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The aim was to objectively assess compliance in patients prescribed headgear and evaluate the impact of monitoring awareness, treatment duration, gender, and age on compliance levels. A total of 22 patients with Class II malocclusion wore the headgear integrated with the force and temperature sensitive Smartgear monitoring system (Smartgear, Swissorthodontics AG, Cham, Switzerland). Patients were instructed to wear the headgear for 13 h daily over a 3-month period. Randomly, 11 patients were informed that they monitored and 11 were not informed. Data were organized using Microsoft Excel and analyzed using R for statistical estimates, graphs, and hypothesis testing. Smartgear recorded an average daily compliance of 6.7 h. No statistically significant differences were found in cooperation between study group and control group over the 3 months of treatment, regardless of gender and age. However, there was slight greater cooperation in the first month than in the other months, and patients ≤10 years of age had almost 2 h more cooperation than their older counterparts. Moreover, the informed group exhibited an average of 1.1 more hours of cooperation per day than the uninformed group, which may carry clinical significance. This cooperation primarily occurred at night and was found to be statistically significant. Compliance among young patients typically remained lower than the prescribed level, regardless of their gender and psychological maturity. Although an awareness of monitoring does not seem to improve compliance, implementing such systems could still offer dentists a valuable means of obtaining objective information about their patients’ adherence.

Objective: To evaluate the differences in frictional resistance between in-house 3D-printed resin brackets (IH3DBs) and two types of commercially available brackets in combination with three different archwires. Methods: Friction tests were performed using a dynamometer and a millimetre sled to simulate first premolar post-extraction space closure. Three different brackets, namely PRIMO metallic brackets, Crystal composite brackets and in-house 3D-printed brackets, were tested in combination with three different archwires (0.016-inch NiTi, 0.019 × 0.025-inch NiTi and 0.019 × 0.025-inch SS). Statistical analysis was performed to verify the differences in friction among the bracket and archwire combinations. For all the tests, the significance level was set at p < 0.05. Results: There were significant differences among the three brackets tested with both the 0.016-inch NiTi and 0.019 × 0.025-inch SS archwires (p = 0.026 and p = 0.017, respectively). Only tests with the 0.019 × 0.025-inch NiTi archwire yielded no statistically significant differences between the groups. The composite bracket generated clinically and statistically more friction than both the IH3DB and metallic bracket, with no differences between the latter two. Conclusions: The IH3DBs demonstrated comparable frictional resistance properties to the metal brackets and better than the composite brackets with all the archwires tested.

Background: To evaluate colour stability in artificial saliva by mechanically simulating brushing between in-house 3D-printed resin brackets (IH3DBs) and commercially available brackets. Methods: The samples consist of four sets of ten aesthetic brackets each supplied by four different manufacturers: clear Damon, Synovate C, Crystal and in-house 3D printed brackets (IH3DBs). The brackets were immersed in a plastic tank containing artificial saliva maintained at a constant temperature of 37 °C with a 65-minute brushing system. Staining changes at T0 (pre-brushing) and T1 (post-brushing) were measured with a spectrophotometer according to the VITA and Master scale and brightness values. Statistical analysis compared the colour changes with the Wilcoxon test and in case of significance, severity was investigated. The significance level considered was p < 0.05. Results: The IH3DBs shows statistically significant differences for both scales and brightness values. The Damon and the Crystal brackets report a statistically significant difference only for brightness. The Synovate C bracket shows no statistically significant differences. Conclusions: The IH3DB produced chromaticity differences for the VITA and Master scale possibly due to the surface roughness created during the printing process. However, the IH3DBs together with Damon Clear improved brightness, due to the mechanical action of brushing.

