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4 results on '"Nur Mahiza Md Isa"'

1. High Granulocyte-Macrophage Colony Stimulating Factor to Interleukin 10 Ratio and Marked Antioxidant Enzyme Activities Predominate in Symptomatic Cattle Naturally Infected with Candidatus Mycoplasma haemobos, Theileria orientalis, Theileria sinensis and Trypanosoma evansi

Author

Onyinyechukwu Ada Agina, Kim Tho Cheah, Nurul Syahirah Ahmad Sayuti, Mohd Rosly Shaari, Nur Mahiza Md Isa, Mokrish Ajat, Mohd Zamri-Saad, Mazlina Mazlan, and Hazilawati Hamzah

Subjects
cytokines, serum, ELISA, asymptomatic and symptomatic cattle, blood parasites, oxidant/antioxidant, Veterinary medicine, SF600-1100, Zoology, QL1-991
Abstract

The aim of this study was to measure the serum proinflammatory (IL-12, GM-CSF & IFN-γ) to anti-inflammatory (IL-10, IL-4) cytokine ratio, oxidant (MDA) level and antioxidant enzyme (SOD; GPx) activities after blood parasite infections. The blood and serum samples were obtained from 130 cattle and screened for identity of the infecting blood parasites by conventional PCR. The following blood parasite species were detected: Candidatus Mycoplasma haemobos (70/130); Theileria orientalis (65/130); Theileria sinensis (32/130); Anaplasma marginale (49/130); Anaplasma platys (7/130); and Trypanosoma evansi (4/130). The GM-CSF/IL-10 ratio showed significantly higher values in all the symptomatic blood parasite infected cattle groups except for symptomatic A. platys infected cattle groups. Anti-inflammatory cytokine immune responses were notable findings in symptomatic and asymptomatic cattle infected with C. M. haemobos and T. orientalis characterized by low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 (p < 0.05). Therefore, high serum GM-CSF:IL:10 in the symptomatic blood parasite infected cattle, low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 ratios in asymptomatic cattle, high MDA level, and increased antioxidant enzyme activities could be useful predictive tools for outcome of natural blood parasite infections in cattle.

Published
2021
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2. Seroprevalence and Risk Factors of Toxoplasma gondii in Ruminant Meats from Wet Markets in Klang Valley and Abattoirs in Selangor, Malaysia

Author

Norhamizah Abdul Hamid, Mohammed Babatunde Sadiq, Siti Zubaidah Ramanoon, Rozaihan Mansor, Malaika Watanabe, Nur Mahiza Md Isa, Juriah Kamaludeen, and Sharifah Salmah Syed-Hussain

Subjects
Toxoplasma gondii, ruminants, wet markets, abattoir, meat juice, ELISA, Veterinary medicine, SF600-1100, Zoology, QL1-991
Abstract

(1) Background: The objective of this study was to determine the prevalence of T. gondii in meats of cattle, goat and sheep from wet markets in Klang Valley, and abattoirs in Selangor, Malaysia; (2) Methods: A total of 192 meat samples were purchased from 51 wet markets in six districts in Klang Valley (Gombak, Klang, Kuala Lumpur, Hulu Langat, Petaling and Putrajaya). Meanwhile, a total of 200 diaphragm samples were collected from two government abattoirs located in Shah Alam and Banting, Selangor. All meat juices from samples were subjected to an indirect-ELISA kit for the presence of T. gondii IgG antibodies. Furthermore, all 184 meat samples of goat and sheep were subjected to conventional nested PCR (B1 genes) for the detection of T. gondii DNA; (3) Results: T. gondii antibodies were detected in 25% (n = 98/392) of the samples with seroprevalence of 9.1% (19/208, CI: 5.9%–13.8%) in cattle meat; 54.7% (41/75, 95% CI: 43.5%–65.4%) in goat meat and 34.9% (38/109, CI: 26.6%–44.2%) in sheep meat. No T. gondii DNA was detected in any of the meat samples of goat and sheep. T. gondii seropositivity in wet market samples was higher in goat (OR = 37.1 CI 12.4–110.3) and sheep meat (OR 9.03 CI: 3.28–24.8) compared to cattle meat (OR = 1.0) At univariate level, meat from non-licensed abattoirs (OR = 6.0 CI: 2.9–12.3) and female animals (OR = 6.7; CI 1.9–22.6) had higher risks of being seropositive for T. gondii antibodies than licensed abattoirs and male animals, respectively. (4) Conclusions: This is the first report of seroprevalence of T. gondii in ruminant meats for human consumption in Malaysia. The findings signified high exposure of meat samples from wet markets to T. gondii and the need for control measures to reduce the likelihood of infection when such raw or undercooked meats are consumed.

