can be used to estimate the decomposition and N release rates of hairy vetch and cereal rye above and belowground residue in a similar environment.

When optimizing chimeric antigen receptor (CAR) therapy in terms of efficacy, safety, and broadening its application to new malignancies, there are two main clusters of topics to be addressed: the CAR design and the choice of transfected cells. The former focuses on the CAR construct itself. The utilized transmembrane and intracellular domains determine the signaling pathways induced by antigen binding and thereby the cell-specific effector functions triggered. The main part of this review summarizes our understanding of common signaling domains employed in CARs, their interactions among another, and their effects on different cell types. It will, moreover, highlight several less common extracellular and intracellular domains that might permit unique new opportunities. Different antibody-based extracellular antigen-binding domains have been pursued and optimized to strike a balance between specificity, affinity, and toxicity, but these have been reviewed elsewhere. The second cluster of topics is about the cellular vessels expressing the CAR. It is essential to understand the specific attributes of each cell type influencing anti-tumor efficacy, persistence, and safety, and how CAR cells crosstalk with each other and bystander cells. The first part of this review focuses on the progress achieved in adopting different leukocytes for CAR therapy.

This work presents a computationally efficient predictive model based on solid heat transfer for temperature profiles in powder bed metal additive manufacturing (PBMAM) considering the heat transfer boundary condition and powder material properties. A point moving heat source model is used for the three-dimensional temperature prediction in an absolute coordinate. The heat loss from convection and radiation is calculated using a heat sink solution with a mathematically discretized boundary considering non-uniform temperatures and heat loss at the boundary. Powder material properties are calculated considering powder size statistical distribution and powder packing. The spatially uniform and temperature-independent material properties are employed in the temperature prediction. The presented model was tested in PBMAM of AlSi10Mg under different process conditions. The calculations of material properties are needed for AlSi10Mg because of the significant difference in thermal conductivity between powder form and solid bulk form. Close agreement is observed upon experimental validation on the molten pool dimensions.

As systems continue to grow in scale and complexity and have to operate safely in challenging disruptive environments, system safety and resilience has become a critical requirement. This recognition has drawn attention to the concept of resilience, which has different definitions and several different interpretations that tend to be domain specific. For example, resilience in health care clinics means something quite different than resilience in self-driving cars, or energy grids. This paper reviews the different characterizations of resilience and assesses their value proposition in realizing engineered resilient systems. This paper emphasizes the importance of systems modeling in engineering resilient systems and presents an overarching methodology that employs different modeling approaches for operational tasks as a function of problem context. This paper specifically focuses on systems modeling in partially observable and potentially hostile environments. It discusses the need for system model verification, which is key to safety, and system flexibility and adaptability, which are key to resilience. It introduces a formal, probabilistic modeling construct called the “resilience contract.” This construct employs a state-based representation that formalizes the concept of resilience while enabling system model verification and affording requisite flexibility for adaptation and learning. The key findings of our research are that different system modeling approaches and algorithms are needed based on mission tasks and operational context; adaptive capacity and continual adaptability are the two promising characterizations of resilience that can be cost-effectively realized in real-world systems; and the resilience contract construct is an effective means for probabilistic verification of system model correctness while affording flexibility needed for adaptation and learning. Collectively, these findings contribute to the body of knowledge in both model-based systems engineering (MBSE) and engineered resilient systems.

In recent years, the use of whole-cell biocatalysts and biocatalytic enzymes in biotechnological applications originating from the genus Pseudomonas has greatly increased. In 2014, two new species within the Pseudomonas putida group were isolated from Swiss forest soil. In this study, the high quality draft genome sequences of Pseudomonas wadenswilerensis CCOS 864T and Pseudomonas reidholzensis CCOS 865T were used in a comparative genomics approach to identify genomic features that either differed between these two new species or to selected members of the P. putida group. The genomes of P. wadenswilerensis CCOS 864T and P. reidholzensis CCOS 865T were found to share genomic features for the degradation of aromatic compounds or the synthesis of secondary metabolites. In particular, genes encoding for biocatalytic relevant enzymes belonging to the class of oxidoreductases, proteases and isomerases were found, that could yield potential applications in biotechnology. Ecologically relevant features revealed that both species are probably playing an important role in the degradation of soil organic material, the accumulation of phosphate and biocontrol against plant pathogens.