The following prospective study has the aim of evaluating the efficiency of the F22 MAD (mandibular advancement device), a new oral device for the treatment of OSAS (Obstructive Sleep Apnea Syndrome) and snoring. Methods: AHI (apnea-hypopnea index), ODI (Oxygen Desaturation Index), snoring percentage, time spent in the supine position, and Epworth Sleepiness Scale score were evaluated in 19 patients with snoring, mild to moderate OSAS, or severe OSAS who declined CPAP (Continuous Positive Air Pressure) treatment, before and after the application of the F22 MAD. Results: The median value of AHI varied from 15.6 ± 10.7 to 5.7 ± 5.7; the median value of ODI varied from 13.4 ± 8.8 to 6.2 ± 5.2; the median value of the percentage of snoring varied from 30.7 ± 7 to 7.5 ± 10.8, except for the patient who has severe OSAS who increased their value. The value obtained by the self-completion of the ESS questionnaire (Epworth Sleepiness Scale) underwent a statistically significant variation, while clinically significant for 13\19 patients who obtained a reduction of the value >/= of 2 points. Conclusions: It is possible to conclude that the F22 MAD is effective in the treatment of patients with mild and moderate OSAS or simple snoring, reducing the polysomnographic outcomes with statistically and clinically significant results in terms of reduction of AHI, ODI and percentage of snoring.

This retrospective study aims to further investigate the effects of rapid maxillary expansion (RME) treatment on respiratory function in growing patients with contracted upper airways, by assessing nasal resistance using active anterior rhinomanometry (AAR). Methods: At T0 (pre-RME), 122 orthodontic patients aged 5 to 12 years, exhibiting oral breathing and/or snoring along with maxillary contraction, underwent AAR with a mask using the ATMOS Rhino 31 rhinomanometer. Additionally, the SRDB or PSQ-SRDB Questionnaire was utilized to validate the orthodontic history of obstructive sleep apnea syndrome (OSAS). If AAR results at T0 were clinically significant, they were repeated at T1, corresponding to a period of three months after screw fixation, to evaluate changes in nasal resistance values between pre- and post-treatment. Results: The study group comprised 42 oral respiratory patients with a mean age of 7.71 years, suspected of pediatric OSAS. Although the questionnaires did not yield statistically significant results, the therapy induced an overall reduction in airflow resistance values from both nostrils (−24.63% in the right nostril; −26.65% in the left nostril). Conclusions: This study demonstrates the beneficial effects of orthodontic treatment with RME on nasal function in terms of airflow resistance in cases of maxillary contraction.

Digitalization techniques, such as photogrammetry (PG), are attracting the interest of experts in the cultural heritage field, as they enable the creation of three-dimensional virtual replicas of historical artifacts with 2D digital images. Indeed, PG allows for acquiring data regarding the overall appearance of an artifact, its geometry, and its texture. Furthermore, among several image-based techniques exploited for the conservation of works of art, multispectral imaging (MSI) finds great application in the study of the materials of historical items, taking advantage of the different responses of materials when exposed to specific wavelengths. Despite their great usefulness, PG and MSI are often used as separate tools. Integrating radiometric and geometrical data can notably expand the information carried by a 3D model. Therefore, this paper presents a novel research methodology that enables the acquisition of multispectral 3D models, combining the outcomes of PG and MSI (Visible (VIS), Ultraviolet-induced Visible Luminescence (UVL), Ultraviolet-Reflected (UVR), and Ultraviolet-Reflected False Color (UVR-FC) imaging) in a single coordinate system, using an affordable tunable set-up and open-source software. The approach has been employed for the study of two wooden artifacts from the Museo Egizio di Torino to investigate the materials present on the surface and provide information that could support the design of suitable conservation treatments.

Background: To investigate the accuracy of an F22 Aligner system, considering the amount of prescribed movement, tooth type, grip points, sex and age. Methods: Digital models of 120 patients (64 females and 56 males; mean age 35.2 years ± 7.4) affected by mild-to-moderate Class I malocclusion and treated via F22 Aligners, retrospectively selected from the University of Ferrara Orthodontics Clinic’s electronic database; post-treatment models were generated, and three angular values per tooth and four linear intra-arch measurements per arch were acquired. For angular measurements, planned (T1) and achieved (T2) values were obtained thorough digital model superimpositions. Linear measurements were acquired from pre-treatment, reference and post-treatment models. Statistical comparisons were performed to assess accuracy among tooth types and prescribed movements, tooth type, grip points, sex and age were investigated via chi-squared automatic interaction detection regression trees. Results: Mean accuracy for inclination and angulation were 86.76% and 88.01%, respectively, whereas rotation was less accurate (61.59%), especially for rounded teeth. All variables investigated influenced accuracy, with the exception of inclination, which was only influenced by age. Regarding linear measurements, good expansive capacity was shown, except for the distance between mandibular second premolars. Conclusions: F22 aligners are a viable solution for the treatment of Class I malocclusion of mild-to-moderate complexity, although clinicians should bear in mind the lower predictability of rotation, as well as the influence of the variables investigated.