Published
2020
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3. High Granulocyte-Macrophage Colony Stimulating Factor to Interleukin 10 Ratio and Marked Antioxidant Enzyme Activities Predominate in Symptomatic Cattle Naturally Infected with Candidatus Mycoplasma haemobos, Theileria orientalis, Theileria sinensis and Trypanosoma evansi

Author

Mazlina Mazlan, Nur Mahiza Md Isa, Mohd Zamri-Saad, Nurul Syahirah Ahmad Sayuti, Mohd Rosly Shaari, Kim Tho Cheah, Onyinyechukwu Ada Agina, Mokrish Ajat, and Hazilawati Hamzah

Subjects
0301 basic medicine, Anaplasma platys, 040301 veterinary sciences, medicine.medical_treatment, Veterinary medicine, asymptomatic and symptomatic cattle, Asymptomatic, Article, Proinflammatory cytokine, Microbiology, 0403 veterinary science, 03 medical and health sciences, Immune system, SF600-1100, medicine, General Veterinary, biology, 04 agricultural and veterinary sciences, Trypanosoma evansi, biology.organism_classification, cytokines, Interleukin 10, 030104 developmental biology, Cytokine, Granulocyte macrophage colony-stimulating factor, blood parasites, QL1-991, oxidant/antioxidant, Animal Science and Zoology, ELISA, medicine.symptom, serum, Zoology, medicine.drug
Abstract

The aim of this study was to measure the serum proinflammatory (IL-12, GM-CSF &amp, IFN-γ) to anti-inflammatory (IL-10, IL-4) cytokine ratio, oxidant (MDA) level and antioxidant enzyme (SOD, GPx) activities after blood parasite infections. The blood and serum samples were obtained from 130 cattle and screened for identity of the infecting blood parasites by conventional PCR. The following blood parasite species were detected: Candidatus Mycoplasma haemobos (70/130), Theileria orientalis (65/130), Theileria sinensis (32/130), Anaplasma marginale (49/130), Anaplasma platys (7/130), and Trypanosoma evansi (4/130). The GM-CSF/IL-10 ratio showed significantly higher values in all the symptomatic blood parasite infected cattle groups except for symptomatic A. platys infected cattle groups. Anti-inflammatory cytokine immune responses were notable findings in symptomatic and asymptomatic cattle infected with C. M. haemobos and T. orientalis characterized by low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 (p &lt, 0.05). Therefore, high serum GM-CSF:IL:10 in the symptomatic blood parasite infected cattle, low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 ratios in asymptomatic cattle, high MDA level, and increased antioxidant enzyme activities could be useful predictive tools for outcome of natural blood parasite infections in cattle.

Published
2021

4. Seroprevalence and Risk Factors of Toxoplasma gondii in Ruminant Meats from Wet Markets in Klang Valley and Abattoirs in Selangor, Malaysia

Author

Juriah Kamaludeen, Nur Mahiza Md Isa, Siti Zubaidah Ramanoon, Malaika Watanabe, S.S. Syed-Hussain, M.B. Sadiq, Norhamizah Abdul Hamid, and Rozaihan Mansor

Subjects
Veterinary medicine, 040301 veterinary sciences, abattoir, 030231 tropical medicine, Meat juice, Toxoplasma gondii, 0403 veterinary science, 03 medical and health sciences, 0302 clinical medicine, fluids and secretions, Ruminant, parasitic diseases, lcsh:Zoology, Seroprevalence, meat juice, lcsh:QL1-991, Kuala lumpur, lcsh:Veterinary medicine, General Veterinary, biology, technology, industry, and agriculture, food and beverages, 04 agricultural and veterinary sciences, biology.organism_classification, ruminants, wet markets, lcsh:SF600-1100, Animal Science and Zoology, ELISA, Nested polymerase chain reaction, human activities
Abstract

(1) Background: The objective of this study was to determine the prevalence of T. gondii in meats of cattle, goat and sheep from wet markets in Klang Valley, and abattoirs in Selangor, Malaysia, (2) Methods: A total of 192 meat samples were purchased from 51 wet markets in six districts in Klang Valley (Gombak, Klang, Kuala Lumpur, Hulu Langat, Petaling and Putrajaya). Meanwhile, a total of 200 diaphragm samples were collected from two government abattoirs located in Shah Alam and Banting, Selangor. All meat juices from samples were subjected to an indirect-ELISA kit for the presence of T. gondii IgG antibodies. Furthermore, all 184 meat samples of goat and sheep were subjected to conventional nested PCR (B1 genes) for the detection of T. gondii DNA, (3) Results: T. gondii antibodies were detected in 25% (n = 98/392) of the samples with seroprevalence of 9.1% (19/208, CI: 5.9%&ndash, 13.8%) in cattle meat, 54.7% (41/75, 95% CI: 43.5%&ndash, 65.4%) in goat meat and 34.9% (38/109, CI: 26.6%&ndash, 44.2%) in sheep meat. No T. gondii DNA was detected in any of the meat samples of goat and sheep. T. gondii seropositivity in wet market samples was higher in goat (OR = 37.1 CI 12.4&ndash, 110.3) and sheep meat (OR 9.03 CI: 3.28&ndash, 24.8) compared to cattle meat (OR = 1.0) At univariate level, meat from non-licensed abattoirs (OR = 6.0 CI: 2.9&ndash, 12.3) and female animals (OR = 6.7, CI 1.9&ndash, 22.6) had higher risks of being seropositive for T. gondii antibodies than licensed abattoirs and male animals, respectively. (4) Conclusions: This is the first report of seroprevalence of T. gondii in ruminant meats for human consumption in Malaysia. The findings signified high exposure of meat samples from wet markets to T. gondii and the need for control measures to reduce the likelihood of infection when such raw or undercooked meats are consumed.

Published
2020

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