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20 results on '"Piccirilli, Alessandra"'

1. Synergistic Activity of Temocillin and Fosfomycin Combination against KPC-Producing Klebsiella pneumoniae Clinical Isolates

Author

Costantino, Venera, primary, Principe, Luigi, additional, Mehat, Jai, additional, Busetti, Marina, additional, Piccirilli, Alessandra, additional, Perilli, Mariagrazia, additional, Luzzati, Roberto, additional, Zerbato, Verena, additional, Meliadò, Antonietta, additional, La Ragione, Roberto, additional, and Di Bella, Stefano, additional

Published
2024
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2. Identification of IncA Plasmid, Harboring blaVIM-1 Gene, in S. enterica Goldcoast ST358 and C. freundii ST62 Isolated in a Hospitalized Patient

Author

Piccirilli, Alessandra, primary, Di Marcantonio, Sascia, additional, Costantino, Venera, additional, Simonetti, Omar, additional, Busetti, Marina, additional, Luzzati, Roberto, additional, Principe, Luigi, additional, Di Domenico, Marco, additional, Rinaldi, Antonio, additional, Cammà, Cesare, additional, and Perilli, Mariagrazia, additional

Published
2023
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3. New Antimicrobials for Gram-Positive Sustained Infections: A Comprehensive Guide for Clinicians

Author

Carcione, Davide, primary, Intra, Jari, additional, Andriani, Lilia, additional, Campanile, Floriana, additional, Gona, Floriana, additional, Carletti, Silvia, additional, Mancini, Nicasio, additional, Brigante, Gioconda, additional, Cattaneo, Dario, additional, Baldelli, Sara, additional, Chisari, Mattia, additional, Piccirilli, Alessandra, additional, Di Bella, Stefano, additional, and Principe, Luigi, additional

Published
2023
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5. Evaluation of the Analytical Performances of the Biolabo SOLEA 100 Optical Coagulometer and Comparison with the Stago STA-R MAX Analyser in the Determination of PT, APTT, and Fibrinogen

Author

Bellio, Pierangelo, primary, De Angelis, Simonetta, additional, Piccirilli, Alessandra, additional, Di Michele, Giulio, additional, Barnabei, Remo, additional, Amicosante, Gianfranco, additional, Perilli, Mariagrazia, additional, and Celenza, Giuseppe, additional

Published
2022
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7. Molecular Characterization by Whole-Genome Sequencing of Clinical and Environmental Serratia marcescens Strains Isolated during an Outbreak in a Neonatal Intensive Care Unit (NICU)

Author

Piccirilli, Alessandra, primary, Cherubini, Sabrina, additional, Brisdelli, Fabrizia, additional, Fazii, Paolo, additional, Stanziale, Andrea, additional, Di Valerio, Susanna, additional, Chiavaroli, Valentina, additional, Principe, Luigi, additional, and Perilli, Mariagrazia, additional

Published
2022
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8. In Vitro Activity of Sulbactam–Durlobactam against Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates: A Multicentre Report from Italy

Author

Segatore, Bernardetta, primary, Piccirilli, Alessandra, additional, Cherubini, Sabrina, additional, Principe, Luigi, additional, Alloggia, Giovanni, additional, Mezzatesta, Maria Lina, additional, Salmeri, Mario, additional, Di Bella, Stefano, additional, Migliavacca, Roberta, additional, Piazza, Aurora, additional, Meroni, Elisa, additional, Fazii, Paolo, additional, Visaggio, Daniela, additional, Visca, Paolo, additional, Cortazzo, Venere, additional, De Angelis, Giulia, additional, Pompilio, Arianna, additional, and Perilli, Mariagrazia, additional

Published
2022
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9. Exploiting Bacteria for Improving Hypoxemia of COVID-19 Patients

Author

Trinchieri, Vito, primary, Marazzato, Massimiliano, additional, Ceccarelli, Giancarlo, additional, Lombardi, Francesca, additional, Piccirilli, Alessandra, additional, Santinelli, Letizia, additional, Maddaloni, Luca, additional, Vassalini, Paolo, additional, Mastroianni, Claudio Maria, additional, and d’Ettorre, Gabriella, additional

Published
2022
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13. Oxygen Sparing Effect of Bacteriotherapy in COVID-19