(1) Background: Since 2013, the pathogenic bacterium Xylella fastidiosa has been severely affecting olive production in Apulia, Italy, with consequences for the economy, local culture, landscape and biodiversity. The production of a phenolic extract from fresh olive leaves was employed for endotherapeutic injection into naturally infected olive trees by Xylella fastidiosa in Apulia region, Italy. (2) Methods: The effectiveness of the extract was tested in vitro and in planta in comparison with analogous treatments based on garlic powder and potassium phosphite. (3) Results: The uptake of phenolic compounds from olive leaves through a trunk injection system device resulted in a statistically significant increase in leaf area index and leaf area density, as well as in the growth of newly formed healthy shoots. Plant growth-promoting effects were also observed for potassium phosphite. Moreover, the bacteriostatic activities of the phenolic extract and of the garlic-powder-based solution have been demonstrated in in vitro tests. (4) Conclusions: The results obtained and the contained costs of extraction make the endotherapeutic treatment with phenolic compounds a promising strategy for controlling X fastidiosa to be tested on a larger scale, although the experiments conducted in this study proved not to be suitable for centenary trees.

The aim of this paper is to perform an observational descriptive study of the Obstructive Sleep Apnea Syndrome (OSAS) in a population of children by evaluating the prevalence and role of sex and age variables. The 22-item Pediatric Sleep Questionnaire (PSQ) was administered to parents of children aged 3 to 12 years. The questionnaire is a very simple tool since it allows for the indication of patients with possible OSAS diagnosis through a cut-off of 0.33. The anonymous diagnostic questionnaire, available in digital format, was administered to the population under study by a link or QR code. Only the questionnaires completed in all their parts were recorded and analyzed. Eight hundred and thirty-two questionnaires were collected. One hundred and fifty-four subjects obtained a PSQ score > 0.33 and the prevalence of OSAS was 18.51%. The Chi-square test showed a statistically significant association between the PSQ score > 0.33 and male sex. The higher prevalence of subjects with a value of PSQ > 0.33 (n = 277) are aged 3–4–5 years, followed by the 6–7–8 range in the group with PSQ score > 0.33, p < 0.05. The prevalence of OSAS was 18.51% in children aged 3 to 12 years. The variables male biological sex and the age group 3–8 year were statistically significant for subjects with OSAS diagnosis. This study underlines the use of the 22-item Pediatric Sleep Questionnaire as a first screening tool to identify children at risk of OSAS.

The presence and infestation level of Bactrocera oleae was monitored in an organic olive orchard divided into differently treated parcels with kaolin (K), spinosad (S) and with kaolin and spinosad (K + S) in alternate rows. The treatments did not seem to affect olive fruit fly population dynamics, while statistically significant protective effects were recorded against total and harmful infestation, but not against the active one. Eventually, neither kaolin nor spinosad were shown to have a particular detrimental effect against naturally occurring B. oleae parasitoids.

(1) Background: the aim of this study was to investigate the transmittance levels of aligners in relation to different thermoforming disks and printers after 14 days of in vitro aging. (2) Methods: the transmittance level of 18 aligners was investigated. Three printers were used to create the aligner: Carbon L1, Prodways LD20 and RapidShape D100+. Each printer produced 6 aligners: three were made of Zendura FLX material and three of Scheu Ca Pro+. Each sample was subjected to spectrophotometry analysis of its transmittance levels, for a total of 54 measurements at T0. Then, all samples were aged in vitro at a constant temperature in artificial saliva supplemented with food coloring for 14 days each. The spectrophotometry protocol was repeated, and the same 54 measurements were collected at T1 (after aging). The resulting data were analyzed and compared by means of ANOVA (p < 0.05). (3) Results: all tested aligners revealed lower transmittance values after aging. Scheu CA Pro+ aligners showed higher transparency at T0, but a significant worsening of its aesthetic at T1. On the other hand, Zendura FLX aligners presented slightly reduced transmittance levels before aging, which, however, remained more stable after 14 days, showing no significant difference. In the specific, aligners produced by Prodways LD20 printers showed better optical properties than the others, both at T0 and T1. (4) Conclusions: Differences in pre-and-post-aging optical properties of aligners are influenced by the manufacturing process. Both the thermoplastic disks and the printers, thanks to new technologies, played a positive role in the final transparency of the product, which improved in all values compared to previous studies.

