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3 results on '"Laura, Voigt"'

1. Thermophysical Properties of FUNaK (NaF-KF-UF4) Eutectics

Author

Maxime Fache, Laura Voigt, Jean-Yves Colle, John Hald, and Ondřej Beneš

Subjects
molten salt reactor, NaF-KF-UF4, fuel, thermophysical properties, density, vapor pressure, Technology, Electrical engineering. Electronics. Nuclear engineering, TK1-9971, Engineering (General). Civil engineering (General), TA1-2040, Microscopy, QH201-278.5, Descriptive and experimental mechanics, QC120-168.85
Abstract

General interest in the deployment of molten salt reactors (MSRs) is growing, while the available data on uranium-containing fuel salt candidates remains scarce. Thermophysical data are one of the key parameters for reactor design and understanding reactor operability. Hence, filling in the gap of the missing data is crucial to allow for the advancement of MSRs. This study provides novel data for two eutectic compositions within the NaF-KF-UF4 ternary system which serve as potential fuel candidates for MSRs. Experimental measurements include their melting point, density, fusion enthalpy, and vapor pressure. Additionally, their boiling point was extrapolated from the vapor pressure data, which were, at the same time, used to determine the enthalpy of vaporization. The obtained thermodynamic values were compared with available data from the literature but also with results from thermochemical equilibrium calculations using the JRCMSD database, finding a good correlation, which thus contributed to database validation. Preliminary thoughts on fluoride salt reactor operability based on the obtained results are discussed in this study.

Published
2024
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2. Metabolic Requirements for Spermatogonial Stem Cell Establishment and Maintenance In Vivo and In Vitro

Author

Nathalia de Lima E Martins Lara, Ina Dobrinski, Shiama Thiageswaran, and Anna Laura Voigt

Subjects
0301 basic medicine, Adult, Male, Cell Culture Techniques, Context (language use), Review, Biology, prepubertal development, Catalysis, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, Animals, Humans, Physical and Theoretical Chemistry, Spermatogenesis, spermatogonial culture, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Cells, Cultured, 030219 obstetrics & reproductive medicine, Adult Germline Stem Cells, Organic Chemistry, Translational medicine, Cell Differentiation, General Medicine, In vitro, Spermatogonia, Computer Science Applications, Cell biology, spermatogonial maturation, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), lcsh:QD1-999, Stem cell, metabolism, Germ cell, Adult stem cell
Abstract

The spermatogonial stem cell (SSC) is a unique adult stem cell that requires tight physiological regulation during development and adulthood. As the foundation of spermatogenesis, SSCs are a potential tool for the treatment of infertility. Understanding the factors that are necessary for lifelong maintenance of a SSC pool in vivo is essential for successful in vitro expansion and safe downstream clinical usage. This review focused on the current knowledge of prepubertal testicular development and germ cell metabolism in different species, and implications for translational medicine. The significance of metabolism for cell biology, stem cell integrity, and fate decisions is discussed in general and in the context of SSC in vivo maintenance, differentiation, and in vitro expansion.

Published
2021

3. Loss of Ubiquitin Carboxy-Terminal Hydrolase L1 Impairs Long-Term Differentiation Competence and Metabolic Regulation in Murine Spermatogonial Stem Cells

Author

Ina Dobrinski, Lin A. Tang, Lin Su, Rkia Dardari, Timothy E. Shutt, Iman Al Khatib, Wisoo Shin, Krysta M. Coyle, Taylor Goldsmith, Claudia Klein, Anna Laura Voigt, Jeff Biernaskie, and Whitney Alpaugh

Subjects
Male, endocrine system, QH301-705.5, Oxidative phosphorylation, Article, Transcriptome, Mice, 03 medical and health sciences, mitochondrial capacity, 0302 clinical medicine, Ubiquitin, UCH-L1, Animals, Biology (General), Progenitor cell, Spermatogenesis, Cells, Cultured, 030304 developmental biology, Mice, Knockout, 0303 health sciences, biology, Sequence Analysis, RNA, urogenital system, Stem Cells, murine spermatogenesis, Gene Expression Regulation, Developmental, Cell Differentiation, General Medicine, Ubiquitin carboxy-terminal hydrolase L1, Spermatogonia, Mitochondria, Cell biology, Mice, Inbred C57BL, Transplantation, biology.protein, Female, metabolic regulation, Single-Cell Analysis, germ cell differentiation, Ubiquitin Thiolesterase, 030217 neurology & neurosurgery, Homeostasis
Abstract

Spermatogonia are stem and progenitor cells responsible for maintaining mammalian spermatogenesis. Preserving the balance between self-renewal of spermatogonial stem cells (SSCs) and differentiation is critical for spermatogenesis and fertility. Ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1) is highly expressed in spermatogonia of many species, however, its functional role has not been identified. Here, we aimed to understand the role of UCH-L1 in murine spermatogonia using a Uch-l1−/− mouse model. We confirmed that UCH-L1 is expressed in undifferentiated and early-differentiating spermatogonia in the post-natal mammalian testis. The Uch-l1−/− mice showed reduced testis weight and progressive degeneration of seminiferous tubules. Single-cell transcriptome analysis detected a dysregulated metabolic profile in spermatogonia of Uch-l1−/− compared to wild-type mice. Furthermore, cultured Uch-l1−/− SSCs had decreased capacity in regenerating full spermatogenesis after transplantation in vivo and accelerated oxidative phosphorylation (OXPHOS) during maintenance in vitro. Together, these results indicate that the absence of UCH-L1 impacts the maintenance of SSC homeostasis and metabolism and impacts the differentiation competence. Metabolic perturbations associated with loss of UCH-L1 appear to underlie a reduced capacity for supporting spermatogenesis and fertility with age. This work is one step further in understanding the complex regulatory circuits underlying SSC function.

Published
2021

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