Search

Estimating the location of unmanned aerial vehicles (UAVs) within a global coordinate system can be achieved by correlating known world points with their corresponding image projections captured by the vehicle’s camera. Reducing the number of required world points may lower the computational requirements needed for such estimation. This paper introduces a novel method for determining the absolute position of aerial vehicles using only two known coordinate points that reduce the calculation complexity and, therefore, the computation time. The essential parameters for this calculation include the camera’s focal length, detector dimensions, and the Euler angles for Pitch and Roll. The Yaw angle is not required, which is beneficial because Yaw is more susceptible to inaccuracies due to environmental factors. The vehicle’s position is determined through a sequence of straightforward rigid transformations, eliminating the need for additional points or iterative processes for verification. The proposed method was tested using a Digital Elevation Model (DEM) created via LiDAR and 11 aerial images captured by a UAV. The results were compared against Global Navigation Satellite Systems (GNSSs) data and other common image pose estimation methodologies. While the available data did not permit precise error quantification, the method demonstrated performance comparable to GNSS-based approaches.

Improving the availability of new sources of probiotics is essential to continue implementing alternative solutions to improve coral health; one such source is urban corals. However, little is known about urban coral’s health status and whether they can harbor bacteria exhibiting probiotic traits. Here, we explored the status of the urban coral Madracis auretenra and the presence of probiotic traits in its associated culturable bacteria. After assessing ecological attributes, we observed a similar abundance (cover %) when comparing patches of M. auretenra occurring in both an urban site and a marine protected area. The urban patch also exhibited a high abundance of vibrios in coral tissues and signs of ecosystem deterioration. However, this patch showed a “good” health index condition; so, we hypothesized the presence of beneficial bacteria. We isolated 132 bacterial strains from this healthy urban M. auretenra. These bacteria were affiliated with 11 genera, including Vibrio, Shewanella, Bacillus, Exiguobacterium, Priestia, and Niallia, among others. Screenings revealed the predominant presence of potential probiotic traits such as catalase, antiQS, and the production of siderophores activities among the bacterial isolates. We proposed a list of 24 bacterial isolates as probiotic precandidates that jointly exhibited three or more of these traits, among which the Vibrio and Bacilli strains stand out. We provide insights into the health status of this urban coral and its potential as a source of bacteria exhibiting potential probiotic traits.

Information about the current biomass state of crops is important to evaluate whether the growth conditions are adequate in terms of water and nutrient supply to determine if there is need to react to diseases and to predict the expected yield. Passive optical Unmanned Aerial Vehicle (UAV)-based sensors such as RGB or multispectral cameras are able to sense the canopy surface and record, e.g., chlorophyll-related plant characteristics, which are often indirectly correlated to aboveground biomass. However, direct measurements of the plant structure can be provided by LiDAR systems. In this study, different LiDAR-based parameters are evaluated according to their relationship to aboveground fresh and dry biomass (AGB) for a winter spelt experimental field in Dahmsdorf, Brandenburg, Germany. The parameters crop height, gap fraction, and LiDAR intensity are analyzed according to their individual correlation with AGB, and also a multiparameter analysis using the Ordinary Least Squares Regression (OLS) is performed. Results indicate high absolute correlations of AGB with gap fraction and crop height (−0.82 and 0.77 for wet and −0.70 and 0.66 for dry AGB, respectively), whereas intensity needs further calibration or processing before it can be adequately used to estimate AGB (−0.27 and 0.22 for wet and dry AGB, respectively). An important outcome of this study is that the combined utilization of all LiDAR parameters via an OLS analysis results in less accurate AGB estimation than with gap fraction or crop height alone. Moreover, future AGB states in June and July were able to be estimated from May LiDAR parameters with high accuracy, indicating stable spatial patterns in crop characteristics over time.

Monitoring of canopy density with related metrics such as leaf area index (LAI) makes a significant contribution to understanding and predicting processes in the soil–plant–atmosphere system and to indicating crop health and potential yield for farm management. Remote sensing methods using optical sensors that rely on spectral reflectance to calculate LAI have become more mainstream due to easy entry and availability. Methods with vegetation indices (VI) based on multispectral reflectance data essentially measure the green area index (GAI) or response to chlorophyll content of the canopy surface and not the entire aboveground biomass that may be present from non-green elements that are key to fully assessing the carbon budget. Methods with light detection and ranging (LiDAR) have started to emerge using gap fraction (GF) to estimate the plant area index (PAI) based on canopy density. These LiDAR methods have the main advantage of being sensitive to both green and non-green plant elements. They have primarily been applied to forest cover with manned airborne LiDAR systems (ALS) and have yet to be used extensively with crops such as winter wheat using LiDAR on unmanned aircraft systems (UAS). This study contributes to a better understanding of the potential of LiDAR as a tool to estimate canopy structure in precision farming. The LiDAR method proved to have a high to moderate correlation in spatial variation to the multispectral method. The LiDAR-derived PAI values closely resemble the SunScan Ceptometer GAI ground measurements taken early in the growing season before major stages of senescence. Later in the growing season, when the canopy density was at its highest, a possible overestimation may have occurred. This was most likely due to the chosen flight parameters not providing the best depictions of canopy density with consideration of the LiDAR’s perspective, as the ground-based destructive measurements provided lower values of PAI. Additionally, a distinction between total LiDAR-derived PAI, multispectral-derived GAI, and brown area index (BAI) is made to show how the active and passive optical sensor methods used in this study can complement each other throughout the growing season.

Monitoring the structure of forest stands is of high importance for forest managers to help them in maintaining ecosystem services. For that purpose, Unmanned Aerial Vehicles (UAVs) open new prospects, especially in combination with Light Detection and Ranging (LiDAR) technology. Indeed, the shorter distance from the Earth’s surface significantly increases the point density beneath the canopy, thus offering new possibilities for the extraction of the underlying semantics. For example, tree stems can now be captured with sufficient detail, which is a gateway to accurately locating trees and directly retrieving metrics—e.g., the Diameter at Breast Height (DBH). Current practices usually require numerous site-specific parameters, which may preclude their use when applied beyond their initial application context. To overcome this shortcoming, the machine learning Hierarchical Density-Based Spatial Clustering of Application of Noise (HDBSCAN) clustering algorithm was further improved and implemented to segment tree stems. Afterwards, Principal Component Analysis (PCA) was applied to extract tree stem orientation for subsequent DBH estimation. This workflow was then validated using LiDAR point clouds collected in a temperate deciduous closed-canopy forest stand during the leaf-on and leaf-off seasons, along with multiple scanning angle ranges. The results show that the proposed methodology can correctly detect up to 82% of tree stems (with a precision of 98%) during the leaf-off season and have a Maximum Scanning Angle Range (MSAR) of 75 degrees, without having to set up any site-specific parameters for the segmentation procedure. In the future, our method could then minimize the omission and commission errors when initially detecting trees, along with assisting further tree metrics retrieval. Finally, this research shows that, under the study conditions, the point density within an approximately 1.3-meter height above the ground remains low within closed-canopy forest stands even during the leaf-off season, thus restricting the accurate estimation of the DBH. As a result, autonomous UAVs that can both fly above and under the canopy provide a clear opportunity to achieve this purpose.