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2. Progress in Regenerative Medicine: Exploring Autologous Platelet Concentrates and Their Clinical Applications

Author

Giannotti, Laura, primary, Di Chiara Stanca, Benedetta, additional, Spedicato, Francesco, additional, Nitti, Paola, additional, Damiano, Fabrizio, additional, Demitri, Christian, additional, Calabriso, Nadia, additional, Carluccio, Maria Annunziata, additional, Palermo, Andrea, additional, Siculella, Luisa, additional, and Stanca, Eleonora, additional

Published
2023
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3. Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells

Author

Giannotti, Laura, primary, Di Chiara Stanca, Benedetta, additional, Nitti, Paola, additional, Spedicato, Francesco, additional, Damiano, Fabrizio, additional, Demitri, Christian, additional, Calabriso, Nadia, additional, Carluccio, Maria Annunziata, additional, Palermo, Andrea, additional, Ferrante, Franco, additional, Siculella, Luisa, additional, and Stanca, Eleonora, additional

Published
2023
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6. Use of CGF in Oral and Implant Surgery: From Laboratory Evidence to Clinical Evaluation

Author

Palermo, Andrea, primary, Giannotti, Laura, additional, Di Chiara Stanca, Benedetta, additional, Ferrante, Franco, additional, Gnoni, Antonio, additional, Nitti, Paola, additional, Calabriso, Nadia, additional, Demitri, Christian, additional, Damiano, Fabrizio, additional, Batani, Tiziano, additional, Lungherini, Massimo, additional, Carluccio, Maria Annunziata, additional, Rapone, Biagio, additional, Qorri, Erda, additional, Scarano, Antonio, additional, Siculella, Luisa, additional, Stanca, Eleonora, additional, and Rochira, Alessio, additional

Published
2022
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7. Analysis of the Anti-Inflammatory and Anti-Osteoarthritic Potential of Flonat Fast®, a Combination of Harpagophytum Procumbens DC. ex Meisn., Boswellia Serrata Roxb., Curcuma longa L., Bromelain and Escin (Aesculus hippocastanum), Evaluated in In Vitro Models of Inflammation Relevant to Osteoarthritis

Author

Quarta, Stefano, primary, Santarpino, Giuseppe, additional, Carluccio, Maria Annunziata, additional, Calabriso, Nadia, additional, Scoditti, Egeria, additional, Siculella, Luisa, additional, Damiano, Fabrizio, additional, Maffia, Michele, additional, Verri, Tiziano, additional, De Caterina, Raffaele, additional, and Massaro, Marika, additional

Published
2022
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8. Assessment of Subjective Well-Being in a Cohort of University Students and Staff Members: Association with Physical Activity and Outdoor Leisure Time during the COVID-19 Pandemic

Author

Quarta, Stefano, primary, Levante, Annalisa, additional, García-Conesa, María-Teresa, additional, Lecciso, Flavia, additional, Scoditti, Egeria, additional, Carluccio, Maria Annunziata, additional, Calabriso, Nadia, additional, Damiano, Fabrizio, additional, Santarpino, Giuseppe, additional, Verri, Tiziano, additional, Pinto, Paula, additional, Siculella, Luisa, additional, and Massaro, Marika, additional

Published
2022
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11. Coffee Bioactive N-Methylpyridinium Attenuates Tumor Necrosis Factor (TNF)-α-Mediated Insulin Resistance and Inflammation in Human Adipocytes

Author

Quarta, Stefano, primary, Scoditti, Egeria, additional, Carluccio, Maria Annunziata, additional, Calabriso, Nadia, additional, Santarpino, Giuseppe, additional, Damiano, Fabrizio, additional, Siculella, Luisa, additional, Wabitsch, Martin, additional, Verri, Tiziano, additional, Favari, Claudia, additional, Del Rio, Daniele, additional, Mena, Pedro, additional, De Caterina, Raffaele, additional, and Massaro, Marika, additional

Published
2021
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12. Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential

Author

Stanca, Eleonora, primary, Calabriso, Nadia, additional, Giannotti, Laura, additional, Nitti, Paola, additional, Damiano, Fabrizio, additional, Stanca, Benedetta Di Chiara, additional, Carluccio, Maria Annunziata, additional, De Benedetto, Giuseppe Egidio, additional, Demitri, Christian, additional, Palermo, Andrea, additional, Ferrante, Franco, additional, Siculella, Luisa, additional, and Rochira, Alessio, additional

Published
2021
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14. Angiogenic Properties of Concentrated Growth Factors (CGFs): The Role of Soluble Factors and Cellular Components

Author

Calabriso, Nadia, primary, Stanca, Eleonora, additional, Rochira, Alessio, additional, Damiano, Fabrizio, additional, Giannotti, Laura, additional, Di Chiara Stanca, Benedetta, additional, Massaro, Marika, additional, Scoditti, Egeria, additional, Demitri, Christian, additional, Nitti, Paola, additional, Palermo, Andrea, additional, Siculella, Luisa, additional, and Carluccio, Maria Annunziata, additional

Published
2021
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16. IgM and IgG Profiles Reveal Peculiar Features of Humoral Immunity Response to SARS-CoV-2 Infection

Author

De Donno, Antonella, primary, Lobreglio, Giambattista, additional, Panico, Alessandra, additional, Grassi, Tiziana, additional, Bagordo, Francesco, additional, Bozzetti, Maria Pia, additional, Massari, Serafina, additional, Siculella, Luisa, additional, Damiano, Fabrizio, additional, Guerra, Francesco, additional, Greco, Marilena, additional, Chicone, Michele, additional, Lazzari, Roberta, additional, and Alifano, Pietro, additional

Published
2021
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19. Release of VEGF from Dental Implant Surface (IML® Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations

Author

Palermo, Andrea, primary, Ferrante, Franco, additional, Stanca, Eleonora, additional, Damiano, Fabrizio, additional, Gnoni, Antonio, additional, Batani, Tiziano, additional, Carluccio, Maria Annunziata, additional, Demitri, Christian, additional, and Siculella, Luisa, additional

Published
2019
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22. Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells

Author

Laura Giannotti, Benedetta Di Chiara Stanca, Paola Nitti, Francesco Spedicato, Fabrizio Damiano, Christian Demitri, Nadia Calabriso, Maria Annunziata Carluccio, Andrea Palermo, Franco Ferrante, Luisa Siculella, Eleonora Stanca, Giannotti, Laura, DI CHIARA STANCA, Benedetta, Nitti, Paola, Spedicato, Francesco, Damiano, Fabrizio, Demitri, Christian, Calabriso, Nadia, Annunziata Carluccio, Maria, Palermo, Andrea, Ferrante, Franco, Siculella, Luisa, and Stanca, Eleonora

Subjects
CGF, blood-derived biomaterials, growth factors, hydroxyapatite silicon scaffold, osteogenic differentiation, tissue regeneration, General Immunology and Microbiology, blood-derived biomaterials, CGF, growth factors, osteogenic differentiation, hydroxyapatite–silicon scaffold, tissue regeneration, General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology
Abstract

Simple Summary: The aim of this study was to identify new and innovative strategies to improve the tissue-regeneration process. Concentrated growth factor (CGF) is an autologous biomaterial rich in growth factors and multipotent stem cells. The purpose of our study was to evaluate the osteogenic differentiation of CGF primary cells in the presence of a hydroxyapatite–silicon scaffold, which represents a very interesting material in the field of bone reconstructive surgery. Our findings showed that the hydroxyapatite–silicon scaffold provided support to primary CGF cells by enhancing osteogenic differentiation. These data suggest interesting perspectives in the use of CGF together with scaffolds in the field of regenerative medicine. Abstract: The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HASi scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HASi scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration.