Aims: The purpose of this study was to evaluate the accuracy of the slot height of in-house 3D-printed resin brackets, comparing them with other types of brackets on the market today, both ceramic and metallic. Methods: Seven different types of bracket systems were selected. For each system, ten brackets for tooth 2.1 with 0.022 × 0.028-inch slots were selected (total n° 70). Considering the whole sample, five types were commercially available and two were in-house 3D-printed. The entire sample was divided into four different groups according to the bracket material and the method of holding the archwire. Precision pin gauges with 0.002-mm increments were inserted inside the slot of each bracket, and the slot heights were measured, microscopically ensuring that the gauge completely filled the slot, with full contact between both the bottom and the top of the slot. Results: With respect to the other five types of brackets on the market, the two types of in-house 3D-printed resin brackets showed great accuracy of slot height (0.558 ± 0.001 mm). There was a statistically significant difference between the real height measured and the nominal height declared by the manufacturers (p < 0.05) of all the samples investigated, with the exception of in-house 3D-printed resin brackets. Furthermore, the difference in slot height accuracy between commercially manufactured and in-house 3D-printed resin brackets was statistically significant. Conclusions: In-house 3D-printed resin brackets have a remarkably precise slot height, unlike commercially available brackets, whose slot heights tend to be significantly oversized with respect to the nominal values declared by the manufacturers.

We aimed to investigate the stress relaxation properties of five different thermoplastic aligner materials subjected to 14 days of constant deflection. Five different thermoplastic aligner materials were selected, whose elastic properties varied: F22 Evoflex, F22 Aligner, Durasoft, Erkoloc-Pro and Duran. The static properties of these materials—in particular, stiffness, stress–strain curve and yield stress—were measured with a three-point bending test. For all the tests that were performed, a minimum of three samples per material were tested. The yield load, yield strength, deformation and particularly the stiffness of each material were found to be similar in the single-layer samples, while the double-layer samples showed far lower stiffness values and were similar one to another. F22 Evoflex and Erkoloc-Pro maintained the highest percentages of stress, 39.2% and 36.9%, respectively, during the 15-day period. Duran and Durasoft obtained the lowest final stress values, 0.5 MPa and 0.4 MPa, respectively, and the lowest percentage of normalized stress, 4.6% and 3.9%, respectively, during the 15-day period. All the materials that we tested showed a rapidity of stress decay during the first few hours of application, before reaching a plateau phase. The F22 Evoflex material showed the greatest level of final stress, with relatively constant stress release during the entire 15-day period. Further research after in vivo aging is necessary in order to study the real aligners’ behavior during orthodontic treatment.

Objective: The purpose of this retrospective preliminary study was to analyze the skeletal and dentoalveolar effects of a new clear functional appliance in a sample of patients close to growth spurt with class II malocclusion attributable to mandibular retrusion. Materials and methods: A sample of growing patients underwent functional Class II correction using F22® (Sweden and Martina, Due Carrare (PD), Italy) Young, realized combining the aesthetic characteristics of the F22® clear aligner with the structural features of a functional appliance. For each patient, a digital setup was performed by a single operator to plan a mandibular advancement to the therapeutic position. Cephalometric analysis before and after treatment was performed. The purpose of the statistical analysis was to evaluate dental and skeletal changes associated with F22® Young device, and whether there were statistically significant differences in anatomical measurements between the beginning and end of treatment. Results: 15 patients, 7 females and 8 males of average age 10.3, were treated with the F22® Young appliance for an average period of 10 months ± 0.5 (maximum 11.5 months, minimum 10.5 months). The data showed that the SNB angle increase was statistically significant from T0 to T1, leading to a reduction in the ANB angle. Values for the mandible length, both total (Co-Gn) and at the base (Go-Pg), significantly increased. Conclusions: According to this preliminary study, F22® Young appliance is effective in promoting mandibular advancement when used near the pubertal growth peak. The device is comfortable and aesthetic, which certainly promoted patient compliance, a key factor for treatment success.

