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This study investigates the genetic determinants of seed coat color and pattern variations in cowpea (Vigna unguiculata), employing a genome-wide association approach. Analyzing a mapping panel of 296 cowpea varieties with 110,000 single nucleotide polymorphisms (SNPs), we focused on eight unique coat patterns: (1) Red and (2) Cream seed; (3) White and (4) Brown/Tan seed coat; (5) Pink, (6) Black, (7) Browneye and (8) Red/Brown Holstein. Across six GWAS models (GLM, SRM, MLM, MLMM, FarmCPU from GAPIT3, and TASSEL5), 13 significant SNP markers were identified and led to the discovery of 23 candidate genes. Among these, four specific genes may play a direct role in determining seed coat pigment. These findings lay a foundational basis for future breeding programs aimed at creating cowpea varieties aligned with consumer preferences and market requirements.

Obtaining spatial distribution information on mariculture in a low-cost, fast, and efficient manner is crucial for the sustainable development and regulatory planning of coastal zones and mariculture industries. This study, based on the Segment Anything Model (SAM) and high-resolution remote sensing imagery, rapidly extracted mariculture areas in Liaoning Province, a typical northern province in China with significant mariculture activity. Additionally, it explored the actual marine ownership data to investigate the marine use status of Liaoning Province’s mariculture. The total area of mariculture we extracted in Liaoning Province is 1052.89 km2. Among this, the area of cage mariculture is 27.1 km2, while raft mariculture covers 1025.79 km2. Through field investigations, it was determined that in the western part of Liaodong Bay, cage mariculture predominantly involves sea cucumbers. In the southern end of Dalian, the raft mariculture focuses on cultivating kelp. On the other hand, around the islands in the eastern region, the primary crop in raft mariculture is scallops, showing a significant geographical differentiation pattern. In the planned mariculture areas within Liaoning Province’s waters, the proportion of actual development and utilization is 11.2%, while the proportion approved for actual mariculture is 90.2%. This indicates a suspicion that 9.8% of mariculture is possibly in violation of sea occupation rights, which could be due to the untimely updating of marine ownership data. Based on SAM, efficient and accurate extraction of cage mariculture can be achieved. However, the extraction performance for raft mariculture is challenging and remains unsatisfactory. Manual interpretation is still required for satisfactory results in this context.

This study aimed to investigate the impact of Bacillus subtilis HC6 on the growth performance, immunity, antioxidant capacity, and intestinal health of broilers. A total of 180 one-day-old white feather broilers were randomly divided into two experimental groups, each comprising six replicates of fifteen chicks from 1 to 50 d of age. The groups were either fed a basal diet (CON) or the same diet supplemented with 5 × 108 cfu/kg of Bacillus subtilis HC6 (BS). Our results indicated that compared with the CON, dietary supplementation with BS increased feed efficiency during d 21–50 and d 1–50 (p < 0.05). Moreover, BS supplementation enhanced antioxidant capacity in the serum and liver, and also decreased the activity of diamine oxidase and the level of endotoxins (p < 0.05). Additionally, BS treatment increased the villi height in the jejunum and ileum, increased the ratio of villus height/crypt depth in the ileum, upregulated the expression of tight junction proteins in the jejunal mucosa, and downregulated the levels of IL-22 and IFN-γ on day 50 (p < 0.05). Principal coordinates analysis yielded clear clustering of two groups; dietary BS increased the relative abundance of Bacteroidales_unclassified (genus) and Olsenella (genus), and decreased the abundance of genera Alistipes on day 50, which identified a strong correlation with FCR, serum differential metabolites, or differential gene expression in the jejunal mucosa by spearman correlation analysis. The PICRUSt2 analysis revealed that supplementation with BS enriched the pathways related to xenobiotics biodegradation and metabolism, carbohydrate metabolism, energy metabolism, signaling molecules and interaction, the digestive system, and transport and catabolism. These results demonstrated that dietary BS increased feed efficiency, antioxidant capacity, and the mRNA expression of pro-inflammatory cytokines in the jejunal mucosa; and decreased the activity of diamine oxidase in serum, which might be attributed to the modulation of community composition and the functions of cecal microbiota in white-feathered broilers.