Author

Ceccarelli, Giancarlo, primary, Marazzato, Massimiliano, additional, Celani, Luigi, additional, Lombardi, Francesca, additional, Piccirilli, Alessandra, additional, Mancone, Massimo, additional, Trinchieri, Vito, additional, Pugliese, Francesco, additional, Mastroianni, Claudio M., additional, and d’Ettorre, Gabriella, additional

Published
2021
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14. Biofilm Formation among Stenotrophomonas maltophilia Isolates Has Clinical Relevance: The ANSELM Prospective Multicenter Study

Author

Pompilio, Arianna, primary, Ranalli, Marco, additional, Piccirilli, Alessandra, additional, Perilli, Mariagrazia, additional, Vukovic, Dragana, additional, Savic, Branislava, additional, Krutova, Marcela, additional, Drevinek, Pavel, additional, Jonas, Daniel, additional, Fiscarelli, Ersilia V., additional, Tuccio Guarna Assanti, Vanessa, additional, Tavío, María M., additional, Artiles, Fernando, additional, and Di Bonaventura, Giovanni, additional

Published
2020
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15. Amino Acid Replacement at Position 228 Induces Fluctuation in the Ω-Loop of KPC-3 and Reduces the Affinity against Oxyimino Cephalosporins: Kinetic and Molecular Dynamics Studies

Author

Piccirilli, Alessandra, primary, Brisdelli, Fabrizia, additional, Docquier, Jean Denis, additional, Aschi, Massimiliano, additional, Cherubini, Sabrina, additional, De Luca, Filomena, additional, Matagne, André, additional, Amicosante, Gianfranco, additional, and Perilli, Mariagrazia, additional

Published
2020
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16. Whole-Genome Sequencing (WGS) of Carbapenem-Resistant K. pneumoniae Isolated in Long-Term Care Facilities in the Northern Italian Region

Author

Piccirilli, Alessandra, Cherubini, Sabrina, Azzini, Anna, Tacconelli, Evelina, Cascio, Giuliana Lo, Maccacaro, Laura, Bazaj, Alda, Naso, Laura, Amicosante, Gianfranco, Group, LTCF-Veneto Working, and Perilli, Mariagrazia

Subjects
Microbiology (medical), Whole genome sequencing, Genetics, Carbapenem resistant, QH301-705.5, Klebsiella pneumoniae, β lactamases, K pneumoniae, β-lactamases, biochemical phenomena, metabolism, and nutrition, Biology, bacterial infections and mycoses, biology.organism_classification, Microbiology, Virology, polycyclic compounds, Biology (General), WGS, Illumina dye sequencing
Abstract

K. pneumoniae (KPN) is one of the widest spread bacteria in which combined resistance to several antimicrobial groups is frequent. The most common β-lactamases found in K. pneumoniae are class A carbapenemases, both chromosomal-encoded (i.e., NMCA, IMI-1) and plasmid-encoded (i.e., GES-enzymes, IMI-2), VIM, IMP, NDM, OXA-48, and extended-spectrum β-lactamases (ESBLs) such as CTX-M enzymes. In the present study, a total of 68 carbapenem-resistant KPN were collected from twelve long-term care facilities (LTCFs) in the Northern Italian region. The whole-genome sequencing (WGS) of each KPN strain was determined using a MiSeq Illumina sequencing platform and analysed by a bacterial analysis pipeline (BAP) tool. The WGS analysis showed the prevalence of ST307, ST512, and ST37 as major lineages diffused among the twelve LTCFs. The other lineages found were: ST11, ST16, ST35, ST253, ST273, ST321, ST416, ST1519, ST2623, and ST3227. The blaKPC-2, blaKPC-3, blaKPC-9, blaSHV-11, blaSHV-28, blaCTX-M-15, blaOXA-1, blaOXA-9, blaOXA-23, qnrS1, qnrB19, qnrB66, aac(6′)-Ib-cr, and fosA were the resistance genes widespread in most LTCFs. In this study, we demonstrated the spreading of thirteen KPN lineages among the LTCFs. Additionally, KPC carbapenemases are the most widespread β-lactamase.