Olive (Olea europaea L.) is an evergreen xerophytic tree characterizing vegetative landscape and historical-cultural identity of the Mediterranean Basin. More than 2600 cultivars constitute the rich genetic patrimony of the species cultivated in approximately 60 countries. As a subtropical species, the olive tree is quite sensitive to low temperatures, and air temperature is the most critical environmental factor limiting olive tree growth and production. In this present review, we explored the detrimental effects caused of low temperatures on olive cultivars, and analyzed the most frequently experimental procedures used to evaluate cold stress. Then, current findings freezing stress physiology and gene are summarized in olive tree, with an emphasis on adaptive mechanisms for cold tolerance. This review might clear the way for new research on adaptive mechanisms for cold acclimation and for improvement of olive growing management.

The aim of the present study is to represent the orthodontic treatment of a young patient with a skeletal Class III malocclusion(Wits Appraisal −9 mm), in which the extractions of the first lower premolars were performed to obtain a class III camouflage using direct temporary anchorage devices (TADs). The patient reported a history of three years of orthodontic treatment at another clinic and the radiographic evaluation revealed an important impairment of the upper root incisors. A treatment was performed with a fixed appliance in the upper and lower arches using an archwire sequence of 0.16 CuNiTi, 19 × 25 CuNiTi, 19 × 25 SS and was carried out in 18 months. Post-treatment records of our patient show a control of facial esthetics from the frontal and lateral perspectives, with a harmonious soft-tissue profile. A Class I canine was achieved and her overbite and overjet were normalized by retracting the mandibular anterior teeth and carrying out a bodily mesialization of the posterior sector into the extraction space. In conclusion, the “pseudoankylosis system” used in this case allowed the desired result to be achieved with minimal change to the lower incisal inclination and without overloading the anterior upper and lower sectors, thus reducing the risk of further root resorption and patient compliance.

Xylella fastidiosa subsp. pauca, strain CoDiRO is the bacterium responsible for the onset of the disease known as the olive quick decline syndrome, which has been causing a phytosanitary and economic emergency in the Apulia region since 2013. To date, three insect species have been identified as pathogenic carriers of X. fastidiosa. With the advancement of the infection front, and the possibility of pathogenic insects being “hitchhiked” over long distances, the monitoring of the vectors of X. fastidiosa in the Italian regions bordering Apulia is an increasingly contingent issue for the rapid containment of the bacterium and the protection of the olive-growing heritage. Accordingly, the present research concerned the capture and recognition of the vector insects of X. fastidiosa in the upper Ionian coasts of Calabria (Italy) to evaluate the possible presence of the bacterium through molecular diagnostic techniques. The sampling allowed us to ascertain the presence of Philaenus spumarius and Neophilaenus campestris and their preferential distribution in olive groves and meadows, whereas all the 563 individuals tested negative for the pathogen.

The Italian extra virgin olive oil supply chain has considerable potential for embarking on a path of sustainable development and evolution. In Italy, the great variety heritage and the different pedo-climatic characteristics result in local olive growing systems with different management techniques, producing extra virgin olive oils that are strictly entwined to the territory, with peculiar qualitative properties. Nevertheless, numerous criticalities have been traditionally eroding the competitiveness of Italian olive growing that could find in sustainability certifications, a lasting driver of value creation. Shared standardizations and certifications that include the three pillars of sustainability are therefore necessary for the development of the process.