Published
2023

23. Mechanical and Biological Properties of Magnesium- and Silicon-Substituted Hydroxyapatite Scaffolds

Author

Alessio Rochira, Paola Nitti, Luisa Siculella, Christian Demitri, Sanosh Kunjalukkal Padmanabhan, Eleonora Stanca, Antonio Licciulli, Marta Madaghiele, Maria Grazia Raucci, Kunjalukkal Padmanabhan, Sanosh, Nitti, Paola, Stanca, Eleonora, Rochira, Alessio, Siculella, Luisa, Raucci, Maria Grazia, Madaghiele, Marta, Licciulli, Antonio, and Demitri, Christian

Subjects
Scaffold, Technology, Silicon, Biocompatibility, chemistry.chemical_element, scaffold, magnesium, Homogeneous distribution, Article, biocompatibility, substitution, medicine, General Materials Science, MTT assay, Porosity, Microscopy, QC120-168.85, Magnesium, QH201-278.5, hydroxyapatite, silicon, Osteoblast, Engineering (General). Civil engineering (General), TK1-9971, medicine.anatomical_structure, biocompatibility, hydroxyapatite, magnesium, scaffold, silicon, substitution, chemistry, Descriptive and experimental mechanics, Electrical engineering. Electronics. Nuclear engineering, TA1-2040, Nuclear chemistry
Abstract

Magnesium (Mg)- and silicon (Si)-substituted hydroxyapatite (HA) scaffolds were synthesized using the sponge replica method. The influence of Mg2+ and SiO44− ion substitution on the microstructural, mechanical and biological properties of HA scaffolds was evaluated. All synthesized scaffolds exhibited porosity &gt, 92%, with interconnected pores and pore sizes ranging between 200 and 800 μm. X-ray diffraction analysis showed that β-TCP was formed in the case of Mg substitution. X-ray fluorescence mapping showed a homogeneous distribution of Mg and Si ions in the respective scaffolds. Compared to the pure HA scaffold, a reduced grain size was observed in the Mg- and Si-substituted scaffolds, which greatly influenced the mechanical properties of the scaffolds. Mechanical tests revealed better performance in HA-Mg (0.44 ± 0.05 MPa), HA-Si (0.64 ± 0.02 MPa) and HA-MgSi (0.53 ± 0.01 MPa) samples compared to pure HA (0.2 ± 0.01 MPa). During biodegradability tests in Tris-HCl, slight weight loss and a substantial reduction in mechanical performances of the scaffolds were observed. Cell proliferation determined by the MTT assay using hBMSC showed that all scaffolds were biocompatible, and the HA-MgSi scaffold seemed the most effective for cell adhesion and proliferation. Furthermore, ALP activity and osteogenic marker expression analysis revealed the ability of HA-Si and HA-MgSi scaffolds to promote osteoblast differentiation.

Published
2021

24. Action of Thyroid Hormones, T3 and T2, on Hepatic Fatty Acids: Differences in Metabolic Effects and Molecular Mechanisms

Author

Alessio Rochira, Antonio Gnoni, Luisa Siculella, Fabrizio Damiano, Damiano, Fabrizio, Rochira, Alessio, Gnoni, Antonio, and Siculella, Luisa

Subjects
0301 basic medicine, Diiodothyronines, Catabolite repression, Review, 3,5,3′-triiodo-l-thyronine, lcsh:Chemistry, chemistry.chemical_compound, Gene expression, lcsh:QH301-705.5, Spectroscopy, fatty acid synthase, Fatty Acids, General Medicine, Computer Science Applications, Mitochondria, acetyl-CoA carboxylase, Biochemistry, Liver, Lipogenesis, Triiodothyronine, medicine.medical_specialty, Cell signaling, citrate carrier, Biology, Models, Biological, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Internal medicine, medicine, Animals, Humans, Physical and Theoretical Chemistry, Molecular Biology, Fatty acid synthesis, de novo lipogenesi, Fatty acid metabolism, Organic Chemistry, Lipid Metabolism, Metabolic pathway, Thyroxine, 030104 developmental biology, Endocrinology, de novo lipogenesis, chemistry, lcsh:Biology (General), lcsh:QD1-999, Basal metabolic rate, lipid lowering action, 3,5-diiodo-l-thyronine
Abstract