Published
2021

18. Acinetobacter baumannii Resistance to Sulbactam/Durlobactam: A Systematic Review

Author

Luigi Principe, Stefano Di Bella, Jacopo Conti, Mariagrazia Perilli, Alessandra Piccirilli, Cristina Mussini, Giuliana Decorti, Principe, Luigi, Di Bella, Stefano, Conti, Jacopo, Perilli, Mariagrazia, Piccirilli, Alessandra, Mussini, Cristina, and Decorti, Giuliana

Subjects
Microbiology (medical), Acinetobacter, efficacy, sulbactam/durlobactam, Biochemistry, Microbiology, susceptibility, sulbactam-durlobactam, resistance, Infectious Diseases, resistances, Pharmacology (medical), General Pharmacology, Toxicology and Pharmaceutics
Abstract

Infections caused by carbapenem-resistant Acinetobacter baumannii (CRAB) have limited therapeutic options. Sulbactam-durlobactam is a combination of two βlactamase inhibitors with activity against CRAB under phase 3 clinical investigation. We performed a systematic review on in vitro studies reporting A. baumannii resistances against sulbactam/durlobactam. We considered “resistant” species to be those with MIC ≥ 8 mg/L. Ten studies were included in the review (9754 tested isolates). Overall, 2.3% of A. baumannii were resistant to sulbactam/durlobactam, and this percentage rose to 3.4% among CRAB subgroups and to 3.7% among colistin-resistant strains. Resistance was 100% among metallo β-lactamase-producing strains. Overall, in 12.5% of cases, sulbactam/durlobactam resistance was associated with the production of NDM-1, in 31.7% of cases with the substitutions in the PBP3 determinants, and in the remaining cases the resistance mechanism was unknown. In conclusion, A. baumannii resistance towards sulbactam/durlobactam is limited, except for MBL-producing strains.

Published
2022

19. Identification of IncA Plasmid, Harboring bla VIM-1 Gene, in S. enterica Goldcoast ST358 and C. freundii ST62 Isolated in a Hospitalized Patient.

Author

Piccirilli A, Di Marcantonio S, Costantino V, Simonetti O, Busetti M, Luzzati R, Principe L, Di Domenico M, Rinaldi A, Cammà C, and Perilli M

Abstract

In the present study, we analyzed the genome of two S. enterica strains TS1 and TS2 from stool and blood cultures, respectively, and one strain of C. freundii TS3, isolated from a single hospitalized patient with acute myeloid leukemia. The S. enterica Goldcoast ST358 (O:8 (C2-C3) serogroup), sequenced by the MiSeq Illumina system, showed the presence of β-lactamase genes ( bla VIM-1 , bla SHV-12 and bla OXA-10 ), aadA1 , ant(2″)-Ia , aac(6')-Iaa , aac(6')-Ib3 , aac(6')-Ib-cr , qnrVC6 , parC(T57S), and several incompatibility plasmids. A wide variety of insertion sequences (ISs) and transposon elements were identified. In C. freundii TS3, these were the bla VIM-1 , bla CMY-150 , and bla SHV-12 , aadA1 , aac(6')-Ib3 , aac(6')-Ib-cr , mph(A) , sul1 , dfrA14 , ARR-2 , qnrVC6 , and qnrB38 . IncA plasmid isolated from E.coli /K12 transconjugant and C. freundii exhibited a sequence identity >99.9%. The transfer of IncA plasmid was evaluated by conjugation experiments.

Published
2023
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20. Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region.

Author

Cherubini S, Perilli M, Azzini AM, Tacconelli E, Maccacaro L, Bazaj A, Naso L, Amicosante G, Ltcf-Veneto Working Group, Lo Cascio G, and Piccirilli A

Abstract

Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of view, 43 E. coli strains collected from residents of LTCFs in Northern Italy. The most common lineage found was ST131, followed by sporadic presence of ST12, ST69, ST48, ST95, ST410 and ST1193. All strains were incubators of several virulence factors, with iss , sat , iha and senB being found in 84%, 72%, 63% and 51% of E. coli , respectively. Thirty of the ST131 analyzed were of the O25b:H4 serotype and H30 subclone. The ST131 isolates were found to be mainly associated with IncF plasmids, CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-27 and gyrA/parC/parE mutations. Metallo-β-lactamases were not found in ST131, whereas KPC-3 carbapenemase was found only in two ST131 and one ST1193. In conclusion, we confirmed the spread of extended-spectrum β-lactamase genes in E. coli ST131 isolated from colonized residents living inside LTCFs. The ST131 represents an incubator of fluoroquinolones, aminoglycosides and other antibiotic resistance genes in addition to different virulence factors.

Published
2022
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