Objectives: This study aimed to assess frequency and multiple-night variability of sleep bruxism (SB) as well as sleep-time masticatory muscle activities (sMMA) in the home environment in healthy young adults using a portable device that provides electrocardiographic (ECG) and surface electromyographic (EMG) recordings from the masticatory muscles. Methods: The study was performed on 27 subjects (11 males, 16 females; mean age 28.3 ± 1.7 years) selected from a sample of healthy young students. Evaluation was carried out for four nights to record data on masticatory muscle activities using a compact portable device that previously showed an excellent agreement with polysomnography (PSG) for the detection of SB events. The number of SB episodes per sleep hour (bruxism index), and the number of tonic, phasic and mixed sMMA events per hour were assessed. A descriptive evaluation of the frequency of each condition was performed on all individuals, and gender comparison was investigated. Results: Mean sleep duration over the four recording nights was 7 ± 1.3 h. The average SB index was 3.6 ± 1.2. Most of the sMMA were tonic (49.9%) and phasic (44.1%). An ANOVA test showed the absence of significant differences between the four nights. No significant gender differences were detected for the SB index, phasic or tonic contractions; conversely, gender differences were detected for mixed sMMA events (p < 0.05). Conclusion: This investigation supports the concept that sMMA events are quite frequent in healthy adults. Differences over the four-night recording span were not significant. These data could be compared to subjects with underlying conditions that may lead to an additive bruxism activity and possible clinical consequences.

This work was designed to investigate the effect of mechanical tillage on glomalin content, arbuscular mycorrhizal fungi (AMF) abundance and diversity, and the concentration of water stable aggregates (WSA), in two adjacent olive groves located in Basilicata (Italy) that were managed over the course of 11 years in accordance with different horticultural models (conventional and sustainable). Soil sampling was performed at four depths between the trees within a row and between rows. In the end, WSA was found to be a highly sensitive indicator (especially in the “macro” fraction) of the effect of management on soil structure, showing the highest statistically significant values within the sustainable system. In the same regard, the diversity of the AM fungal community was negatively affected by conventional practices; on the other hand, a higher concentration of glomalin in the first 20 cm layer of the conventional system is here reported for the first time, as a likely result of disruption of the mycelium provoked by the mechanical tillage.

The primary impetus behind this research was to provide a boost to the characterization of the Italian olive biodiversity by acquiring reliable and homogeneous data over the course of an eight-year trial on the floral and fruiting biology of 120 molecularly analyzed cultivars, most of which have either low or very low diffusion. The obtained data highlighted a considerable variability to almost all of the analyzed parameters, which given the uniformity of environment and crop management was indicative of a large genetic variability in the accessions under observation, as confirmed through the molecular analysis. Several cases of synonymy were reported for the first time, even among plants cultivated in different regions, whilst all of the varieties examined, with only one exception, showed very low percentages of self-fruit-set, indicating a need for the employment of suitable pollinator plants. Eventually, a fitted model allowed us to evaluate the clear effects of the thermal values on blossoming, particularly in the months of March and April, whereas the climatic conditions during the flowering time had only a modest effect on its duration.

The analysis of forces, moments and pressure points has long been of great interest in orthodontics. Hence, we set out to define a method for measuring the pressure exerted by aligners on the teeth, and specifically to identify the precise points of pressure exertion. Intraoral scans were performed on a patient with optimal alignment and levelling before and after 2º vestibularisation of the upper central incisor. Pressure sensor film was placed in a dedicated housing between the aligner and teeth in order to record the pressure exerted after 15 s of aligner application. The images captured by the film were scanned, digitised, and subsequently analysed. Areas and amounts of pressure generated by the aligners were evaluated, and the net force of each was calculated, adjusted to take into consideration passive values. The method revealed the areas of contact by which the aligner transmits force on the teeth, and the pressures at which it does so. The pressure exerted by an aligner is not evenly distributed across the entire surface of the tooth during lingual tipping of an upper incisor. The areas of force concentration were not identical, as these are influenced by factors resulting from the manufacturing and casting processes.

The development of an electrochemical dissolved oxygen (DO) sensor based on a novel Cu(II) complex-modified screen printed carbon electrode is reported. The voltammetric behavior of the modified electrode was investigated at different scan rates and oxygen concentrations in PBS (pH = 7). An increase of cathodic current (at about −0.4 vs. Ag/AgCl) with the addition of oxygen was observed. The modified Cu(II) complex electrode was demonstrated for the determination of DO in water using chronoamperometry. A small size and low power consumption home-made portable electrochemical analyzer based on custom electronics for sensor interfacing and operating in voltammetry and amperometry modes has been also designed and fabricated. Its performances in the monitoring of DO in water were compared with a commercial one.