Metal additive manufacturing can produce geometrically complex parts with effective cost. The high thermal gradients due to the repeatedly rapid heat and solidification cause defects in the produced parts, such as cracks, porosity, undesired residual stress, and part distortion. Different techniques were employed for temperature investigation. Experimental measurement and finite element method-based numerical models are limited by the restricted accessibility and expensive computational cost, respectively. The available physics-based analytical model has promising short computational efficiency without resorting to finite element method or any iteration-based simulations. However, the heat transfer boundary condition cannot be considered without the involvement of finite element method or iteration-based simulations, which significantly reduces the computational efficiency, and thus the usefulness of the developed model. This work presents an explicit and closed-form solution, namely heat sink solution, to consider the heat transfer boundary condition. The heat sink solution was developed from the moving point heat source solution based on heat transfer of convection and radiation. The part boundary is mathematically discretized into many heats sinks due to the non-uniform temperature distribution, which causes non-uniform heat loss. The temperature profiles, thermal gradients, and temperature-affected material properties are calculated and presented. Good agreements were observed upon validation against experimental molten pool measurements.

The global burden of chronic kidney disease is rising. The etiologies, heterogeneous, and arterial hypertension, are key factors contributing to the development and progression of chronic kidney disease. Arterial hypertension is induced and maintained by a complex network of systemic signaling pathways, such as the hormonal axis of the renin-angiotensin-aldosterone system, hemodynamic alterations affecting blood flow, oxygen supply, and the immune system. This review summarizes the clinical and histopathological features of hypertensive kidney injury and focusses on the interplay of distinct systemic signaling pathways, which drive hypertensive kidney injury in distinct cell types of the kidney. There are several parallels between hypertension-induced molecular signaling cascades in the renal epithelial, endothelial, interstitial, and immune cells. Angiotensin II signaling via the AT1R, hypoxia induced HIFα activation and mechanotransduction are closely interacting and further triggering the adaptions of metabolism, cytoskeletal rearrangement, and profibrotic TGF signaling. The interplay of these, and other cellular pathways, is crucial to balancing the injury and repair of the kidneys and determines the progression of hypertensive kidney disease.

Temperature distribution gradient in metal powder bed additive manufacturing (MPBAM) directly controls the mechanical properties and dimensional accuracy of the build part. Experimental approach and numerical modeling approach for temperature in MPBAM are limited by the restricted accessibility and high computational cost, respectively. Analytical models were reported with high computational efficiency, but the developed models employed a moving coordinate and semi-infinite medium assumption, which neglected the part dimensions, and thus reduced their usefulness in real applications. This paper investigates the in-process temperature in MPBAM through analytical modeling using a stationary coordinate with an origin at the part boundary (absolute coordinate). Analytical solutions are developed for temperature prediction of single-track scan and multi-track scans considering scanning strategy. Inconel 625 is chosen to test the proposed model. Laser power absorption is inversely identified with the prediction of molten pool dimensions. Latent heat is considered using the heat integration method. The molten pool evolution is investigated with respect to scanning time. The stabilized temperatures in the single-track scan and bidirectional scans are predicted under various process conditions. Close agreements are observed upon validation to the experimental values in the literature. Furthermore, a positive relationship between molten pool dimensions and powder packing porosity was observed through sensitivity analysis. With benefits of the absolute coordinate, and high computational efficiency, the presented model can predict the temperature for a dimensional part during MPBAM, which can be used to further investigate residual stress and distortion in real applications.