The thyroid hormones (THs) 3,3′,5,5′-tetraiodo-l-thyronine (T4) and 3,5,3′-triiodo-l-thyronine (T3) influence many metabolic pathways. The major physiological function of THs is to sustain basal energy expenditure, by acting primarily on carbohydrate and lipid catabolism. Beyond the mobilization and degradation of lipids, at the hepatic level THs stimulate the de novo fatty acid synthesis (de novo lipogenesis, DNL), through both the modulation of gene expression and the rapid activation of cell signalling pathways. 3,5-Diiodo-l-thyronine (T2), previously considered only a T3 catabolite, has been shown to mimic some of T3 effects on lipid catabolism. However, T2 action is more rapid than that of T3, and seems to be independent of protein synthesis. An inhibitory effect on DNL has been documented for T2. Here, we give an overview of the mechanisms of THs action on liver fatty acid metabolism, focusing on the different effects exerted by T2 and T3 on the regulation of the DNL. The inhibitory action on DNL exerted by T2 makes this compound a potential and attractive drug for the treatment of some metabolic diseases and cancer.

Published
2017

25. Nutritional and Hormonal Regulation of Citrate and Carnitine/Acylcarnitine Transporters: Two Mitochondrial Carriers Involved in Fatty Acid Metabolism

Author

Eleonora Stanca, Fabrizio Damiano, Luisa Siculella, Gabriele V. Gnoni, Anna Maria Giudetti, Giudetti, Anna Maria, Stanca, Eleonora, Siculella, Luisa, Gnoni, Gabriele Vincenzo, and Damiano, Fabrizio

Subjects
0301 basic medicine, Review, lcsh:Chemistry, chemistry.chemical_compound, Cytosol, Beta oxidation, lcsh:QH301-705.5, Spectroscopy, chemistry.chemical_classification, biology, Fatty Acids, General Medicine, Computer Science Applications, Mitochondria, Cholesterol, Biochemistry, Carnitine Acyltransferases, Lipogenesis, nutritional regulation, fatty acid synthesi, medicine.drug, hormonal regulation, Thyroid Hormones, citrate carrier, Carnitine-acylcarnitine translocase, Catalysis, Gene Expression Regulation, Enzymologic, Inorganic Chemistry, 03 medical and health sciences, Acetyl Coenzyme A, medicine, Animals, Humans, Carnitine, Physical and Theoretical Chemistry, Molecular Biology, Fatty acid synthesis, fatty acid synthesis, Fatty acid metabolism, Catabolism, Organic Chemistry, Fatty acid, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, biology.protein, β-oxidation, Carrier Proteins, carnitine/acylcarnitine translocase
Abstract

The transport of solutes across the inner mitochondrial membrane is catalyzed by a family of nuclear-encoded membrane-embedded proteins called mitochondrial carriers (MCs). The citrate carrier (CiC) and the carnitine/acylcarnitine transporter (CACT) are two members of the MCs family involved in fatty acid metabolism. By conveying acetyl-coenzyme A, in the form of citrate, from the mitochondria to the cytosol, CiC contributes to fatty acid and cholesterol synthesis; CACT allows fatty acid oxidation, transporting cytosolic fatty acids, in the form of acylcarnitines, into the mitochondrial matrix. Fatty acid synthesis and oxidation are inversely regulated so that when fatty acid synthesis is activated, the catabolism of fatty acids is turned-off. Malonyl-CoA, produced by acetyl-coenzyme A carboxylase, a key enzyme of cytosolic fatty acid synthesis, represents a regulator of both metabolic pathways. CiC and CACT activity and expression are regulated by different nutritional and hormonal conditions. Defects in the corresponding genes have been directly linked to various human diseases. This review will assess the current understanding of CiC and CACT regulation; underlining their roles in physio-pathological conditions. Emphasis will be placed on the molecular basis of the regulation of CiC and CACT associated with fatty acid metabolism.

Published
2016

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