Structured grid-based sparse matrix-vector multiplication and Gauss–Seidel iterations are very important kernel functions in scientific and engineering computations, both of which are memory intensive and bandwidth-limited. GPDSP is a general purpose digital signal processor, which is a very significant embedded processor that has been introduced into high-performance computing. In this paper, we designed various optimization methods, which included a blocking method to improve data locality and increase memory access efficiency, a multicolor reordering method to develop Gauss–Seidel fine-grained parallelism, a data partitioning method designed for GPDSP memory structures, and a double buffering method to overlap computation and access memory on structured grid-based SpMV and Gauss–Seidel iterations for GPDSP. At last, we combined the above optimization methods to design a multicore vectorization algorithm. We tested the matrices generated with structured grids of different sizes on the GPDSP platform and obtained speedups of up to 41× and 47× compared to the unoptimized SpMV and Gauss–Seidel iterations, with maximum bandwidth efficiencies of 72% and 81%, respectively. The experiment results show that our algorithms could fully utilize the external memory bandwidth. We also implemented the commonly used mixed precision algorithm on the GPDSP and obtained speedups of 1.60× and 1.45× for the SpMV and Gauss–Seidel iterations, respectively.

Status epilepticus (SE), a condition with abnormally prolonged seizures, is a severe type of epilepsy. At present, SE is not well controlled by clinical treatments. Antiepileptic drugs (AEDs) are the main therapeutic approaches, but they are effective for SE only with a narrow intervening window, and they easily induce resistance. Thus, in this review, we provide an updated summary for an insight into drug-resistant SE, hoping to add to the understanding of the mechanism of refractory SE and the development of active compounds. Firstly, we briefly outline the limitations of current drug treatments for SE by summarizing the extensive experimental literature and clinical data through a search of the PubMed database, and then summarize the common animal models of refractory SE with their advantages and disadvantages. Notably, we also briefly review some of the hypotheses about drug resistance in SE that are well accepted in the field, and furthermore, put forward future perspectives for follow-up research on SE.

Urbanization brings great enrichment to human production and life, but also has certain environmental impact on the area where the city is located. Many studies have revealed the negative effects of urbanization on the ecological environment of urban or urban agglomerations, especially in the early stage of urbanization, but there are few studies on the impact on the peripheral ecological space environment. Will the peripheral environment be better off with less human interference as people move to cities during urbanization? In order to answer this question, we took the Guangdong-Hong Kong-Macao Greater Bay Area, the most economically dynamic area in China, as an example to explore the relationship between impervious changes of urban agglomerations monitored by remote sensing in the Bay Area and ecological indicators of forest and grassland in Guangdong Province outside the Bay area. The results showed that:(1) in the past 30 years, the area of grassland outside the bay area did not change regularly, while the area of forest decreased year by year. The landscape indices of forest and grassland were gradually fragmented and discrete. Moreover, the distribution of Fraction Vegetation Coverage (FVC) of forest and grassland has changed since before urbanization. (2) Through correlation analysis, it is found that the changes in forest area and the landscape index of forest and grassland are strongly correlated with the development of urbanization in the Greater Bay Area. This shows that the process of urbanization in the Greater Bay Area will have a non-negligible impact on the peripheral environment. In the process of urban development, we should not only focus on the inner city but also consider the outer environment of the city.

Over the last few years, cryo electron microscopy has become the most important method in structural biology. While 80% of deposited maps are from single particle analysis, electron tomography has grown to become the second most important method. In particular sub-tomogram averaging has matured as a method, delivering structures between 2 and 5 Å from complexes in cells as well as in vitro complexes. While this resolution range is not standard, novel developments point toward a promising future. Here, we provide a guide for the workflow from sample to structure to gain insight into this emerging field.

Measuring and testing association between categorical variables is one of the long-standing problems in multivariate statistics. In this paper, I define a broad class of association measures for categorical variables based on weighted Minkowski distance. The proposed framework subsumes some important measures including Cramér’s V, distance covariance, total variation distance and a slightly modified mean variance index. In addition, I establish the strong consistency of the defined measures for testing independence in two-way contingency tables, and derive the scaled forms of unweighted measures.